INDUKSI PERTUMBUHAN TUNAS DARI EKSPLAN ANGGREK Dendrobium Heterocarpum Lindl. DENGAN PEMBERIAN HORMON ZEATIN DAN NAA

  • Ni Luh Putu Kayika Febryanti
  • Made Ria Defiani
  • Ida Ayu Astarini

Abstract

Dendrobium heterocarpum Lindl. is a native orchid in Indonesia. Its population continue to decline due to unsustainable exploitation. One solution to increase its population is via in vitro technique propagation. This research aimed to determine effect of hormone addition (Zeatin and NAA) to induce shoot multiplication of D. heterocarpum Lindl. grown in Murashige and Skoog (MS) basic medium. The research was conducted in Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Science, Udayana University from November 2015 until March 2016. The experiment employed Completely Randomized Design (CRD) with 20 combinations of treatments, with three replicates each treatment. The treatment werer addition of Zeatin and NAA, with five concentrations of Zeatin (0, 0.1, 0,5, sweet corn and hybrids corn as the natural Zeatin); four NAA concentrations (0, 0.05, 0.1 and 0.5). Variable recorded include such as the number of shoots, roots and explant’s vigor. The quantitative data which include number of leaves and shoots after 12 weeks, were analyzed using Analysis of Variance (ANOVA); and if significantly different, tested further using Duncan Multiple Range Test (DMRT) at 5% level. Z5N3 medium revealed significantly different on amount of leaves and shoot height and also the best medium on amount of roots. Best hormone combination that can induce multiplication of new shoots are medium Z4N3 and Z5N1.

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Author Biographies

Ni Luh Putu Kayika Febryanti

Program Studi Biologi, Fakultas MIPA, Universitas Udayana, Jln. Kampus Bukit Jimbaran, Bali, Indonesia

Made Ria Defiani

Program Studi Biologi, Fakultas MIPA, Universitas Udayana Jln. Kampus Bukit Jimbaran, Bali, Indonesia

Ida Ayu Astarini
Program Studi Biologi, Fakultas MIPA, Universitas Udayana Jln. Kampus Bukit Jimbaran, Bali, Indonesia
Published
2017-03-31
How to Cite
FEBRYANTI, Ni Luh Putu Kayika; DEFIANI, Made Ria; ASTARINI, Ida Ayu. INDUKSI PERTUMBUHAN TUNAS DARI EKSPLAN ANGGREK Dendrobium Heterocarpum Lindl. DENGAN PEMBERIAN HORMON ZEATIN DAN NAA. Metamorfosa: Journal of Biological Sciences, [S.l.], v. 4, n. 1, p. 41-47, mar. 2017. ISSN 2302-5697. Available at: <https://ojs.unud.ac.id/index.php/metamorfosa/article/view/29748>. Date accessed: 27 feb. 2021. doi: https://doi.org/10.24843/metamorfosa.2017.v04.i01.p07.
Section
Articles

Keywords

shoots multiplication, in vitro technique, D. heterocarpum Lindl,.plant hormone

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