CD4 COUNT FROM CRYOPRESERVATION OF BUFFY COAT AND PBMC

  • Rasmaya Niruri Dept. of Pharmacy, Faculty of Math and Sciences of Udayana University
  • Inna Narayani Dept. of Biology, Faculty of Math and Sciences of Udayana University
  • Wayan Tunas Artama Faculty of Veterinary of Gajah Mada University
  • Mantik Astawa Faculty of Veterinary of Udayana University
  • Ahmad Hamim Sadewa Faculty of Medicine of Gajah Mada University

Abstract

This study aimed to determine CD4 count from cryopreservation of Buffy coat (BC) and PeripheralBlood Mononuclear Cell (PBMC) with and without ficoll. Fifteen EDTA Blood sample (2 ml for eachtube) were drawn from one adult healthy subject. The samples were categorized into five group beforemeasuring the CD4 level (which were fresh whole blood [Group(G)-I], BC without ficoll [fresh <GII>and frozen <G-III>] , and PBMC resulted from BC and ficoll isolation [fresh <G-IV> andfrozen <G-V>]. Each group was replicated three times. Blood storage before preparation was less thanfour hours. Two months cryopreservation using liquid nitrogen (in 40% FBS, 10% DMSO, and RPMI)was conducted. The mean value of CD4 count (cell /mu1) were 522 (G-I), 1410 (G-II), 906 (G-III), 807(G-IV), and 733 (G-V). CD4 count, after 2 month preservation in liquid nitrogen, of the BC sample (G-III) was higher (906 cell /mu1) than PBMC (G-IV) sample (733 cell /mu1).

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How to Cite
NIRURI, Rasmaya et al. CD4 COUNT FROM CRYOPRESERVATION OF BUFFY COAT AND PBMC. International Journal of Biosciences and Biotechnology, [S.l.], v. 2, n. 2, jan. 2016. ISSN 2655-9994. Available at: <https://ojs.unud.ac.id/index.php/jbb/article/view/18157>. Date accessed: 06 july 2020.
Section
Articles

Keywords

CD4; Buffy coat; PBMC; Cryopreservation