PENELUSURAN SENYAWA SITOTOKSIK PADA KULIT BIJI NYAMPLUNG (Calophyllum inophyllum L.) DAN KEMUNGKINAN KORELASINYA SEBAGAI ANTIKANKER

Abstract

Isolation and identification of active toxic compounds from nyamplung skin bean (Calophyllum inophyllum
L). has been carried out. As much as 12,43 g of concentrated methanol extract was resulted from 1.0 kg dry powder
nyamplung skin bean that was macerated using methanol. This extract was dissolved into methanol-water (7:3) and
then partitioned with n-hexane and chloroform respectively. The result of toxicity test to both extract showed that
chloroform extract was the most active toxic with LC50 = 239,88 ppm
Separation of chloroform extract using preparative thin layer chromatography (TLC) resulted in F5 fractions
that was white yellowish as much as 52 mg and LC50 = 151,36 ppm. Infra red and UV-vis spectroscopy were
employed in order to identify the F5 fraction. The result of identification showed that isolate was aldehide
triterpenoide compounds which had functional group like –OH (3429 cm-1), -CH aldehyde (2922,9 and 2852,9 cm-1),
-CºC- (2360 cm-1), -C=O (1742,7 cm-1), -C=C- alkene(1637,0 cm-1), -CH3, -CH2 (1542,9, 1480,7, 1401,9 cm-1), -CO-
(1090 cm-1), It showed absorbed at broad peaks at l(nm) (CHCl3): 254, 282, and 342,7.