• Yoga Pratama Nuradi
  • I Nyoman Suartha
  • Ida Bagus Komang Ardana


Glycyrrhizic acid, which is the active ingredient of Viusid© Pet, is commonly used to enhance the immune response. This study was conducted to determine the effect of Viusid© Pet on macrophage activity and capacity. Thirty-six BALB/c mice were divided into 3 groups. The first group as control was given aquades, the second group was given a dose of Viusid© Pet 0.1 ml, and the third group was given a dose of Viusid© Pet 0.2 ml. All groups were given treatment from the first day until the seventh day. On the eighth day, each mouse was injected intraperitoneally by Staphylococcus aureus bacteria. The activity and capacity of macrophage cells were calculated from the peritoneal fluid smear by calculating the percentage of phagocytes performing phagocytosis in 100 macrophage cells. The phagocytic capacity was determined by the amount of Staphylococcus aureus bacteria that is phagocytosed by 50 active phagocyte cells. The activity of phagocytosis increases with increasing doses of Viusid © Pet. It was concluded that the highest macrophage activity and capacity was achieved in the treatment group with the dosage of Viusid © Pet 0.2 ml (825.33 ± 291,73). While the activity and the lowest phagocytic capacity of the negative control group (578.08 ± 186.94) was followed by the dose group of 0.1 ml (654.83 ± 266.09).


Download data is not yet available.

Author Biographies

Yoga Pratama Nuradi

Veterinarian practitioners in West Java

I Nyoman Suartha

Laboratory of Internal veterinarian medicine, Faculty of Veterinary Medicine

Ida Bagus Komang Ardana

Laboratory of Veterinary Clinical Pathology, Udayana University


Abbas AK, Lichtman AH. 2000. Cellular and Molecular Immunology. 4th ed. WB Saunders Company Saunders, Philadelphia. Pp: 19-347.
Abbas AK, Litchman AH. 2010. Basic Immunology. Update 3rd Ed. Philadelphia: WB Saunders Company.
Beer DJ, Charles AD., Lanny JR, Ross ER, 1982. Human Monocyte-derived Soluble Product(s) Has an Accessory Function in the Generation of Histamine- and Concanavalin A-induced Suppressor T cells. J Clin Invest 70: 393-400.
Besung, INK. 2009. Pegagan (Centella Asiatica) sebagai Alternatif Pencegahan Penyakit Infeksi pada Ternak. Bulletin Veteriner Udayana 1(2): 61-67.
Besung, INK. 2011. Pengaruh Ekstrak Pegagan (Centella Asiatica) dalam Meningkatkan Kapasitas Fagosit Makrofag Peritoneum Mencit terhadap Salmonella Typhi. Bulletin Veteriner Udayana 3(2): 71-78.
Bratawidjaja, K. 2002. Imunologi Dasar. Ed. IV. Fakultas Kedokteran. Universitas Indonesia. Jakarta.
Brocker, C, Thompson D, Matsumoto A, Nebert, DW, Vasiliou, V. 2010. Evolutionary divergence and functions of the human interleukin (IL) gene family. Hum Genomics 5(1): 30-55.
Dai JH, Yasumasa I, Takaomi I, Hiroshi T, Hirotake K, Yoichiro I, Hiromi F. M. I. 2001. Glycyrrhizin enhances interleukin-12 production in peritoneal macrophages. Immunology 103: 235-243.
Gomez, EV, Rodriguez MA, Gra OB, Arus SE, Llanio NR, Caldazzila BL, Yassels GA, Del Rosario AVM. 2009. Clinical trial: a nutritional supplement Viusid© , in combination with diet and exercise, in patients with nonalcoholic fatty liver disease. Alimentary Pharmacology & Therapeutics 30:999-1009.
Gomez, EV, Yoan SR, Ana TG, Luiz CB, Enrique AS, Yadina MP, Ali YG, Maria dRAV. 2011. Viusid© , a nutritional supplement, increases survival and reduces disease progression in HCV-related decompensated cirrhosis: a randomized and controlled trial. BMJ Open 1:1-11.
Gomez, JD, Ramon DS, Alfredo AD and Hana Z. 2012. Clinical Study Effectiveness of Glycyrrhizinic Acid (Glizigen) and an Immunostimulant (Viusid© ) to Treat Anogenital Warts. ISRN Dermatology 2012: 1-6.
Greenberg S, Peter C, Samuel CS. 1993. Tyrosine Phosphorylation Is Required for Fc Receptor-mediated Phagocytosis in Mouse Macrophages. J Exp Med 177: 529-534.
Hoffman, T, Young LL, Elaine FL, Anil KT, Ezio B, Joseph P. 1992. Differential turnover of enzymes involved in human monocyte eicosanoid metabolism: Selective inhibition of cyclooxygenase product formation by cycloheximide in the absence of effects on 5-lipoxygenase or phospholipase A2. Biochemical Pharmacology 44(5): 955–963.
Karlsson F, Carl AKB, Nina N, Ann-Christin MH. 2003. The Mechanism of Bacterial Infection by Filamentous Phages Involves Molecular Interactions between TolA and Phage Protein 3 Domains. J Bacteriol 185: 2628–2634.
Kusmardi, Shirly K, Enif ET. 2007. Efek Imunomodulator Ekstrak Daun Ketepeng Cina (Cassia alata L.) Terhadap Aktivitas Dan Kapasitas Fagositosis Makrofag. Makara 11: 50-53.
Lee, CH, Sang WP, Yeong SK, Sam SK, Jeong AK, Seung HL, Sun ML. 2007. Protective Mechanism of Glycyrrhizin on Acute Liver Injury Induced by Carbon Tetrachloride in Mice. Biol Pharm Bull 30: 1898-1904.
Merdana, IM. 2010. Uji Bioaktivitas Antibakteri Tanaman Obat Tradisional. Bulletin Veteriner Udayana 2(1): 51-56.
Queiroz-Junior, CM, Marcelo JBS, Joice DC, Mila FMM, Thiago PG, Gustavo PG, Fernando QC, Mauro MT, Tarcılia AS. 2010. A Controversial Role for IL-12 in Immune Response and Bone Resorption at Apical Periodontal Sites. Clin and DevelopImmunol 2010: 1-8.
Tjahjadi, S, Tri HA, Muchtan S, Ridad A, Din S. 2008. Asam L askorbat meningkatkan aktivitas antimalaria artemisinin bergantung konsetrasi. Majalah Kedokteran Bandung 40: 176-180.
How to Cite
NURADI, Yoga Pratama; SUARTHA, I Nyoman; ARDANA, Ida Bagus Komang. THE EFFECT OF VIUSID© PET TO ACTIVITY AND CAPASITY OF MACROPHAGES IN MICE. Buletin Veteriner Udayana, [S.l.], p. 171-177, aug. 2017. ISSN 2477-2712. Available at: <>. Date accessed: 22 june 2021.

Most read articles by the same author(s)

1 2 3 > >>