Kualitas Tulang Tikus Betina Normal yang Diberi Ekstrak Sipatah-patah pada Masa Pertumbuhan

  • Sabri Mustafa
  • Nurhidayat -
  • Koeswinarning Sigit
  • Bambang Pontjo Priosoeryanto
  • Wasmen Manalu

Abstract

The experiment was designed to study the effects of sipatah-patah extract (ESP) on bone growth ingrowing female rats in order to prevent osteoporosis during postmenopausal period. Twenty growing femaleSprague Dawley rats in similar body weight were used with the average of age was 20 days old. Theexperimental rats were randomly divided into 5 (five) groups: control group (NOV-0, administered carboxylmethyl cellulose 1%) and groups administered ESP at the age of 30 days (NOV-1), 60 days (NOV-2), 90days (NOV-3), and 120 days (NOV-4) with dosage of 750 mg/kg body weight daily. Blood samples werecollected every 30 days to analyze serum calcium and phosphate concentrations. At the end of the treatment(180 day old), all of the animals were sacrificed to measure and analyze bone growth histologically. Ostibia-fibula slice was stained by using Hematoxylin Eosin (HE) method to observe osteoblast and osteoclastdensities, while Masson trichrome was used to observe trabeculae structure. The results showedadministration of ESP in rat during growing period improved bone growth. NOV-1 with longer duration(150 days) showed better growth rate with longer femur, optimum serum calcium, and phosphateconcentrations, and higher number of osteoblast with lower osteoclast densities compared to control rats(NOV-0). It was concluded that giving ESP at earlier age with longer duration during growth period couldimprove bone growth. This condition was expected could also improve the bone conditions during growingperiod.

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Author Biographies

Sabri Mustafa
Laboratorium Anatomi, Fakultas Kedokteran Hewan, Universitas Syiah Kuala,Jln. T. Hasan Krueng Kalee No. 4, Telepon 0651 51977, Darussalam Banda Aceh
Nurhidayat -
Departemen Anatomi Fisiologi dan Farmakologi FKH IPB Bogor
Koeswinarning Sigit
Departemen Anatomi Fisiologi dan Farmakologi FKH IPB Bogor
Bambang Pontjo Priosoeryanto
Departemen Klinik Reproduksi dan Patologi FKH IPB Bogor
Wasmen Manalu
Departemen Anatomi Fisiologi dan Farmakologi FKH IPB Bogor
How to Cite
MUSTAFA, Sabri et al. Kualitas Tulang Tikus Betina Normal yang Diberi Ekstrak Sipatah-patah pada Masa Pertumbuhan. Jurnal Veteriner, [S.l.], p. 113-119, nov. 2012. ISSN 2477-5665. Available at: <https://ojs.unud.ac.id/index.php/jvet/article/view/3486>. Date accessed: 30 aug. 2026.
Citation Formats
Issue
Vol 12, No 2 (2011)
Section
Articles

Keywords

sipatah-patah extract, osteoblast, osteoclast, trabeculae, serum calcium and phosphate.
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This work is licensed under a Creative Commons Attribution 4.0 International License