Phytochemical Screening and Acute Toxicity Test of Caulerpa lentillifera J. Agardh Infusion Extract on Mice (Mus musculus L.)
Abstract
Caulerpa lentillifera has ingredients that are beneficial for health, such as anti-cancer, antioxidant, anti-diabetic, and immunostimulatory effects. This study was aimed to determine the content of phytochemical compounds and the LD50 acute toxicity dose of C. lentillifera infusion extracts on mice for their utilization as food or medicine. The phytochemical screening was done with a qualitative method. Twenty-five mice have been used for the acute toxicity test and divided into five groups of treatment (Control; 375 mg/kg BW; 750 mg/kg BW; 1.500 mg/kg BW; 3.000 mg/kg BW C. lentillifera infusion extracts orally). Symptoms of toxicity and mortality were observed 24 hours after treatment, and body weight was observed for seven days. The phytochemical screening result showed that the C. lentillifera infusion extracts contained alkaloids, saponins, and tannins. Acute toxicity tests showed no mortality in all treatment groups. Thus, the LD50 value could not be obtained and was classified as non-toxic.
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References
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Anggraito, Y.U., R. Susanti, Retno, S.I., Ari, Y., Lisdiana, Nugrahaningsih, W.H., Noor, A.H., dan Siti, H.B. (2018). Metabolit Sekunder dari Tanaman: Aplikasi dan Produksi. Semarang: Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Semarang.
Badan Pengawas Obat dan Makanan. (2022). Pedoman Uji Toksisitas Praklinik secara in vivo. Peraturan Badan Pengawas Obat dan Makanan Nomor 10 Tahun 2022
Chusniasih, D., dan Tutik. (2020). Uji Toksisitas Dengan Metode Brine Shrimp Lethality Test (BSLT) dan Identifikasi Komponen Fitokimia Ekstrak Aseton Kulit Buah Kakao (Theobroma cacao L.). Analit: Analytical and Environmental Chemistry. Vol. 5(2). https://doi.org/10.23960/aec.v5.i2.2020.p192-201
Febriyanti, R., Muladi, P.M., dan Rahmat, A. (2021). Skrining Fitokimia pada Ekstrak Hasil Proses Infundasi Akar Bajakah. Laporan Penelitian. Prodi DIII Farmasi Politeknik Harapan Bersama.
Fitria, L., Tika, C. D., Kholidatus, S., Mulyati, Rahadian, Y. H., Slamet, W., dan Eko, A. S. (2022). Toksisitas Oral Akut Arthrospira maxima dan Chlorella vulgaris Isolat Glagah pada Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar. AL-KAUNIYAH: Jurnal Biologi. Vol. 15(2): 209-219. https://doi.org/10.15408/kauniyah.v15i2.18716
Julianto, T.S. (2019). Fitokimia Tinjauan Metabolit Sekunder dan Skrining Fitokimia. Yogyakarta: Universitas Islam Indonesia.
Khairuddin, K., Sabri, S., Luqiang, H., dan Zwe-Ling, K. (2020). Caulerpa lentillifera polysaccharides-rich extract reduces oxidative stress and proinflammatory cytokines levels associated with male reproductive functions in diabetic mice. Applied Sciences (Switzerland), 10(24), 1–14. https://doi.org/10.3390/APP10248768
Krisna, D., Patricius, K. A., dan Ines, S. A. (2022). Efek Pemberian Sari Buah Berenuk (Crescentia cujete L.) Terhadap Berat Mencit Galur Swiss-Webster (Mus musculus). Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati, 108–120. https://doi.org/10.24002/biota.v7i2.5255
Maeda, R., Tomoaki, I., Hideshi, I., dan Tatsuji, S. (2012). Induction of apoptosis in MCF-7 cells by β-1,3-xylooligosaccharides prepared from Caulerpa lentillifera. Bioscience, Biotechnology and Biochemistry, 76(5), 1032–1034. https://doi.org/10.1271/BBB.120016
Martha, R.D. dan Atiqoh, Z. (2018). Pengujian Fitokimia dan Penentuan Parameter Kinetik Enzim dengan Penambahan Ekstrak Infusa Air Seledri. Jurnal Wiyata, 5(2), 106–111. http://dx.doi.org/10.56710/wiyata.v5i2.233
Mukhlisa, R., Liza, P., dan Hadi, K. (2021). Uji Fitokimia Ekstrak Infusa Kulit Pisang (Musa acuminata x Musa balbisiana). Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN. Vol. 5(1).
Novindriani, D., Bambang, W., Mohammad, A. (2013). Uji Efek Sedatif Infusa Daun Kratom (Mitragyna speciosa) pada Mencit Jantan Galur BALB/c. Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN. Vol. 3, No. 1.
Oktavia, F.D., dan Suyatno, S. (2021). Skrining Fitokimia, Kandungan Flavonoid Total, dan Aktivitas Antioksidan Ekstrak Etanol Tumbuhan Selaginella doederleinii. Jurnal Kimia Riset. Vol. 6, No.2, 141–153. https://doi.org/10.20473/jkr.v6i2.30904
Stevani, H. (2016). Praktikum Farmakologi. Jakarta: Pusdik SDM Kesehatan.
Sun, Y., Liu, Y., Ai, C., Song, S., & Chen, X. (2019). Caulerpa lentillifera polysaccharides enhance the immunostimulatory activity in immunosuppressed mice in correlation with modulating gut microbiota. Food Funct., 10(7), 4315–4329. https://doi.org/10.1039/C9FO00713J
Tian, H., Liu, H., Song, W., Zhu, L., & Yin, X. (2021). Caulerpa lentillifera polysaccharides enhance the immunostimulatory activity in immunosuppressed mice in correlation with modulating gut microbiota. Natural Product Research, 35(20), 3417–3425. https://doi.org/10.1080/14786419.2019.1700507
Ubang, F., Siregar, V. O., & Herman, H. (2022). Efek Toksik Pemberian Ekstrak Etanol Daun Mekai (Albertisia papuana Becc.) Terhadap Mencit. Proceeding of Mulawarman Pharmaceuticals Conferences, 16, 49–57. https://doi.org/10.25026/mpc.v16i1.672
Wirawan, I. G. P., Dewi, N. K. E. S., Sasadara, M. M. V., Sunyamurthi, I. G. N. A., Jawi, I. M., Wijaya, I. N., Darmawati, I. A. P., Suada, I. K., & Krisnandika, A. A. K. (2022). Phytochemical Analysis and Molecular Identification of Green Macroalgae Caulerpa spp. from Bali, Indonesia. Molecules, 27(15). https://doi.org/10.3390/molecules27154879
Yap, W. F., Tay, V., Tan, S. H., Yow, Y. Y., & Chew, J. (2019). Decoding Antioxidant and Antibacterial Potentials of Malaysian Green Seaweeds: Caulerpa racemosa and Caulerpa lentillifera. Antibiotics, 8(3). https://doi.org/10.3390/antibiotics8030152
You, Y., Song, H., Wang, L., Peng, H., Sun, Y., Ai, C., Wen, C., Zhu, B., & Song, S. (2022). Structural characterization and SARS-CoV-2 inhibitory activity of a sulfated polysaccharide from Caulerpa lentillifera. Carbohydrate Polymers, 280, 119006. https://doi.org/10.1016/J.CARBPOL.2021.119006
Yunedi, A. 2022. Uji Toksisitas Akut Fraksi Daun Sungkai (Peronema canescens Jack.) serta Penentuan Nilai LD50 pada Mencit Putih Jantan. Skripsi. Fakultas Farmasi Universitas Andalas Padang.
Published
2024-04-30
How to Cite
ADIYANTI, Sekar; PHABIOLA, Trisna Agung; SUTARI, Ni Wayan Sri.
Phytochemical Screening and Acute Toxicity Test of Caulerpa lentillifera J. Agardh Infusion Extract on Mice (Mus musculus L.).
International Journal of Biosciences and Biotechnology, [S.l.], v. 11, n. 2, p. 18-24, apr. 2024.
ISSN 2655-9994.
Available at: <https://ojs.unud.ac.id/index.php/jbb/article/view/99271>. Date accessed: 03 dec. 2024.
doi: https://doi.org/10.24843/IJBB.2024.v11.i02.p03.
Section
Articles
