Aktivitas Antibakteri Krim Ekstrak Terpurifikasi Daun Beluntas (Plucea indica L.) terhadap Staphylococcus epidermidis
Abstract
Staphylococcus epidermidis merupakan bakteri flora normal kulit. Ketidaknormalan flora normal akan menyebabkan gangguan kulit diantaranya jerawat. Pengembangan kosmesetika sebagai antibakteri perlu terus dilakukan salah satunya menggunakan bahan alam daun beluntas. Beluntas merupakan tanaman yang memiliki aktivitas sebagai antibakteri dan anti inflamasi karena mengandung metabolit sekunder seperti tannin, polifenol, flavonoid dan minyak atsiri. Ekstrak dilakukan purifikasi untuk memperkecil massa ekstrak. Tujuan penelitian dilakukan untuk mengevaluasi ekstrak terpurifikasi daun beluntas dalam formulasi krim sebagai kandidat kosmesetika yang dapat menghambat pertumbahan bakteri S. epidermidis. Sediaan krim dilakukan pengamatan karakteristik fisik untuk melihat stabilitas krim dalam penyimpanan. Sediaan krim dilakukan uji antibakteri terhadap S. epidermidis menggunakan metode difusi. Krim yang mengandung ekstrak daun beluntas 5% b/v, 10% b/v dan 15% b/v memiliki aktivitas antibakteri terhadap S. epidermidis dengan diameter zona hambat berturut – turut sebesar 9,9±0,022 mm (sedang), 14,9±0,019 mm (kuat), dan 20,9±0,049 mm (sangat kuat). Krim daun beluntas tidak mengalami perubahan pada pengujian organoleptis. Pengujian nilai pH dan viskositas mengalami peningkatan tetapi masih memenuhi kriteria sediaan krim.
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References
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Brooks, G., Carroll, K., Butel, J., Morse, S., & Mietzner, T. (2012). Jawetz, Melnick & Adelberg’s Mikrobiologi Kedokteran Medical Microbiology (25 th edit). Jakarta: Penerbit Buku Kedokteran EGC.
Buranasukhon, W., Athikomkulchai, S., Tadtong, S., & Chittasupho, C. (2017). Wound healing activity of Pluchea indica leaf extract in oral mucosal cell line and oral spray formulation containing nanoparticles of the extract. Pharmaceutical Biology, 55(1), 1767–1774. https://doi.org/10.1080/13880209.2017.1326511
Chen, M. X., Alexander, K. S., & Baki, G. (2016). Formulation and Evaluation of Antibacterial Creams and Gels Containing Metal Ions for Topical Application. Journal of Pharmaceutics, 2016, 1–10. https://doi.org/10.1155/2016/5754349
Darojah, P., Santoso, O., & Ciptaningtyas, V. R. (2019). Pengaruh Asap Cair Berbagai Konsentrasi Terhadap Viabilitas Staphyloococcus Epidermidis. Diponegoro Medical Journal (Jurnal Kedokteran Diponegoro), 8(1), 390–400.
Donowarti, I., & Dayang Diah, F. (2020). Pengamatan hasil olahan daun beluntas (Pluchea indica L.) terhadap sifat fisika dan kimianya. Teknologi Pangan : Media Informasi Dan Komunikasi Ilmiah Teknologi Pertanian, 11(2), 118–134. https://doi.org/10.35891/tp.v11i2.2166
Ermawati, D. E., Yugatama, A., & Wulandari, W. (2020). Uji Sifat Fisik, Sun Protecting Factor, dan In Vivo ZnO Terdispersi dalam Sediaan Nanoemulgel. JPSCR: Journal of Pharmaceutical Science and Clinical Research, 5(1), 49. https://doi.org/10.20961/jpscr.v5i1.31660
Hidayat, R., & Alhadi, F. (2012). Identifikasi Streptococcus Equi dari Kuda yang Diduga Menderita Strangles Identification Streptococcus Equi from Horses Suspected Strangles. Jurnal Ilmu Pertanian Indonesia (JIPI), 17(3), 199–203.
Ikalinus, R., Widyastuti, S., & Eka Setiasih, N. (2015). Skrining Fitokimia Ekstrak Etanol Kulit Batang Kelor (Moringa Oleifera). Indonesia Medicus Veterinus, 4(1), 71–79.
Kalishwaralal, K., BarathManiKanth, S., Pandian, S. R. K., Deepak, V., & Gurunathan, S. (2010). Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis. Colloids and Surfaces B: Biointerfaces, 79(2), 340–344. https://doi.org/10.1016/j.colsurfb.2010.04.014
Lai, Y., Cogen, A. L., Radek, K. A., Park, H. J., MacLeod, D. T., Leichtle, A., … Gallo, R. L. (2010). Activation of TLR2 by a small molecule produced by staphylococcus epidermidis increases antimicrobial defense against bacterial skin infections. Journal of Investigative Dermatology, 130(9), 2211–2221. https://doi.org/10.1038/jid.2010.123
Lailiyah, M., Saputra, S. A., & Sari, F. (2020). Antioxidant activity and sun protection factor evaluation for cream formulation of purified roasted corn silk extracts (Zea Mays L. Saccharata). Pharmaciana, 10(3), 371. https://doi.org/10.12928/pharmaciana.v10i3.17780
Lestari, I., Lestari, U., & Gusti, D. R. (2018). Antioxidant activity and irritation test of peel off gel mask of ethanol extract of pedada fruit ( Sonneratia caseolaris ). International Conference on Pharmaceutical Research and Practice, 978–979.
Mahendran, S., & Abdul Rashid, N. (2016). Formulation, evaluation and antibacterial properties of herbal ointment containing methanolic extract of Clinacanthus nutans leaves. International Journal of Pharmaceutical and Clinical Research, 8(8), 1170–1174.
Malik, A., Ahmad, A. R., & Najib, A. (2013). Daun Teh Hijau Dan Jati Belanda. Jurnal Fitofarmaka Indonesia, 4(2), 238–240.
Malik, A., Edward, F., & Waris, R. (2016). Skrining Fitokimia dan Penetapan Kandungan Flavonoid Total Ekstrak Metanolik Herba Boroco (Celosia argentea L.). Jurnal Fitofarmaka Indonesia, 1(1), 1–5. https://doi.org/10.33096/jffi.v1i1.193
Meila, O., Pontoan, J., Uun, H. W., & Pratiwi, A. (2017). Formulasi Krim Ekstrak Etanol Daun Beluntas (PLuchea indica L.,) dan Uji Kestabilan Fisiknya. Indonesian Natural Research Pharmaceutical Journal, 1(2), 95–106.
Mohamed, S., Elmohamady, M. N., Abdelrahman, S., Amer, M. M., & Abdelhamid, A. G. (2020). Antibacterial effects of antibiotics and cell-free preparations of probiotics against Staphylococcus aureus and Staphylococcus epidermidis associated with conjunctivitis. Saudi Pharmaceutical Journal, 28(12), 1558–1565. https://doi.org/10.1016/j.jsps.2020.10.002
Nakatsuji, T., Chen, T. H., Butcher, A. M., Trzoss, L. L., Nam, S. J., Shirakawa, K. T., … Gallo, R. L. (2018). A commensal strain of Staphylococcus epidermidis protects against skin neoplasia. Science Advances, 4(2). https://doi.org/10.1126/sciadv.aao4502
Nurhidayati, S., Faturrahman, F., & Ghazali, M. (2015). Deteksi Bakteri Patogen yang Berasosiasi dengan Kappaphycus alvarezii (Doty) Bergejala Penyakit Ice - Ice. Jurnal Sains Teknologi & Lingkungan, 1(2), 24–30. https://doi.org/10.29303/jstl.v1i2.53
Pargaputri, A. F., Munadziroh, E., & Indrawati, R. (2017). Antibacterial effects of Pluchea indica Less leaf extract on E. faecalis and Fusobacterium nucleatum (in vitro). Dental Journal (Majalah Kedokteran Gigi), 49(2), 93. https://doi.org/10.20473/j.djmkg.v49.i2.p93-98
Pratasik, M. C. M., Yamlean, P. V. Y., & Wiyono, W. I. (2019). Formulasi Dan Uji Stabilitas Fisik Sediaan Krim Ekstrak Etanol Daun Sesewanua (Clerodendron Squamatum Vahl.). Pharmacon, 8(2), 261–267. https://doi.org/10.35799/pha.8.2019.23488
Rasyid, A. U. M., & Amody, Z. (2020). Pengujian Efektivitas Formula Gel Ekstrak Daun Beluntas (Pluchea indica (L.) Less) Dengan Variasi Konsentrasi Gelling Agent Sebagai Kandidat Sediaan Anti Jerawat. Jurnal Ilmiah Manuntung, 6(2), 312. https://doi.org/10.51352/jim.v6i2.393
Tang, X., Shen, Y., Hu, R., Yang, T., Benghezal, M., Li, H., & Tang, H. (2020). Re-assessment of the disk diffusion technique for routine antimicrobial susceptibility testing for Helicobacter pylori. Helicobacter, 25(4), 1–7. https://doi.org/10.1111/hel.12703
Wiendarlina, I. Y., Indriati, D., & Rosa, M. (2019). Aktivitas Antibakteri Losion Anti Jerawat yang Mengandung Ekstrak Daun Beluntas (Pluchea indica (L) Less.). FITOFARMAKA: Jurnal Ilmiah Farmasi, 9(1), 16–25. https://doi.org/10.33751/jf.v9i1.1256
Yuliani, H., & Rasyid, M. I. (2019). Efek Perbedaan Pelarut terhadap Uji Toksisitas Ekstrak Pineung Nyen Teusalee. Jurnal Fitofarmaka Indonesia, 6(2), 347–352. https://doi.org/10.33096/jffi.v6i2.453
Zou, Y., Lu, Y., & Wei, D. (2004). Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. in vitro. Journal of Agricultural and Food Chemistry, 52(16), 5032–5039. https://doi.org/10.1021/jf049571r
Zulkarya, L. G., & Hastuti, E. D. (2018). Formulasi Sediaan Krim Ekstrak Etanol Rumput Laut Coklat (Padina australis) dan Uji Aktivitas Antioksidan Menggunakan DPPH. Cendekia Journal of Pharmacy, 2(1), 81–87. https://doi.org/10.31596/cjp.v2i1.20
Published
2022-07-30
How to Cite
ERWIYANI, Agitya Resti et al.
Aktivitas Antibakteri Krim Ekstrak Terpurifikasi Daun Beluntas (Plucea indica L.) terhadap Staphylococcus epidermidis.
Jurnal Farmasi Udayana, [S.l.], p. 8-14, july 2022.
ISSN 2622-4607.
Available at: <https://ojs.unud.ac.id/index.php/jfu/article/view/74398>. Date accessed: 18 apr. 2024.
doi: https://doi.org/10.24843/JFU.2022.v11.i01.p02.
Section
Articles
