ISOLASI DAN IDENTIFIKASI GOLONGAN SENYAWA TOKSIK DARI DAGING BUAH PARE (Momordica charantia L.)

Abstract

Isolation and identification of toxic compounds were conducted to snake fruit (Momordica charantia L.).
Maseration of 600 grams dried fruit in methanol resulted in 54.250 grams dark green extract which were toxic to
brine shrimp (Artemia salina Leach) with an LC50 = 74.99 ppm. When the extract was dissolved in water and
partitioned using n-hexane and chloroform three extracts were obtained. The n-hexane extract was found to be the
most toxic to brine shrimp (LC50 = 130.439 ppm).
Separation of the n-hexane extract using column chromatography resulted in 7 fractions. The most toxic
fraction (F), LC50 = 33.83 ppm, was pure based on TLC.
FTIR analyses showed that the active components of the extract contains triterpenoides characterized by CH
aliphatic groups (CH2, CH3), binded O-H, C-O, C=C, and C=O. UV-Vis spectra showed absorption at 274,2 and
432,8 nm suggesting an n * electronic transition.