PENGARUH PEMBERIAN KITOSAN TERHADAP KADAR MINERAL DAN KOLESTEROL SERUM KELINCI

Abstract

ABSTRAK Kitosan adalah polimer polisakarida merupakan suplemen diet yang telah luas digunakan di bidang kedokteran untuk penurun kolesterol dan mengurangi berat badan. Penelitian ini bertujuan untuk mengetahui pengaruh konsumsi kitosan terhadap kadar kolesterol, trigliserida, kadar glukosa darah serta terhadap kadar mineral kalsium dan fosfat dalam serum. Sebanyak 12 ekor kelinci jantan ras New Zealand white berumur 5 bulan dengan bobot badan antara 1,5 – 1,6 kg telah digunakan dalam penelitian ini. Hewan percobaan dibagi menjadi empat kelompok perlakuan, yaitu kelompok I (kontrol negatif), kelompok II-IV, yaitu kelompok perlakuan yang diberi kitosan masing-masing 1, 2, dan 4%. Kadar total kolesterol dan trigliserida serum di analisis menggunakan metode kalorometri enzimatis, kadar glukosa darah dianalisis dengan metode biosensor glukose oksidase, kadar kalsium metode spektrophotometri serapan atom (AAS), dan fosfat dengan metode spektrophotometri. Hasil penelitian menunjukkan bahwa pemberian kitosan 2% dan 4% dapat menurunkan kadar total kolesterol, trigliserida, kalsium, dan fosfat serum serta berbeda nyata (P<0,05) dibanding dengan kontrol negatif, dan tidak berpengaruh terhadap kadar glukosa darah. THE EFFECT OF ADMINISTRATION CHITOSAN ON MINERAL AND CHOLESTEROL CONCENTRATION SERUM IN RABBITS ABSTRACT Chitosan is a polysaccharide polymer is a dietary supplement that has been widely used in medicine for lowering cholesterol and reducing weight. This study aims to determine the effect of chitosan intake on cholesterol, triglycerides, blood glucose levels as well as the minerals calcium and phosphate levels in serum. A total of 12 male rabbits of New Zealand white 5 months old with body weight between 1.5-1.6 kg were used in this study. The animals were randomly divided into four treatment groups: group I (negative control), group II-IV, namely chitosan treatment group were given respectively 1%, 2%, and 4%. Levels of total serum cholesterol and triglycerides in analysis kalorometri enzymatic method, blood glucose analysis by glucose oxidase biosensor method, calcium by atomic absorption spectrophotometri method (AAS), and phosphate spectrophotometri method. The results showed that administration of chitosan 2% and 4% can reduce total cholesterol, triglycerides, calcium, and phosphate and serum significantly different (P<0.05) compared with negative control, and no effect on blood glucose levels.