Profiling Senyawa Metabolit Sekunder Fraksi Aktif Antihiperkolesterolemia Dari Herba Ketumpang Air (Peperomia pellucida L)
Abstract
Herba ketumpang air (Peperomia pellucida L) contains bioactive compounds such as alkaloids, saponins, flavonoids, and steroids which are thought to be efficacious as antihypercholesterolemia. The purpose of this study was to determine the effectiveness of herba P. pellucida extracts as antihypercholesterolemia in vitro and characterize the active fraction. Extraction using ultrasonication methods with stratified organic solvents, such us n-hexane, ethyl acetate, and methanol. The fraction of n-hexane, ethyl acetate, and methanol were tested for cholesterol reduction activity in vitro by photometry method, cholesterol was reacted with Lieberman-Burchard reagents then analyzed using a UV-Vis spectrophotometer. The fraction that has the best activity is characterized by using a Liquid Chromatographic Mass Spectrometer (LCMS). Ethyl acetate fraction had the best activity to reduce cholesterol with a value EC50 of 196.43 ppm. Based on phytochemical tests the active fraction of ethyl acetate contains alkaloids, flavonoids, saponins, and steroids. Results of LCMS analysis of ethyl acetate fraction at a retention time of 4.97; 7.82; and 10.80 are thought to contain active compounds of the flavonoid group namely velutin, casticin, and dihydrochalcone.
Downloads
Download data is not yet available.
References
Amin MS. 2015. Studi In Vitro: Efek antikolesterol dari ekstrak metanol buah parijoto (Medinilla speciosa Blume) terhadap kolesterol total [skripsi]. Jakarta: Program studi Farmasi UIN Syarif Hidayatullah Jakarta.
Anggraini DI, dan Nabillah LF. 2018. Activity Test of Suji Leaf Extract (Dracaena angustifolia Roxb.) on In Vitro Cholesterol Lowering. Jurnal Kimia Sains dan Aplikasi. 21(2): 54-58
Asker E., Akin S., dan Hokelek T. 2006. 5,3’-Dihydroxy-3,6,7,4’ tetramethoxyflavone. Acta Crystallographica section E structure Reports Online. 62(9): o4519-o4161. https://doi.org/10.1515/ncrs-2017-0141
Attarde D., Pawar J., Chaudhari B., dan Pal S. 2010. Estimation of sterols content in edible oil and ghee samples. Internationa Journal of Pharmaceutical and Sciences Review and Research. 5(1): 135-137.
Baum JA., Teng H., Erdman JW., Weigel RM., Klein BP., Persky VW., Freels S., Surya P., Bakhit RM., Ramos E., Shay NF., dan Potter SM. 1998. Long-term intake of soy protein improves blood lipid profiles and icrases mononuclear cell low-density-lipoprotein receptor messenger RNA in hypercholesterolemic, postmenopausal women. The American Jurnal of Clinical Nutrituin. 68(3): 541-551. DOI: 10.1093/ajcn/68.3.545
Dalimartha S. 2002. Resep tumbuhan obat untuk menurunkan kolesterol. Jakarta : Penebar swadaya
Dalimarta S. 2006. Atlas Tumbuhan Obat Indonesia Jilid 4. Jakarta: Puspa Swara
Das KC., Farmer WJ., dan Winstein B. 1970. Phytochemical studies IX A new flavone velutin. J. Org. Chem.: 2970 95(11): 3989-3890. DOI: 10.1021/jo00836a101
Dipiro JT. 2005. Pharmacotherapy: A patophysiologic approach sixth edition edition. New York: McGraw-Hill Medical Publishing Division.
Fathoni A, Rudiana T, dan Adawiyah. 2019. Characterization and antioxidant assay of yellow frangipani flower (Plumeria alba) Extract. Jurnal Pendidikan Kimia. 1-7. DOI: 10.24114/jpkim.vlli1.13034
Gini T., dan Jothi J. 2013. Preminary phytochemichal screening of whole plant extracts of Peperomia pellucida (Linn.) HBK. (Piperaceae) and Marsilea quadrifolia Linn. (Marsileaceae). International Journal of Pharmacognosy and Phytochemical Research. 5(3): 200-214
Hadiarti D. 2017. Uji aktivitas ekstrak buas-buas (Premna serratifolia Linn) sebagai antikolesterol secara in vitro. Jurnal Ilmiah Ar-Raz. 5(1): 22-29. DOI: 10.29406/arz.v5i1.644
Hanani E. 2015. Analisis fitokimia. Jakarta: EGC Penerbit buku kedokteran
Januarti IB, Santoso A., dan Razak AS. 2017. Ekstraksi senyawa flavonoid daun jati (Tectona grandis L.) dengan metode ultrasonik (kajian rasio bahan: pelarut dan lama ekstraksi). Media Farmasi Indonesia. 12(2): 1259-1266
Judas N., Kaitner B., dan Mestrovic E. 2006. 1,3-Diphenylpropan-1-one. Acta Crystal-lographica Section E Structure Reports Online. 51(12): 2656-2658
Kang J., Xie C., Li Z., Nagarajan S., Schauss AG., Wu T., dan Wu X. 2011. Flavonoids from acai (Euterpe oleracea Mart.) pulp and their antioxidant and antiinflamatory activities. Food Chem. 128: 152-157. DOI: 10.1016/j/foodchem.2011.03.011
Majumder P., Abraham P., dan Stya V. 2011. Ethno-medicinal, phytochemical and pharmacological review of an amazing medicinal herb Peperomia pellucida (L.) HBK. Reseacrh Journal of Pharmaceutical, Biological, and Chemical. 2(4): 358-364.
Mawati DI. 2017. Uji aktivitas antihiperurisemia ekstrak etil asetat tanaman suruhan (Peperomia pellucida L. Kunth) pada tikus putih jantan yang dienduksi kafein [skripsi]. Jakarta: Program Studi Farmasi UIN Syarif Hidayatullah.
Mayes PA., Murray RK., Granner DK., dan Rodwell VW. 2003. Biokimia Harper ed. 25. Jakarta: Buku Kedokteran.
Mazroatul C., Deni GD., Habibi NA., Saputri GF. 2016. Anti-hypercholesterolemia activity of ethano extract Peperomia pellucida. Alchemy Jurnal Penelitian Kimia. 12(1): 86-94. https://doi.org/ 10.20961/alchemy.12.1.948.88.94
Mursu J., Nurmi T., Tuomainen TP., Ruusunen A., Salonen JT., dan Voutilainen S. 2007. The intake of flavonoids and carotid antheroscierosis: the kuopio ischaemic heart disease risk factory. Br. J. Nutr. 98: 814-818. DOI: 10.1017/S0007114507744410
Nuraeni F. dan Bernadetha S. 2018. Aktivitas antioksidan serta identifikasi senyawa dari ekstrak jamu lingzhi (Ganoderma lucidum) dengan liquid chromatography-mass spectrometry (LC-MS). Seminar Nasional Edusainstek FMIPA UNIMUS. 1-10
Priatna M., Sartika AM., Ambaryani R. 2015. Uji banding aktivitas antikolesterol ekstrak etanol buah pepiro (Solanum muricatum Ait) dan buah strawberry (Fragaria X ananassa Duchesne) pada tikus putih jantan. Jurnal Kesehatan Bakti Tunas Husada. 13(1): 165-172
Reddy MVB, Hung HY, Kuo PC, Huang GJ, Chan YY, Huang SC, Chan YY, Huang SC, Wu SJ, Morris NS, Lee KH, dan Wu TS. 2017. Synthesis and biological evaluation of chalcone, dyhydrochalcone, and 1,3-diarypropane analogs as anti-inflamatory agents. Bioorganic and medicinal chemistry letters. 27(7): 1547-1550. DOI: 10.1016/j.bmcl.2017.02.038
Rudiana T., Fitriyanti., dan Adawiah. 2018. Aktivitas antioksidan dari batang gandaria (Bouea macrophylla Griff). Educhemia Jurnal Kimia dan Pendidikan. 3(2): 195-205. DOI: 10.30870/educhemia.v3i2.3328
Sekhon S. 2012. Antioxidant,antiinflamatory and hypolipidemic properties of apple flavonols [thesis]. Nova scotia: Noca Scotia Agricultural College Truro.
Su Xu, Na Li, Meng-Meng Ning, Cai-Hong Zhou, Qiao-Rong Yang, dan Meng-Wi Wang. 2006. Bioactive Comounds from Peperomia pellucida. J. Nat. Prod. 69: 247-250 . DOI: 10.1021/np050457s
WHO. Raised cholesterol situation and trends [internet]. World Helath Organization. 2018 [sited 15 Juni 2020] availabe from: https://www.who.int/gho/
ncd/risk_factors/cholesterol_text/en/
Zeka K., Ruparelia K., Arroo RRJ., Budriesi R., dan Mucucci M. 2017. Flavonoids and their metabolites: prevention in cardiovascular diseases and diabetes. MDPI. 5(19): 1-18. DOI: 10.3390/diseases5030019
Anggraini DI, dan Nabillah LF. 2018. Activity Test of Suji Leaf Extract (Dracaena angustifolia Roxb.) on In Vitro Cholesterol Lowering. Jurnal Kimia Sains dan Aplikasi. 21(2): 54-58
Asker E., Akin S., dan Hokelek T. 2006. 5,3’-Dihydroxy-3,6,7,4’ tetramethoxyflavone. Acta Crystallographica section E structure Reports Online. 62(9): o4519-o4161. https://doi.org/10.1515/ncrs-2017-0141
Attarde D., Pawar J., Chaudhari B., dan Pal S. 2010. Estimation of sterols content in edible oil and ghee samples. Internationa Journal of Pharmaceutical and Sciences Review and Research. 5(1): 135-137.
Baum JA., Teng H., Erdman JW., Weigel RM., Klein BP., Persky VW., Freels S., Surya P., Bakhit RM., Ramos E., Shay NF., dan Potter SM. 1998. Long-term intake of soy protein improves blood lipid profiles and icrases mononuclear cell low-density-lipoprotein receptor messenger RNA in hypercholesterolemic, postmenopausal women. The American Jurnal of Clinical Nutrituin. 68(3): 541-551. DOI: 10.1093/ajcn/68.3.545
Dalimartha S. 2002. Resep tumbuhan obat untuk menurunkan kolesterol. Jakarta : Penebar swadaya
Dalimarta S. 2006. Atlas Tumbuhan Obat Indonesia Jilid 4. Jakarta: Puspa Swara
Das KC., Farmer WJ., dan Winstein B. 1970. Phytochemical studies IX A new flavone velutin. J. Org. Chem.: 2970 95(11): 3989-3890. DOI: 10.1021/jo00836a101
Dipiro JT. 2005. Pharmacotherapy: A patophysiologic approach sixth edition edition. New York: McGraw-Hill Medical Publishing Division.
Fathoni A, Rudiana T, dan Adawiyah. 2019. Characterization and antioxidant assay of yellow frangipani flower (Plumeria alba) Extract. Jurnal Pendidikan Kimia. 1-7. DOI: 10.24114/jpkim.vlli1.13034
Gini T., dan Jothi J. 2013. Preminary phytochemichal screening of whole plant extracts of Peperomia pellucida (Linn.) HBK. (Piperaceae) and Marsilea quadrifolia Linn. (Marsileaceae). International Journal of Pharmacognosy and Phytochemical Research. 5(3): 200-214
Hadiarti D. 2017. Uji aktivitas ekstrak buas-buas (Premna serratifolia Linn) sebagai antikolesterol secara in vitro. Jurnal Ilmiah Ar-Raz. 5(1): 22-29. DOI: 10.29406/arz.v5i1.644
Hanani E. 2015. Analisis fitokimia. Jakarta: EGC Penerbit buku kedokteran
Januarti IB, Santoso A., dan Razak AS. 2017. Ekstraksi senyawa flavonoid daun jati (Tectona grandis L.) dengan metode ultrasonik (kajian rasio bahan: pelarut dan lama ekstraksi). Media Farmasi Indonesia. 12(2): 1259-1266
Judas N., Kaitner B., dan Mestrovic E. 2006. 1,3-Diphenylpropan-1-one. Acta Crystal-lographica Section E Structure Reports Online. 51(12): 2656-2658
Kang J., Xie C., Li Z., Nagarajan S., Schauss AG., Wu T., dan Wu X. 2011. Flavonoids from acai (Euterpe oleracea Mart.) pulp and their antioxidant and antiinflamatory activities. Food Chem. 128: 152-157. DOI: 10.1016/j/foodchem.2011.03.011
Majumder P., Abraham P., dan Stya V. 2011. Ethno-medicinal, phytochemical and pharmacological review of an amazing medicinal herb Peperomia pellucida (L.) HBK. Reseacrh Journal of Pharmaceutical, Biological, and Chemical. 2(4): 358-364.
Mawati DI. 2017. Uji aktivitas antihiperurisemia ekstrak etil asetat tanaman suruhan (Peperomia pellucida L. Kunth) pada tikus putih jantan yang dienduksi kafein [skripsi]. Jakarta: Program Studi Farmasi UIN Syarif Hidayatullah.
Mayes PA., Murray RK., Granner DK., dan Rodwell VW. 2003. Biokimia Harper ed. 25. Jakarta: Buku Kedokteran.
Mazroatul C., Deni GD., Habibi NA., Saputri GF. 2016. Anti-hypercholesterolemia activity of ethano extract Peperomia pellucida. Alchemy Jurnal Penelitian Kimia. 12(1): 86-94. https://doi.org/ 10.20961/alchemy.12.1.948.88.94
Mursu J., Nurmi T., Tuomainen TP., Ruusunen A., Salonen JT., dan Voutilainen S. 2007. The intake of flavonoids and carotid antheroscierosis: the kuopio ischaemic heart disease risk factory. Br. J. Nutr. 98: 814-818. DOI: 10.1017/S0007114507744410
Nuraeni F. dan Bernadetha S. 2018. Aktivitas antioksidan serta identifikasi senyawa dari ekstrak jamu lingzhi (Ganoderma lucidum) dengan liquid chromatography-mass spectrometry (LC-MS). Seminar Nasional Edusainstek FMIPA UNIMUS. 1-10
Priatna M., Sartika AM., Ambaryani R. 2015. Uji banding aktivitas antikolesterol ekstrak etanol buah pepiro (Solanum muricatum Ait) dan buah strawberry (Fragaria X ananassa Duchesne) pada tikus putih jantan. Jurnal Kesehatan Bakti Tunas Husada. 13(1): 165-172
Reddy MVB, Hung HY, Kuo PC, Huang GJ, Chan YY, Huang SC, Chan YY, Huang SC, Wu SJ, Morris NS, Lee KH, dan Wu TS. 2017. Synthesis and biological evaluation of chalcone, dyhydrochalcone, and 1,3-diarypropane analogs as anti-inflamatory agents. Bioorganic and medicinal chemistry letters. 27(7): 1547-1550. DOI: 10.1016/j.bmcl.2017.02.038
Rudiana T., Fitriyanti., dan Adawiah. 2018. Aktivitas antioksidan dari batang gandaria (Bouea macrophylla Griff). Educhemia Jurnal Kimia dan Pendidikan. 3(2): 195-205. DOI: 10.30870/educhemia.v3i2.3328
Sekhon S. 2012. Antioxidant,antiinflamatory and hypolipidemic properties of apple flavonols [thesis]. Nova scotia: Noca Scotia Agricultural College Truro.
Su Xu, Na Li, Meng-Meng Ning, Cai-Hong Zhou, Qiao-Rong Yang, dan Meng-Wi Wang. 2006. Bioactive Comounds from Peperomia pellucida. J. Nat. Prod. 69: 247-250 . DOI: 10.1021/np050457s
WHO. Raised cholesterol situation and trends [internet]. World Helath Organization. 2018 [sited 15 Juni 2020] availabe from: https://www.who.int/gho/
ncd/risk_factors/cholesterol_text/en/
Zeka K., Ruparelia K., Arroo RRJ., Budriesi R., dan Mucucci M. 2017. Flavonoids and their metabolites: prevention in cardiovascular diseases and diabetes. MDPI. 5(19): 1-18. DOI: 10.3390/diseases5030019
Published
2022-07-31
How to Cite
RUDIANA, Tarso et al.
Profiling Senyawa Metabolit Sekunder Fraksi Aktif Antihiperkolesterolemia Dari Herba Ketumpang Air (Peperomia pellucida L).
Jurnal Farmasi Udayana, [S.l.], p. 26-31, july 2022.
ISSN 2622-4607.
Available at: <https://ojs.unud.ac.id/index.php/jfu/article/view/68549>. Date accessed: 21 nov. 2024.
doi: https://doi.org/10.24843/JFU.2022.v11.i01.p05.
Section
Articles
