Identification of Phytochemical Compounds of Ethyl Acetate Extract of Bulung Anggur (Caulerpa Sp.) By Gc-Ms And Toxicity Test On Artemia Salina Leach Shrimp Larvae
Abstract
Sea grape (Caulerpa sp.) is one of the potential types of seaweed. Sea grape potential as a natural ingredient for making medicine. Balinese people use sea grape for consumption. The content of bioactive compounds in sea grape is suspected to be toxic at certain doses. The purpose of this study was to determine the compound content of ethyl acetate extract of sea grape (Caulerpa sp.) and the toxicity on Artemia salina Leach. The identification of the bioactive compounds was carried out by Gas Chromatography-Mass Spectrophotometry and the toxicity test of extracts was carried out using the Brine Shrimp Lethality Test (BSLT) method. The results showed that the ethyl acetate extract of sea grape (Caulerpa sp.) contained 27 chemical compounds, 7 of which had a quality value > 70%, namely Propanoic acid, ethyl ester; n-Propyl acetate; sec-Butyl acetate; Toluene; Acetic acid, butyl ester; Bicyclo [4.2.0] octa-1, 3, 5-triene; and Styrene. The results of the toxicity test of Artemia salina Leach are toxic which had an LC50 value of 44,070 ppm so that they can be used as a larvicide
Downloads
References
Firdouse, S., Begum, H. F., & Alam, P. (2019). GC–MS Analysis of Bioactive Compounds Present in Marketed Herbal Formulation Pykure Capsule. Journal of Drug Delivery and Therapeutics, 9(5-s), 73-104.
Hamidi, M. R., B. Jovanova, and T. K. Panovska. (2014). Toxicological Evaluation of The Plant Product Using Brine Shrimp (Artemia salina L.). Model, Journal of Pharmaceutical, 60 (1): 9-18.
Marraskuranto, E., Nursid, M., Utami, S., Setyaningsih, I., & Tarman, K. (2021). Kandungan Fitokimia, Potensi Antibakteri dan Antioksidan Hasil Ekstraksi Caulerpa racemosa dengan Pelarut Berbeda. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan, 16(1), 1-10.
Marsaoli, M. (2004). Kandungan Bahan Organik, N-Alkana, Aromatik dan Total Hidrokarbon dalam Sedimen di Perairan Raha Kabupaten Muna Sulawesi Tenggara. Makara Sains, 3, 116-122.
Norliana, N., & Rabiah, R. (2021). Prarancangan Pabrik Butyl Acetate Dengan Proses Esterifikasi Dari Acetic Acid Dan Butanol Dengan Katalis Sulfuric Acid Kapasitas 26.000 Ton/Tahun. Jurnal Tugas Akhir Teknik Kimia, 4(2), 98-102.
Nurhayati, A. P. D., N. Abdulgani, R. Febrianto. (2006). Uji Toksisitas Ekstrak Alvaresi terhadap Artemia salina Leach. Sebagai Studi Pendahuluan Potensi Anti Kanker. Skripsi. Program Studi Biologi FMIPA Institut Teknologi Se puluh November. Surabaya. 1-9.
PubChem. (2005). n-Propyl acetate. https://pubchem.ncbi.nlm.nih.gov/ compound/Propyl-acetate.
Suirta, I. W., Puspawati, N. M., & Gumiati, N. K. (2007). Isolasi dan identifikasi senyawa aktif larvasida dari biji mimba (Azadirachta indika A. Juss) terhadap larva nyamuk demam berdarah (Aedes aegypti). Jurnal kimia, 1(1), 47-54.
Suptijah, P. (2002). Rumput laut: Prospek dan tantangannya. Makalah Pengantar Falsafah Sains (PPS702). Program Pasca Sarjana IPB, Bogor.
Tapotubun, A. M. (2018). Komposisi kimia rumput laut (Caulerpa lentillifera) dari perairan Kei Maluku dengan metode pengeringan berbeda. Jurnal Pengolahan Hasil Perikanan Indonesia, 21(1), 13-23.
Vidya, P., & Chadha, A. (2010). Pseudomonas cepacia lipase catalyzed esterification and transesterification of 3-(furan-2-yl) propanoic acid/ethyl xester: A comparison in ionic liquids vs hexane. Journal of Molecular Catalysis B: Enzymatic, 65(1-4), 68-72.
Wang, Y., Dai, A., Huang, S., Kuo, S., Shu, M., Tapia, C. P. & Huang, C. M. (2014). Propionic acid and its esterified derivative suppress the growth of methicillin-resistant Staphylococcus aureus USA300. Beneficial microbes, 5(2), 161-168.
