PEMILIHAN PRIMER RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) PADA PCR (POLYMERASE CHAIN REACTION) TANAMAN KAMBOJA (Plumeria sp.)

Vanesa Martida; Made Pharmawati

Vanesa Martida Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Kampus Bukit Jimbaran, Bali, Indonesia
Made Pharmawati Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Kampus Bukit Jimbaran, Bali, Indonesia

Abstrak

There are many variation of Plumeria sp. that grown in Bali. The genetic identity of Plumeria sp. need to be analysed
using molecular study for plant breeding purpose. DNA extraction and primer selection are basic steps for molecular study
especially in identification and analysis of genetic diversity. The aim of this research was to determine RAPD primers
suitable for molecular analysis of Plumeria sp. This research used CTAB method with modification for DNA extraction. The
samples were young leaves of Plumeria sp. dried using silica gel. The primers used were produced by University of British
Columbia and Operon Primer Technology. The results showed that DNA concentration of Plumeria sp from dried leaves was
between 33-267 ng/?l. Out of seven primers tested, three primers UBC-127, UBC-250, and OPH-06 produced clear and
scorable amplification products for further analyses.
Keywords: DNA, Plumeria sp., RAPD primer

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Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Kampus Bukit Jimbaran, Bali,
Indonesia