UTILIZATION OF Garcinia mangostana L. PEEL EXTRACT WHICH CONTAINS TANIN IN PROTECTING TOFU CAKE PROTEIN
Abstract
The research objective was to determine the amount of tannin in mangosteen peel extract and the best dose of mangosteen peel extract in protecting tofu cake protein. Mangosteen peel flour is extracted using water at a ratio of 1: 5, 1:15, 1: 30, 1:45. Then mixed with the tofu cake and dried. Furthermore, in vitro testing was carried out using a completely randomized design followed by Duncan’s test. The study used 5 treatments by adding tofu pulp as a con- trol with each of which was repeated 4 times. The results showed that the mangosteen peel extract at a ratio of 1:45 produced the highest total tannins, namely 26.55g, and the lowest concentration of N-NH3 (P <0.05), namely 4.01 mM. Besides that, it also produced the lowest digestibility of dry matter and organic matter digestibility (P <0.05), with an average of 61.46% and 32%. The conclusion was that extraction at a ratio of 1:45 mangosteen peel flour and water produced the highest amount of dissolved tannins and resulted in the lowest N-NH3 value which can be used as a protein protection agent (bypass protein).
Downloads
References
Badan Pusat Statistik (BPS). 2015. Produksi Kedelai Menurut Provinsi (ton), 1993-2015. BPS - Statistics Indonesia, Indonesia
Badan Pusat Statistik (BPS). 2019. Impor Kedelai menu- rut Negara Asal Utama, 2010-2019. BPS - Statistics Indonesia, Indonesia
Barry, T.N., T.R. Manley, and S. J. Duncan. 1986. The Role condensed tannins in the nutritive value of lo- tus pendunculatus for sheep. 4 Sies of carbohydrate and protein digestions as influenced by dietary reac- tive protein concentration. Br. J. Nutr. 55:123-137.
Burns, R.E. 1971. Method for estimation of tannin in grain sorghum. Agronomy Journal 63: 511–12.
Harborne, J.B. and C.F. Van Sumere. 1975. Annual Por- ceding of the Phytoarteremical. Society Number Academic Press, Inc. London.
Hernaman, I., R. Hidayat, dan Mansyur. 2005. Pengaruh penggunaan molases dalam pembuatan silase cam- puran ampas tahu dan pucuk tebu kering terhadap nilai ph dan komposisi zat-zat makanannya. Jurnal Ilmu Ternak, 5 (2) : 94 – 99
Hernaman, I., U.H. Tanuwiria, dan M. F. Wiyatna. 2005. Pengaruh Penggunaan Berbagai Tingkat Kulit Kopi dalam Ransum Penggemukan Sapi Potong terhadap Fermentabilitas Rumen dan Kecernaan In-Vitro. Bionatura 7: 46-50.
Hernaman, I., A. Budiman, S. Nurachman, dan K. Hida- jat. 2015. Kajian in vitro subtitusi konsentrat dengan penggunaan limbah perkebunan singkong yang disuplementasi kobalt (Co) dan seng (Zn) dalam ransum domba. Buletin Peternakan 39 (2): 71-77
Jaya, J.K., L. Ariyani dan Hadijah. 2018. Perencanaan produksi bersih industri pengolahan tahu di UD. Sumber Urip Pelaihari. Jurnal Agroindustri 8 (2): 105-112
McDougall, E.I. 1948. Studies on ruminant saliva.1. The composition and output of sheep’s. Biochem. J. 43: 99-109.
McSweeney, C.S., B. Palmer, R. Bunch and D.O. Krause. 1999. Isolation dan characterization of proteolytic ruminal bacteria from sheep dan goats fed the Tannins-Containing shrub legum Calliandra calot- hyrsus. Applied and Environmental Microbiology, Juli 1999. p 3075-3083.
Miranti, T.T. Nugroho, dan H.Y. Teruna. 2016. Penen- tuan kadar tanin dalam pelarut etanol 50% dari kulit buah manggis (Garcinia mangostana L.) dengan bantuan selulase Trichoderma asperellum LBKURCC1. Photon: Jurnal Sain Dan Kesehatan, 6 (02), 7-11.
Shurtleff, W. and A. Aoyagi. 1975. The Book of Tohu, Food for Mankind. Ten Speed Press. California, USA.
Sudekum, K.H., S. Wolffram, P. Ader, and J.C. Robert. 2004. Bioavailability of three ruminally protected methionine sources in cattle. Anim. Feed Sci. Tech. 113 : 17-25.
Sutardi, T. 1983. Pengelolaan Tata Laksana Makanan dan Kesehatan Sapi Perah. Ceramah Ilmiah. Fapet. IPB Bogor.
Steel, R.G.D., dan J.H. Torrie. 1995. Prinsip dan Prosedur Statistika. Edisi ke-4. Penerbit Gramedia Pustaka Utama, Jakarta. (Diterjemahkan oleh B. Sumantri).
Tilley, J.M. and R.A.Terry 1963. A Two-Stage Technique for the In Vitro Digestion of Forage Crops. J. Br. Grass. Soc., 18:105-111.
Zamsari, M., Sunarso, dan Sutrisno. 2012. Pemanfaatan tanin alami dalam memproteksi protein bungkil kelapa ditinjau dari fermentabilitas protein secara in vitro. Animal Agriculture Journal, 1 (1) : 405 – 416.
