Aktivitas Antimikroba Dan Antioksidan Ekstrak Dan Fraksi Daun Tembelekan (Lantana camara L.)
Abstract
Lantana camara is a medicinal plant belongs to family Verbenaceae. It is widely used as traditional medicine for treating various health problems. But there has been no report about potential activity of L. camara that grow in Indonesia and its fractions. The aim of this research were to investigate the antimicrobial activity of extract and its fractions of L. camara leaves in against . Extract was prepared by maceration in ethanol (96%) then fractionated using n-hexane and ethyl acetate. Antimicrobial activity was carried out using agar difussion method and antioxidant activity was carried out using DPPH radical scavenging activity assay. Concentration of extract and fraction tested were 12,5 ?g/mL; 25 ?g/mL; 50 ?g/mL; and 100 ?g/mL. The result showed that extract and fraction of L. camara leaves exhibit strong antibacterial activity against Gram positive bacteria (Bacillus subtilis, Staphylococcus aureus), weak inhibition against Gram negative bacteria (Escherichia coli), and and were not able to inhibit the growth of Candida albicans. The ethanolic extract of L. camara leaves and its fractions had antioxidant activity. DPPH radical scavenging assay showed the IC50 value of 8.95 ?g / mL for ethanolic extract.
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References
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Zhang, Y.-J., Gan, R.-Y., Li, S., Zhou, Y., Li, A.-N., Xu, D.-P., & Li, H.-B. (2015). Antioxidant Phytochemicals for the Prevention and Treatment of Chronic Diseases. Molecules, 20(12), 21138–21156. https://doi.org/10.3390/molecules201219753
Zhu, J., Yi, X., Zhang, J., Chen, S., & Wu, Y. (2017). Chemical profiling and antioxidant evaluation of Yangxinshi Tablet by HPLC–ESI-Q-TOF-MS/MS combined with DPPH assay. Journal of Chromatography B, 1060, 262–271. https://doi.org/10.1016/j.jchromb.2017.06.022
Zulqarnain, Rahim A, Ahmad K, Ullah F, Ullah H, & Nishan U. (2015). In vitro antibacterial activity of selected medicinal plants from lower Himalayas. 28(2), 581‐587
Dawidowicz, A. L., Wianowska, D., & Olszowy, M. (2012). On practical problems in estimation of antioxidant activity of compounds by DPPH method (Problems in estimation of antioxidant activity). Food Chemistry, 131(3), 1037–1043. https://doi.org/10.1016/j.foodchem.2011.09.067
Foti, M. C. (2015). Use and Abuse of the DPPH • Radical. Journal of Agricultural and Food Chemistry, 63(40), 8765–8776. https://doi.org/10.1021/acs.jafc.5b03839
Gomes de Melo, J., De Sousa Araújo, T. A., Thijan Nobre de Almeida e Castro, V., Lyra de Vasconcelos Cabral, D., Do Desterro Rodrigues, M., Carneiro do Nascimento, S., Cavalcanti de Amorim, E. L., & De Albuquerque, U. P. (2010). Antiproliferative Activity, Antioxidant Capacity and Tannin Content in Plants of Semi-Arid Northeastern Brazil. Molecules, 15(12), 8534–8542. https://doi.org/10.3390/molecules15128534
Grace-Lynn, C., Darah, I., Chen, Y., Latha, L. Y., Jothy, S. L., & Sasidharan, S. (2012). In Vitro Antioxidant Activity Potential of Lantadene A, a Pentacyclic Triterpenoid of Lantana Plants. Molecules, 17(9), 11185–11198. https://doi.org/10.3390/molecules170911185
Irianti, T., Puspitasari, A., Machwiyyah, L., & Rabbani, H. R. (2016). THE ACTIVITY OF RADICAL SCAVENGING OF 2, 2-DIPHENYL-1-PYCRILHYDRAZIL (DPPH) BY ETHANOLIC EXTRACTS OF MENGKUDU LEAVES (Morinda citrifolia L.), BROTOWALI STEM (Tinospora crispa L.), ITS WATER FRACTION AND ITS HYDROLIZED FRACTION. Traditional Medicine Journal, 20(3), 140–148.
Jamal, M., Amir, M., Ali, Z., & Mujeeb, M. (2018). A comparative study for the extraction methods and solvent selection for isolation, quantitative estimation and validation of ursolic acid in the leaves of Lantana camara by HPTLC method. Future Journal of Pharmaceutical Sciences. https://doi.org/10.1016/j.fjps.2018.07.002
Kirimuhuzya, C., Waako, P., Joloba, M., & Odyek, O. (2009). The anti-mycobacterial activity of Lantana camara a plant traditionally used to treat symptoms of tuberculosis in South-western Uganda. 9(1), 6.
Kumar, S., Sandhir, R., & Ojha, S. (2014). Evaluation of antioxidant activity and total phenol in different varieties of Lantana camara leaves. BMC Research Notes, 7(1), 560. https://doi.org/10.1186/1756-0500-7-560
Kumar, V. P., Chauhan, N. S., Padh, H., & Rajani, M. (2006). Search for antibacterial and antifungal agents from selected Indian medicinal plants. Journal of Ethnopharmacology, 107(2), 182–188. https://doi.org/10.1016/j.jep.2006.03.013
Mahdi-Pour, B., Jothy, S. L., Latha, L. Y., Chen, Y., & Sasidharan, S. (2012). Antioxidant activity of methanol extracts of different parts of Lantana camara. Asian Pacific Journal of Tropical Biomedicine, 2(12), 960–965. https://doi.org/10.1016/S2221-1691(13)60007-6
Pradeep BV, Tejaswini M, Nishal P, Pardhu G, Shylaja S, & Kumar KCh. (2013). Phytochemical screening and antimicrobial activities of plant extract of Lantana camara. 34(3), 645‐649.
Roumeliotis, S., Roumeliotis, A., Dounousi, E., Eleftheriadis, T., & Liakopoulos, V. (2019). Dietary Antioxidant Supplements and Uric Acid in Chronic Kidney Disease: A Review. Nutrients, 11(8), 1911. https://doi.org/10.3390/nu11081911
Sanhueza, L., Melo, R., Montero, R., Maisey, K., Mendoza, L., & Wilkens, M. (2017). Synergistic interactions between phenolic compounds identified in grape pomace extract with antibiotics of different classes against Staphylococcus aureus and Escherichia coli. PLoS ONE, 12(2), e0172273. PMC. https://doi.org/10.1371/journal.pone.0172273
Satyal P, Crouch RA, & Monzote L. (2016). The Chemical Diversity of Lantana camara: Analyses of Essential Oil Samples from Cuba, Nepal, and Yemen. 13(3), 336‐342.
Sharma, A., Flores-Vallejo, R. del C., Cardoso-Taketa, A., & Villarreal, M. L. (2017). Antibacterial activities of medicinal plants used in Mexican traditional medicine. Journal of Ethnopharmacology, 208, 264–329. https://doi.org/10.1016/j.jep.2016.04.045
Sousa, E. O., Miranda, C. M. B. A., Nobre, C. B., Boligon, A. A., Athayde, M. L., & Costa, J. G. M. (2015). Phytochemical analysis and antioxidant activities of Lantana camara and Lantana montevidensis extracts. Industrial Crops and Products, 70, 7–15. https://doi.org/10.1016/j.indcrop.2015.03.010
Swamy, M. K., Sinniah, U. R., & Akhtar, Mohd. S. (2015). In Vitro Pharmacological Activities and GC-MS Analysis of Different Solvent Extracts of Lantana camara Leaves Collected from Tropical Region of Malaysia. Evidence-Based Complementary and Alternative Medicine, 2015, 1–9. https://doi.org/10.1155/2015/506413
Thiericke, R. (2000). Drug discovery from Nature: Automated high-quality sample preparation. Journal of Analytical Methods in Chemistry, 22(5), 149–157.
Tsai, C.-E., & Lin, L.-H. (2019). DPPH scavenging capacity of extracts from Camellia seed dregs using polyol compounds as solvents. Heliyon, 5(8), e02315. https://doi.org/10.1016/j.heliyon.2019.e02315
Wahyuningrum, R., Utami, P. I., Dhiani, B. A., Kumalasari, M., & Kusumawardani, R. S. (2016). Screening of Potential Free Radicals Scavenger and Antibacterial Activities of Purwoceng (Pimpinella alpina Molk). Tropical Life Sciences Research, 27(3), 161–166. https://doi.org/10.21315/tlsr2016.27.3.22
Wink, M. (2015). Modes of Action of Herbal Medicines and Plant Secondary Metabolites. Medicines, 2(3), 251–286. https://doi.org/10.3390/medicines2030251
Wulandari, L., Nugraha, A. S., & Azhari, N. P. (2020). Penentuan Aktivitas Antioksidan dan Antidiabetes Ekstrak Daun Kepundung (Baccaurea racemosa Muell.Arg.) secara In Vitro. 07(01), 7.
Yeo, J., & Shahidi, F. (2019). Critical Re-Evaluation of DPPH assay: Presence of Pigments Affects the Results. Journal of Agricultural and Food Chemistry, 67(26), 7526–7529. https://doi.org/10.1021/acs.jafc.9b02462
Zhang, Y.-J., Gan, R.-Y., Li, S., Zhou, Y., Li, A.-N., Xu, D.-P., & Li, H.-B. (2015). Antioxidant Phytochemicals for the Prevention and Treatment of Chronic Diseases. Molecules, 20(12), 21138–21156. https://doi.org/10.3390/molecules201219753
Zhu, J., Yi, X., Zhang, J., Chen, S., & Wu, Y. (2017). Chemical profiling and antioxidant evaluation of Yangxinshi Tablet by HPLC–ESI-Q-TOF-MS/MS combined with DPPH assay. Journal of Chromatography B, 1060, 262–271. https://doi.org/10.1016/j.jchromb.2017.06.022
Zulqarnain, Rahim A, Ahmad K, Ullah F, Ullah H, & Nishan U. (2015). In vitro antibacterial activity of selected medicinal plants from lower Himalayas. 28(2), 581‐587
Published
2021-07-02
How to Cite
WAHYUNINGRUM, Retno; E, Genatrika; IN, Pahalawati.
Aktivitas Antimikroba Dan Antioksidan Ekstrak Dan Fraksi Daun Tembelekan (Lantana camara L.).
Jurnal Farmasi Udayana, [S.l.], p. 107-115, july 2021.
ISSN 2622-4607.
Available at: <https://ojs.unud.ac.id/index.php/jfu/article/view/69231>. Date accessed: 05 nov. 2024.
doi: https://doi.org/10.24843/JFU.2021.v10.i01.p13.
Section
Articles
