IDENTIFIKASI SENYAWA AKTIF ANTIMAKAN BIJI SIRSAK (Annona muricata L.) TERHADAP ULAT KUBIS (Plutella xylostella)

Abstract

Identification of antifeedant active compounds from Soursop seeds (Annona muricata Linn) as natural pesticides against Plutella xylostella has been carried out. 1800 g Soursop seed powder  maceration was applied using methanol and n-hexane resulted in 46.42 g extract of methanol and 94.19 g extract of n-hexane. Viscous extract of methanol was diluted with the mixture of methanol:water (7:3), then evaporated until the entire methanol was removed, and after that it was partitioned with chloroform. The antifeedant activity of viscous extract of n-hexane, chloroform and water was tested which showed that the extract of n-hexane had highest activity compared to the others. Separation of 2 g viscous n-hexane extract with Coloumn Chromatography using Gel Silica 60 and n-hexane:aceton (4:1) as the mobile phase resulted in 5 fractions. The fractions were tested against Plutella xyllostella. The results showed that fraction C had the highest antifeedant activity. The isolate of fraction C was then identified using Gas Chromatography-Mass Spectrometry (GC-MS) based on the data base (Database\NISTO2.L and Database\wiley7n.1) suggested the active compounds were ethyl hexadecanoate ester and 11-octadecanoic acid.