UJI AKTIVITAS ANTIINFLAMASI EXSTRAK ETANOL RIMPANG ANDONG MERAH (Cordyline fruticosa (L.) A Chev) PADA UDEMA KAKI TIKUS WISTAR YANG DIINDUKSI KARAGENAN
Abstract
Rimpang andong merah (Cordyline fruticosa) ialah tumbuhan obat yang mengandung saponin yang dikenal selaku antiinflamasi. Tujuan riset ini adalah menguji aktivitas antiinflamasi ekstrak etanol rimpang andong merah. Analisis fitokimia dilakukan secara kualitatif dengan uji fitokimia menggunakan reagen pereaksi warna sedangkan uji aktivitas antiinflamasi dengan menggunakan 25 ekor tikus Wistar yang diinduksi dengan karagenan kemudian dikelompokkan menjadi 5 yaitu; (P1) kontrol negatif, (P2) kontrol positif, serta (P3, P4, dan P5) kelompok ekstrak uji dengan dosis 125, 250, dan 500 mg/kg BB. Hasil uji fitokimia menunjukkan ekstrak tersebut terdapat senyawa golongan alkaloid, steroid, fenolik, flavonoid, dan saponin. Dari hasil uji aktivitas antiinflamasi, pemberian ekstrak dosis 125 mg/kg BB memberikan hambatan inflamasi sebesar 65,876% sebaliknya dosis 250 mg/kg BB dapat menghambat inflamasi sebesar 59,994% dan dosis 500 mg/kg BB memberikan hambatan inflamasi sebesar 73,908% selama 360 menit pengamatan. Hasil uji analisis probit memberikan nilai ED50 sebesar 158,48 mg/kg BB.
Kata kunci: aktivitas antiinflamasi, andong merah, ekstrak, rimpang.
ABSTRACT
Rhizome of red andong (Cordyline fruticosa (L.) A. Chev) is a medicinal plant that contains saponins known to be anti-inflammatory. The research aimed to assess the anti-inflammatory activity of the rhizome ethanol extract of red andong. Phytochemical analysis was done qualitatively by using phytochemical reagents. Anti-inflammatory activity was evaluated using 25 Sprague Dawley male rats which had been divided into five groups: negative control (P1), positive control (P2), and group P3, P4, and P5 given extract at doses of 125; 250; and 500 mg/kgBW, respectively. A phytochemical study revealed that the rhizome ethanol extract consisted of alkaloids, steroids, phenolic, flavonoids and saponins compounds. The anti-inflammatory activity test showed that the administration of the extract at a dose of 125 mg/kgBW resulted in an inflammatory inhibition of using 65,876%, on the other hand, a dose of 250 mg/kgBW could inhibit inflammation by 59,994%, and a dose of 500 mg/kgBW had the inflammatory inhibition of 3,908% for 360 minutes of observation. The results of the probit analysis gave an ED50 value of 158,48 mg/kgBW.
Keywords: anti-inflammatory activity, extract, rhizome, red andong.
Downloads
References
Bogoriani, N. W., Suaniti, N. M., Putra, A. A. B., and Pradnya, L. K. D. 2019. The activity Cordyline Terminalis leaf extract as antidiabetic in obese wistar rats. InternationallJournalloffPharmaceuticall Research&Allied Sciences. 8(2):206-213.
Bogoriani, N. W., Laksmiwati, A. A. I. M., Putra, A. A. B, Heltyani, W.E., and Lestari, K. D. P. 2019. Saponin role of andong leaf as Antiobesty in rats. International Journal of Pharmaceutical Research. 11(2):382-389.
Bogoriani, N. W., Manuaba, I. B. P., Suastika, K., and Wita, I. W. 2015. Cordyline terminalis Kunth Leave’s saponin lowered plasma cholesterol and bile acids levels by increased the excretion of fecal total bile acids and cholesterol in male wistar rats. European Journal of Biomedical and Pharmaceutical Sciences. 2(5):122-134.
Bogoriani, N. W., and Ariati, N. K. 2018. The activity of Bali andong rhizome extract of Cordyline terminalis Kunth as hypolipidemia agent in Wistar rats with High - Cholesterol diet. International Journal of Pharmaceutical and Phytopharmacological Research. 8(1):75-80.
Chi, Y. S., Lim, H., Park, H., and Kim, H. P. 2003. Effect of wogonin, a Plant flavonones from Scutelaria radix on Skin Inflammation in vivo Regulation on Inflammation asociated Gene Expression. Biochem Pharmacol. 66:1271-1278.
Hazanah, N. A., Fikri, H., Elin, F., dan Zuhrotun, A. 2011. Analisis Kandungan Minyak Atsiri dan Uji Aktivitas Antiinflamasi Ekstrak Rimpang Kencur (Kaempferia galanga L.). Fakultas Farmasi, Universitas Padjadjaran. Bandung.
Katzung, B. G. 2002. Farmakologi Dasar dan Klinik. Salemba. Jakarta.
Kil, T. G., Kang, S. H., Kim, T. H., Shin, K. C., Kun, D. O. 2019. Emzymatic Biotransformation of Ballon Flower Root Saponins into Bioactive Platycodin D by Deglucosylation with Caldicellulosirupto bescii - Glucosidase. International Journal of Molecular Sciences. 20:38-54.
Murphy, J. E., Robert, C., and Kupper, T. S. 2000. Interleukin-1 and cutaneous inflammation: a crucial link between innate and acquired immunity. J. Invest Drematoli. 114:602-608.
Mycek, M .J., Harvey, R. A., dan Champe, P. C. 2001. Farmakologi Ulasan Bergambar 2nd ed. Jakarta:.H. Hartanto Ed.
Olson, K. R., Anderson, I. B., Benowitz, N. L., Blanc, P. D., Clark, R. F., Kearney, T. E., Kim-Katz, S. Y., and Wu, A. H. B. 2012. Poioning & Drug Overdose, 6th edition. United States: McGraw-Hill Companies, Inc.
Price, S. A., and Wilson, L. M. 2000. Patofisiologi, konsep klinis proses-proses penyakit, Edisi 6. Jakarta: Penerbit buku kedokteran EGC. 57-58.
Rostagno, M. A., Villares, A., Guillamon, E., Lafuente, A. G., and Martinez, J. A. 2009. Sample Preparation for the Analysis of Isoflavones from Soybeans and Soy foods. Journal of Chromatography A. 1216920090:2-29.
Wijaya, L., Saleh, I., Theodorus dan Salni. 2015. Efek Antiinflamasi Fraksi Daun Andong (Cordyline fruticosa L) PADA Tikus Putih Jantan (Rattus novergicus) galur Sprague Dawley Biomedical Journal of Indonesia. 1(1):16-24.
This work is licensed under a Creative Commons Attribution 4.0 International License
