POTENSI FILTRAT Trichoderma asperellum TKD DALAM MENGHAMBAT KONTAMINASI Aspergillus parasiticus PADA BIJI KACANG TANAH

Abstract

iThis researchhaimed to determineethe potency off Trichoderma asperellum TKD ffiltrate in inhibiting Aspergillus parasiticus contamination in peanut seeds. The treatment given to A. parasiticus was done in vitro and in vivo. In vitro there were three types methods, dual culture  and the inhibition test of the T.asperellum TKD ifiltrate using the diffusion well and the colony method with concentrations of 40% (v/v), 50%(v/v), 60%(v/v),70%(v/v) and 80%(v/v). While in vivo, it was done on peanut seeds added  T.asperellumiTKD filtrate with some concentrations similar to the treatment given in vitro. The parameters measured in vitro test were determined through PIRG (Percentage Inhibition of Radial Growth) and diameter inhibition zone, in vivo test parameter was the  A. parasiticus colony populations. The results showed T.asperellum TKD filtrate significantly (P?0,05) was able to inhibit A. parasiticus growth iin vitro and iin vivo. The percentageeof T.asperellum TKD bland power against A.parasiticus in vitro with the highest dual culture method was 60.65%. In the method of colony and diffusion well, the concentration of 80% was the highest concentration in inhibiting A. parasiticus, which were 83.00±1.60% and 15.09±0.12 mm. T.asperellum TKD filtrate with a concentration of 80% added to peanut seeds had the highest inhibition on the number of population of A. parasiticus colonies with a percentage decrease of 88.3%.