Antioxidant Test Leunca Plant Leaf Extract(Solanum nigrumL.)

Abstract

Leunca plant (Solanum nigrum L.) is one of the plants that is often used as food for the people of Indonesia. Leunca plant leaves are widely used for herbal medicines. This study aims to determine the phytochemical content and antioxidant activity of leunca leaf extract (Solanum nigrum L.). The study was conducted at the Analytical Laboratory of Udayana University, the Organic Laboratory of Chemistry Study Programs at Udayana University and the Laboratory of the Faculty of Agriculture of Udayana University. Phytochemical tests were carried out to determine the presence of alkaloid compounds, steroids / triterpenoids, flavonoids, phenols and tannins. The antioxidant activity of leunca leaf extract (Solanum nigrum Linn) was tested by DPPH method (1-1-diphenyl-2-picrilhidrazil), with 5 different concentrations and consisted of 4 replications. Leunca positive leaf extract contains alkaloids, steroids / triterpenoids, flavonoids, phenols and tannins. The results showed that dried leunca leaf extract and fresh leunca leaf extract had different antioxidant activity. Dry leunca leaf extract is classified as strong antioxidant with antioxidant activity value of 64.40%, IC₅₀ value of 95.12 ppm and fresh leunca leaf extract is classified as weak antioxidant with antioxidant activity value of 16.83%, IC₅₀ value of 243.66 ppm.

Keywords: Solanum nigrum L., herb, phytochemicals, antioxidant, DPPH