ISOLASI, IDENTIFIKASI, DAN UJI AKTIVITAS ANTIBAKTERI SENYAWA GOLONGAN TRITERPENOID PADA RIMPANG TEMU PUTIH (Curcuma zedoaria (Berg.) Roscoe)

  • Wiwik Susanah Rita

Abstract

Isolation, identification, and antibacterial activity examination of triterpenoid compounds from rhizome of
Curcuma zedoaria (Berg.) Roscoe have been conducted. Maseration of 600 g dry powder of those rhizome using nhexane
and ethanol respectively yielded 9,79 g of n-hexane extract and 23,45 g of ethanol extract. The ethanol
extract containing triterpenoids was dissolved into ethanol-water (7:3) mixture. The mixture was evaporated to
remove the ethanol and then partitioned by chloroform. Hence, two extract were produced i.e., chloroform and water
extract. The n-hexane and chloroform extracts obtained contained triterpenoids based on fitochemical test of
Lieberman-Burchard. Antibacterial test showed that only chloroform extract was active with inhibition zone of 2 mm
for Staphylococcus aureus.
Compound separation of the chloroform extract using column chromatography produced 0,44 g brown
isolate which contained triterpenoids (fraction F1). The isolate inhibited the growth of Staphylococcus aureus and
Escherichia coli with weak inhibition zone at 500 ppm and 1000 ppm. Infrared spectra indicated that the isolate was
a carboxylic acids triterpenoids, with characteristic functional groups of –OH bonded, –CH, C=O of carboxylic acids,
–C=C, –CH2, –CH3, and C–O alcohol. The ultraviolet-visible spectra showed maximum absorption at 242 nm and
280 nm.

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Author Biography

Wiwik Susanah Rita
Jurusan Kimia FMIPA Universitas Udayana, Bukit Jimbaran
How to Cite
SUSANAH RITA, Wiwik. ISOLASI, IDENTIFIKASI, DAN UJI AKTIVITAS ANTIBAKTERI SENYAWA GOLONGAN TRITERPENOID PADA RIMPANG TEMU PUTIH (Curcuma zedoaria (Berg.) Roscoe). Jurnal Kimia (Journal of Chemistry), [S.l.], nov. 2012. ISSN 2599-2740. Available at: <https://ojs.unud.ac.id/index.php/jchem/article/view/2771>. Date accessed: 24 jan. 2022.
Section
Articles

Keywords

Curcuma zedoaria (Berg.) Roscoe, triterpenoids, Staphylococcus aureus, Escherichia coli

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