Aktivitas Antibakteri Fungi Endofit Daun Sirsak (Annona muricata Linn.) terhadap Vibrio cholerae dan Bacillus subtilis
Abstract
Sirsak plant is traditional plant that believed empirically as cough medicine, dysentery, rheumatism, lowering uric acid levels and diarrhea community. Vibrio cholerae and Bacillus subtilis types bacteria can cause diarrhea. This study aims to determine the number endophytic fungi found from Sirsak leaves, to determine antibacterial activity endophytic fungi isolates from Sirsak leaves against Vibrio cholerae, and Bacillus subtilis. Furthermore, inoculated repeatedly on SDA for 5-7 days until isolates were obtained. Each isolate was fermented with GDP for 2-3 weeks and then extracted with ethyl acetate. Ethyl acetate extract was used antibacterial with agar diffusion method MHA media. Test results showed that 5 isolates were obtained, namely White DS isolate, Toska DS isolate, Green DS isolate, Cream DS isolate, and Blackish Brown DS isolate. Where DS White isolate suspected of Cylindrocladium sp, DS Toska isolate suspected of Aspergillus fumigatus, DS Green, isolate suspected of Aspergillus flavus, DS Cream isolate suspected of Aspergillus terreus, DS Blackish Brown isolate suspected of Aspergillus niger. Sirsak leaf endophytic fungal isolates (White DS, Toska DS, Green DS, Cream DS and Blackish Brown DS) showed antibacterial activity against Vibrio cholerae and Bacillus subtilis.
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References
Fatur Bayjili, M., Chamzurni, T., & Jauharlina, J. (2023). Pengaruh Kerapatan Tanaman Penaung terhadap Tingkat Serangan Hama Penggerek Buah Kopi (Hypothenemus hampei) dan Cendawan Entomopatogen Beauveria bassiana di Perkebunan Kopi Arabika Gayo (The Effect of Shade Tree Density on Coffee Berry Borrer (Hypotenemus . Jurnal Ilmiah Mahasiswa Pertanian, 8(4), 1033–1042.
Febriyossa, A., & Salsabil, A. (2022). Isolasi dan Identifikasi Jamur Aspergillus sp. Pada Paru-paru Ayam Pedaging yang Dijual di Pasar Cengkareng Jakarta Barat. Jurnal Sehat Indonesia (JUSINDO), 4(01), 28–35. https://doi.org/10.36418/jsi.v4i01.43
Hutajulu, E. F., Anna, N., Batara, E., & Siregar, M. (2019). Uji infeksi cylindrocladium sp pada tiga klon hibrid Eucalyptus grandis x Eucalyptus pellita. Peronema Forestry Science Journal, 4(3), 148–158.
Indriani, C., FR, F., & L, K. (2020). Identification Of Aspergillus Sp Growth On White Bread Against Storage Temperature Indriani C, Fadhila FR, Kodariah L. Jurnal Kesehatan Rajawali, 10(2), 92–103. http://ojs.rajawali.ac.id/index.php/JKR/article/view/75
Jamilatun, M., Azzahra, N., & Aminah, A. (2020). Perbandingan Pertumbuhan Aspergillus fumigatus pada Media Instan Modifikasi Carrot Sucrose Agar dan Potato Dextrose Agar. Jurnal Mikologi Indonesia, 4(1), 168–174. https://doi.org/10.46638/jmi.v4i1.69
Jessica Altin Suhardi, Sesilia Rante Pakadang*, Sisilia Teresia Rosmala Dewi, S. R., & Djuniasti Karim, A. M. S. (2023). Isolasi, Identifikasi Dan Aktivitas Antibakteri Dari Fungi Endofit Daun Miana Terhadap Escherichia Coli Dan Vibrio Cholerae. 19(1).
Kusuma, E. W., Djatmiko, D., & Rasyidah, R. (2021). Pandemi dan Pemenuhan Hak Anak: Studi Kasus Peran Unicef di Yaman pada Masa COVID-19. Transformasi Global, 8(2), 189–204. https://doi.org/10.21776/ub.jtg.2021.008.02.7
Lindawati, S., & Rini, C. S. (2019). Identifikasi Aspergillus flavus pada Kue Pia yang Di Jual Di Dusun Warurejo Kabupaten Pasuruan. Medicra (Journal of Medical Laboratory Science/Technology), 2(2), 56–62. https://doi.org/10.21070/medicra.v2i2.1618
Maharani, K. (2018). Uji Antibakteri Ekstrak Kulit Buah Dan Biji Manggis (Garcinia mangostana) Pada Bakteri Penyebab Jerawat (Staphylococcus epidermidis) Dengan Menggunakan Solven Etanol. Universitas Airlangga, 5–26.
Manurung, L. P., & Kurniatuhadi, R. (2022). Inventarisasi Jamur Endofit dari Daun Avicennia marina di Mempawah Mangrove Center , Desa Pasir , Kalimantan Barat Inventory of Endophyte Fungi from Avicennia marina Leaves in Mempawah Mangrove Center , Pasir Village , West Borneo. LenteraBio, 11(3), 378–384.
Murtafi’ah, N.-. (2021). Identifikasi Jamur Aspergillus Sp Pada Roti Tawar Sebelum Masa Kadaluarsa Di Pasar Burungtungku Kota Bandung. Klinikal Sains : Jurnal Analis Kesehatan, 9(2), 122–130. https://doi.org/10.36341/klinikal_sains.v9i2.2159
Nontji, M., Palad, M., & Diniarti, W. (2023). Isolasi dan Inventarisasi Cendawan Endofit pada Tanaman Tomat. Agrium: Jurnal Ilmu Pertanian, 26(1), 37–49. https://doi.org/10.30596/agrium.v26i1.13736
Prasetyo, M. H., & Hasyim. (2022). Nusantara Hasana Journal. Nusantara Hasana Journal, 1(11), 22–32. http://nusantarahasanajournal.com/index.php/nhj/article/view/279
Putra, G. W., Ramona, Y., & Proborini, M. W. (2020). Eksplorasi Dan Identifikasi Mikroba Pada Rhizosfer Tanaman Stroberi (Fragaria x ananassa Dutch.) Di Kawasan Pancasari Bedugul. Metamorfosa: Journal of Biological Sciences, 7(2), 62. https://doi.org/10.24843/metamorfosa.2020.v07.i02.p09
Putri, F. S., Rahayu, T. P., & Fitriyati, L. (2023). Antibacterial Activity Of Extract Ethanol White Turmeric ( Curcuma mangga Val ) Test On Bacillus subtilis Causes Diarrhea Uji Aktivitas Anti Bakteri Ekstrak Etanol Kunir Putih ( Curcuma mangga Val ) Terhadap Bakteri Bacillus subtilis Penyebab Diare. Prosiding University Research Colloquium, 622–634.
Septiana, L. M., Ajizah, A., & Halang, B. (2023). Karakterisasi Jamur Mikroskopis Pada Buah Naga Merah (Hylocereus polyrhizus) Sebagai Materi Pengayaan Konsep Fungi Kelas X SMA/MA. JUPEIS : Jurnal Pendidikan Dan Ilmu Sosial, 2(3), 24–32. https://doi.org/10.57218/jupeis.vol2.iss3.621
Urip, U., Jiwintarum, Y., & Gandi, N. L. P. G. (2021). Studi Jamur Aspergillus fumigatus di Pasar Cakranegara Kota Mataram Penyebab Penyakit Aspergillosis Menggunakan Media Pertumbuhan Potato Dextrose Agar. Bioscientist : Jurnal Ilmiah Biologi, 9(2), 631. https://doi.org/10.33394/bioscientist.v9i2.4560
