HISTOPATHOLOGICAL OF DIABETES MELLITUS WHITE RAT TESTICLE AFTER GIVEN MORINGA LEAF EXTRACT
Abstract
This study aimed to determine changes in testicular histopathological of the rat strain wistar diabetes mellitus experienced. This study used 24 white rats (Rattus norvegicus) strain Wistar which were divided into six groups: administration of Moringa oleifera leaves P1 (extract dose 0 mg/kg bw) , P2 (100 mg/kg bw) , P3 (200 mg/kg bw), P4 (300 mg/kg bw) , P5 (400 mg/kg bw) and P6 ( 500 mg/kg bw) . In the end of administration, the diabetic Wistar rats were sacrificed and their testes were taken to make the histopathological preparations using haematoxylin and eosin staining. The observed variables are fat degeneration and necrosis. The results show that moringa leaves can improve histopathology testis structure of diabetes mellitus white rats. Extract of Moringa leaves (Moringa oleifera) dose of 400 mg/kg body weight has the best effect repair damage to the histopathological testis.
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References
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Dewi YF, Anthara MS, Dharmayudha AAGO. 2014. Efektifitas Ekstrak Daun Sirih Merah (Piper crocatum) Terhadap Penurunan Kadar Glukosa Darah Tikus Putih Jantan (Rattus novergicus) Yang Di Induksi Aloksan. Bul. Vet. Udayana. 6(1): 73-79.
Al-Malki AL, El Rabey HA. 2015. The antidiabetic effect of low doses of Moringa oleifera lam: seeds on streptozotocin induced diabetes and diabetic nephropathy in male rats. Biomed. Res. Int. 2015: 1-13.
Berata IK, Winaya IBO, Adi AAAM, Adnyana IBW. 2011. Patologi Veteriner Umum. Denpasar. Swasta Nulus.
Dharmayudha AAGO, Antara MS. 2013. Identifikasi Golongan Senyawa Kimia dan Pengaruh Ekstrak Etanol Buah Naga Daging Putih (Hylocereus undatus) Terhadap Penurunan Kadar Glukosa Darah Serta Bobot Badan Tikus Putih Jantan (Rattus norvegicus) yang diinduksi Aloksan. Bul. Vet. Udayana. 5(1): 31-40.
Dillard CJ, German JB. 2000. Review Phytochemicals: nutraceuticals and human health. J. Sci. Food Agric. 80: 1744-1746.
Fall T, Hamlin HH, Hedhammar A, Kampe O, Egenvall A. 2007. Diabetes Mellitus in a Population of 180.000 Insured Dogs: Incidence, Survival, and Breed Distribution. J. Vet. Intern. Med. 21: 1209-1216.
Halliwell B, Gutteridge JMC. 2007. Free Radical in Biology and Medicine. 4th Ed, Oxford University Press, New York. Pp. 19-633.
Kasolo JN, Bimenya GS, Lonzy O, Ochieng J, Jasper W, Ogwal O. 2010. Phytochemicals and uses of moringa oleifera leaves in Ugandan rural communities. J. Med. Plants Res. 4(9): 753-757.
Lilibeth A, Cajuday. 2010. Effects of Moringa oleifera Lam. (Moringaceae) on The Reproduction of Male Mice (Mus musculus L). J. Med. Plants Res. 4(12): 1115-1121.
Sampurna IP, Nindhia TS. 2008. Analisis Data Dengan SPSS: Dalam Rancangan Percobaan. Udayana University Press. Denpasar.
Seethalakshmi L, Menon M, Diamond D. The effect of streptozotocin-induced diabetes on the neuroendocrine-male reproductive tract axis of the adult rat. J. Urol. 138: 190-194.
Siddhuraju P, Becker K .2003. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J. Agric. Food Chem. 51: 2144-2150.
Suastika, P. 2011. Efek Pemberian Buah Merah (Pandanus concideus) terhadap perubahan Histopatologik Ginjal dan Hati mencit Pasca Pemberian Paracetamol. Bul. Vet. Udayana. 3(1): 31-44.
Suyono S. 2006. Buku Ajar Ilmu Penyakit Dalam Jilid III, 4th Ed. Diabetes Mellitus di Indonesia; Suyodo, Setiyohadi, Alwi I, Simadibrata, Setiati (eds), Balai Penerbit Fakultas Kedokteran, Universitas Indonesia, Jakarta. Pp. 1852-1856.
Unger RH, Foster DW. 1992. Diabetes Mellitus, In Wilson, J.D. dan Foster, D.W., Endocrinologi, 1255-1317, W.B Sunders Compani, A Division of Harcourt Brace dan Company, London.
Yassa HD, Tohamy AF. 2014. Extract of Moringa oleifera leaves ameliorates streptozotocin-induced Diabetes mellitus in adult rats. Acta Histochemica. 116(5): 844-854.
Dewi YF, Anthara MS, Dharmayudha AAGO. 2014. Efektifitas Ekstrak Daun Sirih Merah (Piper crocatum) Terhadap Penurunan Kadar Glukosa Darah Tikus Putih Jantan (Rattus novergicus) Yang Di Induksi Aloksan. Bul. Vet. Udayana. 6(1): 73-79.
Published
2018-08-27
How to Cite
JANNAH, Raodatul; SETIASIH, Ni Luh Eka; SUASTIKA, Putu.
HISTOPATHOLOGICAL OF DIABETES MELLITUS WHITE RAT TESTICLE AFTER GIVEN MORINGA LEAF EXTRACT.
Buletin Veteriner Udayana, [S.l.], p. 176-182, aug. 2018.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/32659>. Date accessed: 18 apr. 2024.
doi: https://doi.org/10.24843/bulvet.2018.v10.i02.p11.
Section
Articles
