KARAKTERISASI TOXOPLASMA GONDII ISOLAT INDONESIA

Abstract

Cathodic protection basically reduces the corrosion rate of a metallic structure by reducing its corrosion
potential, Toxoplasma gondii isolated from diaphragm of sheep at an abbatoir in Sukabumi, West Java had been
characterized by Centre Research Institute for Animal Sciences. The characterization included study of morphology
by optical microscope, study of ultrastructure by transmission electron microscope, the study of the parasite growth
in mice Mus musculus, and study of proteins of the parasite. The growth of parasite in mice had been studied using
two groups of mice i.e., normal group and immunosuppressed group. The number of parasites was compared
statistically using student’ t-pair test. Results showed that dexamethasone at a dose of 5.2 mg/20 g body weight intra
peritoneally to the immunosuppresed mice did not increase the number of extracelluler parasites in the peritoneal
fluid. The best parasite harvest time was on the 4th day after inoculation. Determination of parasite protein obtained
at 4 days after inoculation using sodium dodecyl sulphate polyacrylamide gel electrophoresis showed a dominant
surface protein of 42 kDa.