PHYSICHOCHEMICAL CHACTERIZATION OF BACTERIOCIN PRODUCING ENTEROCOCCUS DURANS ISOLATED FROM COLON’S BALI CATTLE
Abstract
Lactic acid bacteria can excrete antimicrobial compounds like bacteriocins. The study aimed to find out the characteristic of physic-chemical of bacteriocin producing Enterococcus durans isolate 18A isolated from colon’s bali cattle. The study initiated by Gram staining and catalase test, followed by isolation and purification of bacteriocin. The result of the research showed that bacteriocins of Enterococcus durans isolate 18A as a protein with it’s concentrationis 0,272?g/ml and it does not contain carbohydrate. On the other hand, the bacteriocins was not showed a band while tested on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The result showed, the bacteriocin producing Enterococcus durans isolate 18A has antimicrobial activity to Bacillus cereus as 23,88%.
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References
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Tagg JR, Dajani AS, Wannamaker LW. 1979. Bacteriocins of Gram-Positive Bacteria. Bacteriological Reviews. 40 (3) : 722-756
Van den Berghe E, Skourtas G, Tsakalidou E, De Vuyst L. 2006. Streptococcus macedonicus ACADC 198 produces the lantibiotic, macedocin, at temperature and pH conditions that prevail during cheese manufacture. Int J Food Microbiol. 107 : 138–147.
Walker JM. 1984. Gradient SDS polyacrylamine gel electrophoresis, Methods in Molecular Biology, Vol.1, The Humana Press Inc., Clifton, NJ. Hlm 57–61.
Widyastuti Y, Sofarianawati E. 1999. Karakter Bakteri Asam Laktat Enterococcus sp . yang Diisolasi dari Saluran Pencernaan Ternak. Jurnal Mikrobiologi Indonesia. 4 (2) : 50-53.
Brooks G, Butel JS, Morse SA. 2005. Mikrobiologi kedokteran. Alih Bahasa. Mudihardi E, Kuntaman,WasitoEB et al. Jakarta: Salemba Medika: 317-27.
De Vuyst L. 2007. Bakteriosins from Lactic Acid Bacteria: Production, Purification, and Food Apllication. J of Microbial and Biotech.13 (4): 194-199.
Garneau S, Martin NI, Vederas JC. 2002. Two – Peptide Bacteriocins Produced by Lactic Acid Bacteria. Biochimie. 84 : 557-592
Gonzallez B, Glaasker E, Kinji ERS, Driessen AJM, Suares JE, Konings WN. 1996. Bacterial Mode of Action of Plantaricin C. App Environ Microbiol. 62 : 2701-2709
Hafsan. 2014. Bakteriosin Asal Bakteri Asam Laktat Sebagai Biopreservatif Pangan. J Teknosis. 8 (2) : 175-184
Harborne JB. 1984. Phytochemical Methods. Ed ke-2. New York: Chapman and Hall.
Jay M, James. 1992. Modern Food Microbiology. Fourth Edition. New York: Michigan publising.
Klaenhammer TR. 1998. Bacteriocins of Lactic Acid Bacteria. Departments of Food Science and Microbiology, North Carolina State University, Raleigh, NC 27695-7624, USA. Biochimie. 70 : 337-349.
Kusmiati, Malik A. 2002. Aktivitas Bakteriosin Bakteri Leuconostoc mesenteroides Pbac 1 pada Berbagai Media. Makara Kesehatan. 6 (1):1-7
Karthikeyan V, Santhosh SW. 2009. Study of Bacteriocin as a Food Preservative and the L. acidophilus Strain as Probiotic. Pakistan J of Nutrition 8 (4) : 335-340
Lay BW. 1994. Analisa Mikroba di Laboratorium. Jakarta: Raja Grafi ndo Persada. Hlm 168.
Leroy LDVF. 2007. Bacteriocins from Lactic Acid Bacteria: Production, Purification, and Food Applications. J Mol Microbiol Biotechnol. 13 : 194–199
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193 : 265–275.
Ness IF, Diep DB, Holo H. 2007. Bacteriocin Diversity in Streptococcus and Entericoccus J of Bact. 189 (4) : 1189-1198.
Nigam A, Ayyagari A. 2007. Lab Manual in Biochemistry, Immunology, and Biotechnology. Tata MCGraw-Hill Publishing Company Limited : New Delhi.
Norman SJ, Edward SA, Boris EV, Yuri KN, Larisa VI, Vladimir PV. 2005. Paenibacillus polymyxa Purified Bacteriocin To Control Campylobacter jejuni in Chickens. J of Food Protection. 2005; 68 (7) : 1450-1453.
Pingitore EV, Salvucci E, Sesma F, Nader-Macias ME. 2007. Different strategies for purification of antimicrobial peptides from lactic acid bacteria (LAB). Dalam: Vilas AM (Ed.) Communicating Current Research and Educational Topics and Trend in Apl. Microb: 557-568.
Suarsana IN. 2011. Karakterisasi Fisikokimia Bakteriosin Yang Diekstrak Dari Yoghurt. Buletin Veteriner. 3 (1) : 1-8.
Suardana IW, Suada IK, Sukada IM, Suarsana IN. 2009. Isolasi dan Identifikasi Bakteri Asam Laktat SR9 Asal Cairan Rumen Sapi Bali Sebagai Kandidat Unggul Probiotik. J. Ilmiah Kedokteran. 8 (2) : 100.
Suardana IW, Suarsana IN, Sujaya IN, Wiryawan KG. 2007. Isolasi dan Identifikasi Bakteri Asam Laktat dari Cairan Rumen Sapi Bali Sebagai Kandidat Biopreservatif J Veteriner. 8 (4) : 155-159.
Suardana IW. 2013a. Potensi Isolat Lactobacillus brevis Asal Cairan Rumen Sapi Bali sebagai Sumber Senyawa Antimikroba dalam Prosiding Seminar Nasional Sapi Bali. Bali 24 September 2013:87-97.
Suardana IW. 2013b. Kajian Pola Pertumbuhan dan Aktivitas Antimikroba Isolat Lactococcus lactis spp lactis 1 Asal Cairan Rumen Sapi Bali dalam “Buku Karya Unud untuk Anak Bangsa”: 50-57.
Suardana IW. 2014. Aplikasi Bakteriosin Asal Yoghurt sebagai Biopreservatif Daging Ayam pada Penyimpanan Suhu Dingin. Prosiding Seminar Nasional Sains dan Teknologi 2014. Denpasar, 18-19 September 2014.: 362-372
Suardana IW, Cahyani AP, Pinatih KJP. 2016. Probiotic Potency and Molecular Identification of Lactic Acid Bacteria Isolated from Bali Cattle’s Colon, Indonesia. Glo.Adv.J.Med.Med.Sci. 5(5): 143-149
Subagyo WC, Suwiti NK, Suarsana IN. 2015. Karakteristik Protein Daging Sapi Bali Dan Wagyu Setelah Direbus. Buletin Veteriner. 7 (1) : 1-25.
Sudirman I, Mathiau F, Michael M, Lefebvre G. 1993. Detection and Properties of Curvaticin 13, a Bacteriocin Like Substance Produced by Lactobacillus curvatus SB 13. Current Microbiol 27 : 35-40.
Tagg JR, Dajani AS, Wannamaker LW. 1979. Bacteriocins of Gram-Positive Bacteria. Bacteriological Reviews. 40 (3) : 722-756
Van den Berghe E, Skourtas G, Tsakalidou E, De Vuyst L. 2006. Streptococcus macedonicus ACADC 198 produces the lantibiotic, macedocin, at temperature and pH conditions that prevail during cheese manufacture. Int J Food Microbiol. 107 : 138–147.
Walker JM. 1984. Gradient SDS polyacrylamine gel electrophoresis, Methods in Molecular Biology, Vol.1, The Humana Press Inc., Clifton, NJ. Hlm 57–61.
Widyastuti Y, Sofarianawati E. 1999. Karakter Bakteri Asam Laktat Enterococcus sp . yang Diisolasi dari Saluran Pencernaan Ternak. Jurnal Mikrobiologi Indonesia. 4 (2) : 50-53.
Published
2017-08-30
How to Cite
SUARDANA, I Wayan; SEPTIARA, Hana Kristal Alamanda; SUARSANA, I Nyoman.
PHYSICHOCHEMICAL CHACTERIZATION OF BACTERIOCIN PRODUCING ENTEROCOCCUS DURANS ISOLATED FROM COLON’S BALI CATTLE.
Buletin Veteriner Udayana, [S.l.], p. 209-215, aug. 2017.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/31407>. Date accessed: 22 nov. 2024.
Section
Articles
