HISTOPATHOLOGICAL DESCRIPTION OF THE LIVER OF WHITE RATS TREATED WITH YEAST
Abstract
This study aimed to determine the histopathological picture of the liver of white rats due to the treatment of yeast at several different doses. A total of 24 white rats were randomly divided into 4 treatment groups, namely P0: control; P1: giving yeast of 100 mg/kg BW; P2: 200 mg/kg BW; and P3: 300 mg/kg BW. The treatment dose was carried out for 21 days. On the 22nd day, all samples got euthanasia and a necropsy procedure was performed. The liver organs were taken to make histological preparations by staining with Hematoxylin Eosin. The variables observed included fatty degeneration and necrosis. The data obtained were analyzed using the Kruskal-Wallis test. The results showed that the administration of yeast at a dose of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW was not significantly different (P>0.05) compared to the control (P0). It can be concluded that threating by yeast up to a dose of 300 mg/kg BW does not cause histopathological changes in the liver of white rats.
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References
Baroroh HN, Warsinah. 2009. Antiinflamatory activity of ethanolic extract of leaves of jarak pagar (Jatropa curcas L.) and neutrophils profile in rats foot induced carrageenan. Proc. International Conference On Medicinal Plants, 22-23.
Dannuri H. 2009. Analisis Enzim Alanin Amino Transferase (ALAT), Aspartat Amino Transferase (ASAT), Urea Darah, dan Histopatologis Hati dan Ginjal Tikus Putih Galur Sprague-Dawley Setelah Pemberian Angklak. Jurnal Teknologi dan Industri Pangan. 20(1): 1-9.
Das SK, Dhanya L, Vasudevan DM. 2008. Biomarkers of alcoholism: an updated review. Scandinav. J. Clin. Lab. Invest. 68: 81-92.
Duhan JAK, Tanwar S. 2013. Bioethanol production from starchy part of tuberous plant (potato) using saccharomyces cerevisiae mtcc-170 Afri. J. Microbiol. 7(46): 5253-5260.
Koivisto H. 2007. Biomarkers for assessing ethanol consumption and the development of alcoholic liver disease: immune responses against ethanol metabolites, cytokine profiles and markers of fibrogenesis. Dissertation. Faculty of Medicine University of Tampere.
Mujdalifah S. 2016. Pengaruh ragi tradisional Indonesia dalam proses fermentasi santan terhadap karakteristik rendemen, kadar air, dan kadar asam lemak bebas virgin coconut oil (VCO). Vortech. 1(1): 10-15.
Oktaviana YA, Dadang S, Endang S. 2015. Pengaruh Ragi Tape terhadap pH, Bakteri Asam Laktat dan Laktosa Yogurt. Fakultas Pertanian, Universitas Bengkulu.
O’Leary VS, Green R, Sullivan BC, Holsinger VH. 2004. Alcohol production by selected yeast strains in lactase hydrolyzed acid whey. J. Biotecnol. Bioengineer. 19(7): 1019-1035.
Onyeka AV, Nwaehujor CO, Ukagwu AL, Nwogwu IC. 2015. Effects of chronic alcohol ingestion on visceral organs in albino mice experimentally challenged with Escherichia coli Strain 0157:H7. Nigeria. Am. J. Pharmacol. Sci. 3(1): 25-32.
Pospos NS. 2002. Bukti gambar, etanol merusak sel hati dan pengaruhnya terhadap konsentrasi ATP intraseluler. Medika. 1(27): 17-20.
Rahmanna AT. 2003. Aflatoksin: senyawa racun pada biji kacang tanah. Bulletin Tani Tanaman Pangan dan Hortikultura.
Saad N. 2001. Aflatoxin Occurence and Health Risk. An undergraduate student Cornell University for The AS625 Class. Animal Science at Cornell University. USA.
Steel RGD, Torrie JH. 1991. Prinsip dan Prosedur Statistika Suatu Pendekatan Biometrik (Terjemahan: Bambang Sumantri). Jakarta. PT. Gramedia.
Steinkraus KH. 1996. Handbook of Indigenous Fermented Foods. Second Edition, Revised And Expanded. New York. Marcel Dekker.
Suaniti NM, Djelantik AAGS, Suastika K, Astawa NM. 2012. Kerusakan hati akibat keracunan alkohol berulang pada tikus wistar. J. Vet. 13(2): 199-204.
Sudira IW, Merdana IM, Winaya IBO, Parnayasa IK. 2019. Perubahan histopatologi ginjal tikus putih diberikan ekstrak sarang semut diinduksi parasetamol dosis toksik. Bul.Vet. Udayana. 11(2): 136-142.
Usmana AS, Rianda S, Novia. 2012. Pengaruh volume enzim dan waktu fermentasi terhadap kadar etanol (Bahan baku tandan kosong kelapa sawit dengan pretreatment alkali). J. Ilm. Teknik Mesin. 18(2): 17-25.
Valeri DC, Samsuri, Berata IK. 2019. Perubahan histopatologi uterus pada tikus putih (rattus norvegicus) akibat pemberian ragi tape. Indon. Med. Vet. 8(3): 338-346.
