Identification of Bacteria Caused Bacterial Wilt Disease in Potato Plants (Solanum tuberosum L.) in Taman Tanda Village, Tabanan, Bali

Abstrak

One of the main problems in potato cultivation in Taman Tanda Village is bacterial wilt disease caused by pathogenic bacteria. The cause of bacterial lay is bacteria from the Ralstoniaceae family. The purpose of this study was to identify the bacteria that cause bacterial wilt in potato plants in Taman Tanda Village, Tabanan, Bali. The method begins with the isolation of pathogens from diseased potato plants in the field using Ralstonia casein peptone glucose selective media. Colonies grown on selective media were then characterized morphologically and biochemically. The pathogenicity of the pathogen was tested by inoculation of the pathogen on 30-day-old potato plants in a greenhouse. Potato plants showing bacterial symptoms such as dry wilting on leaves and stems were confirmed as Ralstonia sp. The isolates were then extracted with 16S rRNA and amplified by the Polymerase Chain Reaction (PCR) method using U1 forward and reverse primers. 16S rRNA sequencing was carried out in one direction by sending DNA to PT. Genetics, Jakarta. The results of the sequencing were then analyzed by BLASTN by comparing the data in GenBank. BLASTN analysis showed the pathogen isolate as Ralstonia solanacearum strain K60-1 with 100% virtue. The dendogram of the pathogen isolates was made using the neighbor join tree method 1000 times bootstrap. Based on morphology, biochemistry, Koch's postulates, and molecular the cause of bacterial wilt in potato plants in Taman Tanda Village, Tabanan, Bali identified as Ralstonia solanacearum.