STUDI HISTOPATOLOGI INSANG IKAN NILA (Oreochromis niloticus, Linn.) DITINJAU DARI KADAR AMMONIA (NH3) DI DANAU BATUR, BALI

Abstrak

Lake Batur is the largest lake on the island of Bali which is located in the district of Kintamani, Bangli regency. Tilapia fish gills are used as indicators for an organ that is extremely sensitive to changes in water quality. The study aims to determine the condition of the aquatic environment and the picture of the gills histopathologyof Tilapia in terms of levels of Ammonia (NH₃). The research was conducted in January to March 2016 with the method used were direct observation and purposive sampling at five stations in Lake Batur. Histopathological analyses were performed in the laboratory of histopathology, Fish Quarantine and Quality Control class 1 Denpasar, Bali. Gill histopathology samples were analyzed through a process of isolation, fixation, dehydration and embedding in paraffin and staining with Haematoxylin-Eosin. Results of water quality showed the value of 8.71 to 9.08 pH, temperature values ??from 25.4 to 30.0°C, DO levels of 3.8 to 5.1 mg/L is still on the brink of class III quality standards for fishing activities, NH₃ levels from 0.027 to 0.105 mg/L which is above the threshold value of class III water quality standard for fishing activities refer to Regulation No. 81 Year 2001 on Water Quality Management and Water Pollution. The observation of histopathology showed changes in terms of the levels of NH₃. Changes that occur in the gills in the form of fusion, hyperplasia, apoptosis, telengiektasis and haemoragii.