Isolasi dan Identifikasi Bakteri Selulolitik pada Sampah Organik di TPA Suwung Denpasar

  • FADEL ALKAHFI Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
  • WAYAN ADIARTAYASA Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
  • I GEDE PUTU WIRAWAN Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana

Abstract

Isolation and Identification of Cellulolytic Bacteria in Organic Waste at TPA Suwung Denpasar


 Organic waste contains a lot of lignocellulosic material which can be degraded by cellulases. The degradation of cellulose requires three types of enzymes produced by microorganisms. Cellulolytic bacteria are one of the microorganisms involved in the decomposition process and produce cellulase enzymes which can degrade organic matter. This study aims to isolate and test the ability of cellulolytic bacteria in the organic waste of TPA Suwung Denpasar by isolating morphological identification, and quantitative cellulase test. The results of this study found twenty-seven (27) bacterial isolates that were able to grow on CMC media and were classified based on colony morphology and obtained 3 different isolate groups. The isolates that had been grouped were coded A, B, and C. The colony of the isolated isolates appeared to be round and wrinkled. Some surfaces are convex, raised, and hilly and are clear, yellows in color, and white. Isolates A and C were gram positive, while isolate B was gram negative. Based on the calculation results of the Cellulolytic Index value, it was found that isolate B had the highest IS and was in the high category.

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Published
2021-04-30
How to Cite
ALKAHFI, FADEL; ADIARTAYASA, WAYAN; WIRAWAN, I GEDE PUTU. Isolasi dan Identifikasi Bakteri Selulolitik pada Sampah Organik di TPA Suwung Denpasar. Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology), [S.l.], p. 153-160, apr. 2021. ISSN 2301-6515. Available at: <https://ojs.unud.ac.id/index.php/jat/article/view/75520>. Date accessed: 04 nov. 2024.
Section
Articles

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