Harnessing Serratia marcescens: A Dual Role as Biocontrol Agent Against Fusarium oxysporum f.sp. melongenae and Heteroauxin Producer
Abstract
Pathogens and plant growth factors significantly influence plant growth and development. This study aims to investigate the potential of Serratia marcescens as a biocontrol agent against Fusarium oxysporum f.sp. melongenae and its capability as a heteroauxin producer. Antagonistic tests of S. marcescens against F. oxysporum f.sp. melongenae were conducted using a dual culture method, while the antifungal activity of S. marcescens extract was assessed through the disk diffusion method. Salkowski's colorimetric test was performed both in vitro and in situ to analyze heteroauxin compounds, further identified by GC-MS. Results demonstrated that all four tested S. marcescens strains could inhibit the growth of F. oxysporum f.sp. melongenae, with inhibition rates ranging from 93.76% to 94.02% compared to the control. These strains produced heteroauxin and its derivatives, including 3-methylindole, salicylic acid, and indole-3-acetic acid methyl ester, as confirmed by GC-MS. This study concludes that the examined S. marcescens strains are promising biocontrol agents and heteroauxin producers, highlighting their potential for sustainable agriculture practices.
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References
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Ahmad, M., Khan, M.A., Ahmad, F., Khn, S.M. 1996. Effectiveness of some fungicides on the colony growth of Fusarium oxysporum f.sp. tuberosi and Fusarium solani associated with potato wilt. Pak. J. Phytopathol. 8(2): 159-161.
Arora, P.K., Sharma, A., Bae, H. 2015. Microbial degradation of indole and derivatives. Journal of Chemistry 2015: 1 – 13.
Bunsangiam, S., Nutnaree, T., Savitree, L., Nantana, S. 2021. Large scale production of indole 3 acetic acid and evaluation of the inhibitory efect of indole 3 acetic acid on weed growth. Scientifc Reports 11:13094
Chapman, E.J., Estelle, M. 2009. Mechanism of Auxin- Regulated gene expression in plants. Annu. Rev. Genet. 43: 265 - 285.
Devi, K.A., Piyush, P., Gauri, D.S. 2016. Plant Growth-Promoting endophyte Serratia marcescens AL2-16 enhances the growth of Achyranthes aspera L., a medicinal plant. HAYATI Journal of Biosciences 23: 173-180.
Driesen, E., Wim, V.D.E., Maurice, P., Wouter, S. 2020. Influence of environmental factors light, CO2, temperature, and relative humidity on stomatal opening and development: A Review. Agronomy 10: 2-28.
Gang, S., Sheetal, S., Meenu, S., Martin, B., Jorg, S. 2019. Analysis of Indole-3-acetic Acid (IAA) Production in Klebsiella by LC-MS/MS and the Salkowski Method. Bio-protocol 9(09): 1-9.
Hasuty, A., Choliq, A., Hidayat, I. 2018. Production of Indole Acetic Acid (IAA) by Serratia marcescens subsp. marcescens and Rhodococcus aff. qingshengii. International Journal of Agricultural Technology 14(3): 299-312.
Ismawanti, A., Endang, N., Salman, F., Sumardi. 2022. Effect of Indole Acetic Acid (IAA) by Serratia marcescens strain MBC1 on Soybean (Glycine max L.) Germination. Indonesian Journal of Biotechnology and Biodiversity 6(1):18-25.
Karthick, P., Mohanraju, R., Murthy, K.N., Ramesh, C.H., Mohandass, C., Rajasabapathy, R. 2015. Antimicrobial activity of Serratia sp isolated from the coralline red algae Amphiroa anceps. Indian Journal of Geo-Marine Sciences 44(12): 1857-1866.
Kamnev, A.A., Shchelochkov, A.G., Tarantilis, P.A., Polissiou, M.G., Perfiliev, Y.D. 2001. Spectroscopic investigation of indole-3-acetic acid interaction with iron(III). Journal of Molecular and Structure 563: 565-572.
Kumavath, R.N., Barh, D., Azevedo, V., Kumar, A.P. 2017. Potential pharmacological applications of enzymes associated with bacteral metabolism of aromatic compounds. Journal of Microbiology and Antimicrobials 9 (1): 1 13.
Khalimi, K., Wirya, G.N.A.S. 2010. Pemanfaatan plant growth promoting rhizobacteria untuk biostimulants dan bioprotectans. Ecotrophic 4(2):131-135.
Khalimi, K., Suputra, I.P.W., Wirya, G.A.S., Innosensia, N.L.P.C. 2022. The effectiveness of rhizobacteria as bioprotectants to mitigate Fusarium wilt disease and as biostimulants to improve the growth of chili (Capsicum Annuum). International Journal of Biosciences and Biotechnology 10(1): 26-36.
Lebuhn, M., Heulin, T., Hartmann, A. 1997. Production of auxin and other indolic and phenolic compounds by Paenibacillus polymyxa strains isolated from different proximity to plant roots. FEM Microbiology Ecology 22: 325 – 334.
Makhlouf, B.H., Abdeen, R. 2015. Biological and nanocomposite control of Fusarium wilt of potato caused by Fusarium oxysporum f. sp. tuberosi. Global Journal of Biology, Agriculture and Health Sciences 4(2): 151-163.
Muller, J.L. 2011. Auxin conjugates: their role for plant development and in the evolution of land plants. Journal of Experimental Botany 62 (6): 1757 – 1773.
Nakajima, E., Nakano, H., Yamada, K., Shigemori, H., Hasegawa, K. 2002. Isolation and identification of lateral bud growth inhibitor, indole-3-aldehyde, involved in apical dominance of pea seedlings. Phytochemistry 61: 863 – 865.
Olatunji, D., Geelen, D., Verstraeten, I. 2017. Control of Endogenous Auxin Levels in Plant Root Development.International Journal of Moleculer Sciences 18(2587): 1-29.
Oberhansli, T., Defago, G., Haas, D. 1991. Indole-3-acetic acid (IAA) synthesis in the biocontrol strain CHA0 of Pseudomonas fluorescens: role of tryptophan side chain oxidase. Journal of General Microbiology 137: 2273 - 2279.
Paudel, B., Bhattarai, H.D., Kim, C., Lee, H., Sofronov, R., Ivanova, L., Poryadina, L., Yim, J.H. 2014. Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia. Biological Research 47(10): 1-6.
Purkayastha, G.D., Mangar, P., Saha, A., Saha, D. 2018. Evaluation of the biocontrol efficacy of a Serratia marcescens strain indigenous to tea rhizosphere for the management of root rot disease in tea. PLOS ONE: 1-27.
Queiroz, B.P.V., Melo, I.S. 2006. Antagonism of Serratia marcescens towards Phytophthora parasitica and its effects in promoting the growth of citrus. Brazilian Journal of Microbiology 37:448-450.
Qin, G., Gu, H., Zhao, Y., Ma, Z., Shi, G., Yang, Y., Pichersky, E., Chen, H., Liu, M., Chen, Z., Qua, L.J. 2005. An Indole-3-Acetic Acid Carboxyl Methyltransferase Regulates Arabidopsis Leaf Development. The Plant Cell 17: 2693-2704.
Rechenmann, C.P. 2010. Cellular responses to Auxin: Division versus Expansion. Cold Spring Harbor Perspectives in Biology 2(a001446): 1-15.
Selvakumar, G., Mohan, M., Kundu, S., Gupta, A.D., Joshi, P., Nazim, S., Gupta, H.S. 2007. Cold tolerance and plant growth promotion potential of Serratia marcescens strain SRM (MTCC 8708) isolated from flowers of summer squash (Cucurbita pepo). Letters in Applied Microbiology 46: 171–175.
Vocciante, M., Grifoni, M., Fusini, D., Petruzzelli, G., Franchi, E. 2022. The role of plant growth-promoting rhizobacteria (PGPR) in mitigating plant’s environmental stresses. Appl. Sci. 12(1231): 1-16.
Wang, K., Yan, P.S., Cao, L.X., Ding, Q.L., Shao, C., Zhao, T.F. 2013. Potential of chitinolytic Serratia marcescens strain JPP1 for biological control of Aspergillus parasiticus and aflatoxin. BioMed Research International 2013: 1-8.
Woodward, A.W., Bartel, B. 2005. Auxin: Regulation, action, and interaction. Annals of Botany 95: 707 – 735.
Yang, Y., Xu, R., Ma, C., Vlot, A.C., Klessig, D.F., Pichersky, E. 2008. Inactive methyl indole-3-acetic acid ester can be hydrolyzed and activated by several Esterases belonging to the AtMES esterase family of Arabidopsis. Plant Physiology 147: 1034-1045.
Zhang, C., Yu, Z., Zhang, M., Li, X., Wang, M., Li, L., Li, X., Ding, Z., Tian, H. 2022. Serratia marcescens PLR enhances lateral root formation through supplying PLR-derived auxin and enhancing auxin biosynthesis in Arabidopsis. Journal of Experimental Botany 73(11): 3711-3725.
Ahmad, M., Khan, M.A., Ahmad, F., Khn, S.M. 1996. Effectiveness of some fungicides on the colony growth of Fusarium oxysporum f.sp. tuberosi and Fusarium solani associated with potato wilt. Pak. J. Phytopathol. 8(2): 159-161.
Arora, P.K., Sharma, A., Bae, H. 2015. Microbial degradation of indole and derivatives. Journal of Chemistry 2015: 1 – 13.
Bunsangiam, S., Nutnaree, T., Savitree, L., Nantana, S. 2021. Large scale production of indole 3 acetic acid and evaluation of the inhibitory efect of indole 3 acetic acid on weed growth. Scientifc Reports 11:13094
Chapman, E.J., Estelle, M. 2009. Mechanism of Auxin- Regulated gene expression in plants. Annu. Rev. Genet. 43: 265 - 285.
Devi, K.A., Piyush, P., Gauri, D.S. 2016. Plant Growth-Promoting endophyte Serratia marcescens AL2-16 enhances the growth of Achyranthes aspera L., a medicinal plant. HAYATI Journal of Biosciences 23: 173-180.
Driesen, E., Wim, V.D.E., Maurice, P., Wouter, S. 2020. Influence of environmental factors light, CO2, temperature, and relative humidity on stomatal opening and development: A Review. Agronomy 10: 2-28.
Gang, S., Sheetal, S., Meenu, S., Martin, B., Jorg, S. 2019. Analysis of Indole-3-acetic Acid (IAA) Production in Klebsiella by LC-MS/MS and the Salkowski Method. Bio-protocol 9(09): 1-9.
Hasuty, A., Choliq, A., Hidayat, I. 2018. Production of Indole Acetic Acid (IAA) by Serratia marcescens subsp. marcescens and Rhodococcus aff. qingshengii. International Journal of Agricultural Technology 14(3): 299-312.
Ismawanti, A., Endang, N., Salman, F., Sumardi. 2022. Effect of Indole Acetic Acid (IAA) by Serratia marcescens strain MBC1 on Soybean (Glycine max L.) Germination. Indonesian Journal of Biotechnology and Biodiversity 6(1):18-25.
Karthick, P., Mohanraju, R., Murthy, K.N., Ramesh, C.H., Mohandass, C., Rajasabapathy, R. 2015. Antimicrobial activity of Serratia sp isolated from the coralline red algae Amphiroa anceps. Indian Journal of Geo-Marine Sciences 44(12): 1857-1866.
Kamnev, A.A., Shchelochkov, A.G., Tarantilis, P.A., Polissiou, M.G., Perfiliev, Y.D. 2001. Spectroscopic investigation of indole-3-acetic acid interaction with iron(III). Journal of Molecular and Structure 563: 565-572.
Kumavath, R.N., Barh, D., Azevedo, V., Kumar, A.P. 2017. Potential pharmacological applications of enzymes associated with bacteral metabolism of aromatic compounds. Journal of Microbiology and Antimicrobials 9 (1): 1 13.
Khalimi, K., Wirya, G.N.A.S. 2010. Pemanfaatan plant growth promoting rhizobacteria untuk biostimulants dan bioprotectans. Ecotrophic 4(2):131-135.
Khalimi, K., Suputra, I.P.W., Wirya, G.A.S., Innosensia, N.L.P.C. 2022. The effectiveness of rhizobacteria as bioprotectants to mitigate Fusarium wilt disease and as biostimulants to improve the growth of chili (Capsicum Annuum). International Journal of Biosciences and Biotechnology 10(1): 26-36.
Lebuhn, M., Heulin, T., Hartmann, A. 1997. Production of auxin and other indolic and phenolic compounds by Paenibacillus polymyxa strains isolated from different proximity to plant roots. FEM Microbiology Ecology 22: 325 – 334.
Makhlouf, B.H., Abdeen, R. 2015. Biological and nanocomposite control of Fusarium wilt of potato caused by Fusarium oxysporum f. sp. tuberosi. Global Journal of Biology, Agriculture and Health Sciences 4(2): 151-163.
Muller, J.L. 2011. Auxin conjugates: their role for plant development and in the evolution of land plants. Journal of Experimental Botany 62 (6): 1757 – 1773.
Nakajima, E., Nakano, H., Yamada, K., Shigemori, H., Hasegawa, K. 2002. Isolation and identification of lateral bud growth inhibitor, indole-3-aldehyde, involved in apical dominance of pea seedlings. Phytochemistry 61: 863 – 865.
Olatunji, D., Geelen, D., Verstraeten, I. 2017. Control of Endogenous Auxin Levels in Plant Root Development.International Journal of Moleculer Sciences 18(2587): 1-29.
Oberhansli, T., Defago, G., Haas, D. 1991. Indole-3-acetic acid (IAA) synthesis in the biocontrol strain CHA0 of Pseudomonas fluorescens: role of tryptophan side chain oxidase. Journal of General Microbiology 137: 2273 - 2279.
Paudel, B., Bhattarai, H.D., Kim, C., Lee, H., Sofronov, R., Ivanova, L., Poryadina, L., Yim, J.H. 2014. Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia. Biological Research 47(10): 1-6.
Purkayastha, G.D., Mangar, P., Saha, A., Saha, D. 2018. Evaluation of the biocontrol efficacy of a Serratia marcescens strain indigenous to tea rhizosphere for the management of root rot disease in tea. PLOS ONE: 1-27.
Queiroz, B.P.V., Melo, I.S. 2006. Antagonism of Serratia marcescens towards Phytophthora parasitica and its effects in promoting the growth of citrus. Brazilian Journal of Microbiology 37:448-450.
Qin, G., Gu, H., Zhao, Y., Ma, Z., Shi, G., Yang, Y., Pichersky, E., Chen, H., Liu, M., Chen, Z., Qua, L.J. 2005. An Indole-3-Acetic Acid Carboxyl Methyltransferase Regulates Arabidopsis Leaf Development. The Plant Cell 17: 2693-2704.
Rechenmann, C.P. 2010. Cellular responses to Auxin: Division versus Expansion. Cold Spring Harbor Perspectives in Biology 2(a001446): 1-15.
Selvakumar, G., Mohan, M., Kundu, S., Gupta, A.D., Joshi, P., Nazim, S., Gupta, H.S. 2007. Cold tolerance and plant growth promotion potential of Serratia marcescens strain SRM (MTCC 8708) isolated from flowers of summer squash (Cucurbita pepo). Letters in Applied Microbiology 46: 171–175.
Vocciante, M., Grifoni, M., Fusini, D., Petruzzelli, G., Franchi, E. 2022. The role of plant growth-promoting rhizobacteria (PGPR) in mitigating plant’s environmental stresses. Appl. Sci. 12(1231): 1-16.
Wang, K., Yan, P.S., Cao, L.X., Ding, Q.L., Shao, C., Zhao, T.F. 2013. Potential of chitinolytic Serratia marcescens strain JPP1 for biological control of Aspergillus parasiticus and aflatoxin. BioMed Research International 2013: 1-8.
Woodward, A.W., Bartel, B. 2005. Auxin: Regulation, action, and interaction. Annals of Botany 95: 707 – 735.
Yang, Y., Xu, R., Ma, C., Vlot, A.C., Klessig, D.F., Pichersky, E. 2008. Inactive methyl indole-3-acetic acid ester can be hydrolyzed and activated by several Esterases belonging to the AtMES esterase family of Arabidopsis. Plant Physiology 147: 1034-1045.
Zhang, C., Yu, Z., Zhang, M., Li, X., Wang, M., Li, L., Li, X., Ding, Z., Tian, H. 2022. Serratia marcescens PLR enhances lateral root formation through supplying PLR-derived auxin and enhancing auxin biosynthesis in Arabidopsis. Journal of Experimental Botany 73(11): 3711-3725.
Published
2024-05-01
How to Cite
KHALIMI, Khamdan; PRANATAYANA, Ida Bagus Gde; BATIN, Charlie B.
Harnessing Serratia marcescens: A Dual Role as Biocontrol Agent Against Fusarium oxysporum f.sp. melongenae and Heteroauxin Producer.
International Journal of Biosciences and Biotechnology, [S.l.], v. 11, n. 2, p. 33-44, may 2024.
ISSN 2655-9994.
Available at: <https://ojs.unud.ac.id/index.php/jbb/article/view/114161>. Date accessed: 24 may 2024.
doi: https://doi.org/10.24843/IJBB.2024.v11.i02.p05.
Section
Articles
