ISOLATION AND IDENTIFICATION MOMORDICIN I FROM LEAVES EXTRACT OF Momordica charantia L.

Abstract

Momordicin I has been isolated from dichloromethane extract of leaves Momordica charantia, L. The
isolation was done by fractionation the dichloromethane extract followed by bioassay using artemia salina,L larvae.
The crude extract was fractionated through reverse phase column chromatography (C18 Sep-Pak) with eluotropic
series solution of MeOH: H2O (0-100% MeOH). The most active fraction, F9 (80% MeOH-20% H2O) was purified
by recrystallisation from chloroform to yield a white crystal melted at 125-128oC. The structure of the active
compound was determined by interpretation of the spectral data (IR, 1HNMR, 13CNMR, DEPT, HCOSY, HMCOSY,
HMBC and MS) and its structure was elucidated as 3,7,23-trihydroxycucurbitan-5,24-dien-19-al which is known as
momordicin I.