ISOLASI DAN IDENTIFIKASI SENYAWA GOLONGAN TRITERPENOID DARI EKSTRAK n- EKSANA DAUN KEPUH (Sterculia foetida L.) SERTA UJI AKTIVITAS ANTIRADIKAL BEBAS

Abstract

Kepuh leaves can be used as traditional medicine to cure various diseases. Maseration of ± 1000 g dry
powder of Kepuh leaves with n-hexane as the solvent resulted in 2,10 g n-hexane concentrated extract. Separation of
this extract with column chromatography gained two yellow amorphous crystal isolates of F3.1 and F3.2 with 0,0195 g
and 0,0032 g in weight, respectively.
The result of phytochemical test of isolate F3.1 with Liebermann-Burchard reagent showed that the isolate
positively contained triterpenoid. Identification of isolate F3.1 with spectrophotometer UV-Vis showed one broad
peak at 265 nm. The transition occured probably was n *, that was caused by C=O chromophor. Infrared
spectrum showed characteristic groups of OH at 3294,42 cm-1, aliphatic CH at 2924,09 cm-1 and 2854,65 cm-1, C=O
at 1743,65 cm-1, bending CH2 and bending CH3 at 1458,18 cm-1 and 1381,03 cm-1 and alcoholic CO at 1172,72 cm-1.
Free antiradical activity test against isolate F3.1 was carried out by spectroscopy UV-Vis method. Isolate F3.1 had
characteristic as a free antiradical with DPPH damping percentage at the 5th minute was 85,33% and at the 60th
minute was 88,52%.
According to phytochemical tests and spectrophotometry analysis, isolates from kepuh leaves was expected
to contain true triterpenoid group compounds with free antiradical activity OH group, aliphatic CH, C=O and
alcoholic C-O as the functional groups.