ANTIOXIDANT AND INHIBITION OF ALPHA-AMYLASE ACTIVITIES OF PAPASAN (Coccinia grandis (L.) Voigt) LEAVES AND BELIMBING WULUH (Averrhoa bilimbi L.) FRUITS COMBINED INFUSIONS
Abstract
The pharmacology activity of an herb can be increased by combining it with other herbs. This study aimed to make herbal infusions from the combination of papasan (Coccinia grandis (L.) Voigt) leaves and belimbing wuluh (Averrhoa bilimbi L.) fruits. Antioxidant and inhibition properties of alpha-amylase enzymes were then tested. A mixture of Coccinia grandis (L.) Voigt leave and Averrhoa bilimbi L. fruit powder with a mass ratio of 1:3, 1:1, and 3:1 was brewed with 100 mL of boiling water for 5 minutes. The mixture was then filtered and allowed to cool. The antioxidant activity test was carried out using 2,2-diphenyl-1-picrylhydrazyl (DPPH) as an oxidant source and the alpha-amylase activity inhibition test was performed using the starch-iodine method. The results of the antioxidant activity test using the DPPH method showed that samples with a 1:1 combination ratio had the lowest IC50 value (2.56±0.29 mg/ml) which indicated the strongest radical scavenging activity. The highest inhibition of alpha-amylase activity was found in the sample with a ratio of 3:1 (IC50 = 0.75±0.45 mg/mL). However, in general all the combination ratios of Coccinia grandis (L.) Voigt leaves and Averrhoa bilimbi L. fruits had good potential as healthy beverages.
Keywords: herbal combination, healthy beverages, antioxidant, inhibition of alpha-amylase
Aktivitas famakologi suatu herbal dapat ditingkatkan dengan menggabungkannya dengan herbal lain. Penelitian ini bertujuan untuk membuat infusa herbal dari kombinasi daun papasan (Coccinia grandis (L.) Voigt) dan buah belimbing wuluh (Averrhoa bilimbi L.). Sifat antioksidan dan penghambatan enzim alfa-amilase infusa tersebut kemudian diuji. Campuran serbuk daun papasan dan buah belimbing wuluh dengan perbandingan massa 1:3, 1:1, dan 3:1 diseduh dengan 100 mL air mendidih selama 5 menit. Campuran tersebut kemudian disaring dan dibiarkan dingin. Uji aktivitas antioksidan dilakukan dengan menggunakan 2,2-difenil-1-pikrilhidrazil (DPPH) sebagai sumber oksidan dan uji penghambatan aktivitas alfa-amilase dilakukan dengan menggunakan metode pati-iodin. Hasil uji aktivitas antioksidan menggunakan metode DPPH menunjukkan bahwa sampel dengan kombinasi rasio 1:1 memiliki nilai IC50 terendah (2,56±0,29 mg/mL) yang mengindikasikan aktivitas penangkapan radikal terkuat. Penghambatan aktivitas alfa-amilase tertinggi ditemukan pada sampel dengan rasio 3:1 (IC50 = 0,75±0,45 mg/mL). Namun, secara umum semua kombinasi rasio daun papasan dan buah belimbing wuluh memiliki potensi yang baik sebagai minuman sehat.
Kata kunci: kombinasi herbal, minuman kesehatan, antioksidan, penghambatan alfa-amilase
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References
Attanayake, A. P., Jayatilaka, K. A. P. W., Mudduwa, L. K. B., and Pathirana, C. 2016. In vivoantihyperlipidemic, antioxidative effects of cocciniagrandis (L.) Voigt (cucurbitaceae) leaf extract: an approach to scrutinize the therapeutic benefits of traditional Sri Lankan medicines against diabetic complications. International Journal of Pharmaceutical Sciences and Research. 7(10): 3949-3958.
Attanayake, A. P., Jayatilaka, K. A. P. W., Pathirana, C., andMudduwa, L. K. B. 2013. Efficacy and toxicological evaluation of Cocciniagrandis (Cucurbitaceae) extract in male Wistar rats. Asian Pacific Journal of Tropical Disease. 3(6): 460-466.
Attanayake, A. P., Jayatilaka, K. A. P. W., Pathirana, C., and Mudduwa, L. K. B. 2015. Antihyperglycemic activity of Cocciniagrandis (L.) Voigt in streptozotocin induced diabetic rats. Indian Journal of Traditional Knowledge. 14(3): 376-381.
Arumugam, G., Manjula, P., and Paari, N. 2013. A review: Anti diabetic medicinal plants used for diabetes mellitus. Journal of Acute Disease. 2(3):196-200.
Chawla, R., Thakur, P., Chowdhry, A., Jaiswal, S., Sharma, A., Goel, R., et al. 2013. Evidence based herbal drug standardization approach in coping with challenges of holistic management of diabetes: a dreadful lifestyle disorder of 21st century. Journal of Diabetes & Metabolic Disorders. 12(1): 35.
Gómez-Huelgas, R., Gómez Peralta, F., RodríguezMañas, L., Formiga, F., PuigDomingo, M., Mediavilla Bravo, J. J.2018. Treatment of type 2 diabetes mellitus in elderly patients. RevistaClínica Española. English Edition. 218(2): 74-88.
Jain, D. P., Pancholi, S. S., Patel, R. 2011. Synergistic antioxidant activity of green tea with some herbs. Journal of Advanced Pharmaceutical Technology and Research. 2(3): 177-183.
Jemaa, H. B., Jemia, A. B., Khlifi, S., Ahmed, H. B., Slama, F. B., Benzarti, A., et al. 2017. Antioxidant activity and α-amylase inhibitory potential of Rosa canina L. African Journal of Traditional, Complementary and Alternative Medicines.14(2): 1-8.
Kazeem, M. I., Adamson, J. O., and Ogunwande, I. A. 2013. Modes of inhibition of α-amylase and α-glucosidase by aqueous extract of MorindalucidaBenth Leaf. BioMed Research International. 2013: 1-6.
Kurup, S. B. and Mini, S. 2017.Averrhoabilimbi fruits attenuate hyperglycemia-mediated oxidative stress in streptozotocin-induced diabetic rats. Journal of Food and Drug Analysis. 25(2): 360-368.
Lau, K-M., Lai, K-K., Liu, C-L., Tama, J. C-W., To, M-H., Kwok, H-F., et al. 2012. Synergistic interaction between Astragali radix and Rehmanniae radix in a Chinese herbal formula to promote diabetic wound healing. Journal of Ethnopharmacology. 141: 250-256.
Marín-Peñalver, J. J., Martín-Timón, I., Sevillano-Collantes, C., and delCañizo-Gómez, F. J. 2016. Update on the treatment of type 2 diabetes mellitus. World Journal of Diabetes. 7(17): 354-95.
Meenatchi, P., Purushothaman, A., and Maneemegalai, S. 2017. Antioxidant, antiglycation and insulinotrophic properties of Cocciniagrandis (L.) in vitro: Possible role in prevention of diabetic complications. Journal of Traditional and Complementary Medicine. 7(1): 54-64.
Mohammed, S. I., Chopda, M. Z., Patil, R. H., Vishwakarma, K. S., andMaheshwari, V. L. 2016. In vivo antidiabetic and antioxidant activities of Cocciniagrandis leaf extract against streptozotocin induced diabetes in experimental rats. Asian Pacific Journal of Tropical Disease. 6(4):298-304.
Neagu, A., Radu, G. L., Albu, C., andPaun, G. 2018. Antioxidant activity, acetylcholinesterase and tyrosinase inhibitory potential of Pulmonariaofficinalis and Centariumumbellatum extracts. Saudi Journal of Biological Sciences. 25(3): 578-585.
Nedamani, E. R, Mahoonak, A. S., Ghorbani, M., and Kashaninejad M. 2015. Evaluation of antioxidant interactions in combined extracts of green tea (Camellia sinensis), rosemary (Rosmarinusofficinalis) and oak fruit (Quercusbranti). Journal of Food Science Technology. 52(7): 4565-4571.
Okokon, J. E., Umoh, U. F., Ekpo, B. A. J., and Etim, E. I. 2013.Antidiabetic study of combined extracts of Vernoniaamygdalina, Ocimumgratissimum, and Gongronemalatifolium on alloxan-induced diabetic rats. Journal of Natural Pharmaceuticals. 4(1): 28-31.
Rahman, M. M., Habib, M. R., Hasan, M. A., Al Amin, M., Saha, A., and Mannan, A. 2014. Comparative assessment on in vitro antioxidant activities of ethanol extracts of Averrhoabilimbi, Gymnemasylvestre and Capsicum frutescens. Pharmacognosy Research. 6(1): 36-41.
Rodrigues, M. J., Oliveira, M., Neves, V., Ovelheiroa, A., Pereiraa, C. A., Neng, N. R., et al. 2019. Coupling sea lavender (LimoniumalgarvenseErben) and green tea (Camellia sinensis (L.) Kuntze) to produce an innovative herbal beverage with enhanced enzymatic inhibitory properties. South African Journal of Botany. 120: 87-94.
Sing, S., Usman, K., and Banerjee, M. 2016.Pharmacogenetic studies update in type 2 diabetes mellitus. World Journal of Diabetes. 7(15): 302-315.
Suluvoy, J. K., Sakthivel, K. M., Guruvayoorappan, C., and Grace, B. V. M. 2017. Protective effect of Averrhoabilimbi L. fruit extract on ulcerative colitis in wistar rats via regulation of inflammatory mediators and cytokines. Biomedicine & Pharmacotherapy. 91: 1113-1121.
Tuyen, P. T., Khang, D. T., Minh, L. T., Minh, T. N., Ha, P. T. T., Elzaawely, et al. 2016; Phenolic Compounds and Antioxidant Activity of Castanopsisphuthoensis and Castanopsisgrandicicatricata. International Letters of Natural Sciences. 55: 77-87.
Wardani, N. A. K. 2017. Enzim α-amilase inhibitor padaekstrak air kacangmerah (Phaseolus vulgaris L.) untukpenanggulangan diabetes melitus. JurnalIlmuPangandanHasilPertanian. 1(2): 50-59.
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