PHYSICHOCHEMICAL CHACTERIZATION OF BACTERIOCIN PRODUCING ENTEROCOCCUS DURANS ISOLATED FROM COLON’S BALI CATTLE

  • I Wayan Suardana
  • Hana Kristal Alamanda Septiara
  • I Nyoman Suarsana

Abstract

Lactic acid bacteria can excrete antimicrobial compounds like bacteriocins. The study  aimed  to  find  out  the  characteristic  of  physic-chemical  of  bacteriocin  producing Enterococcus durans          isolate 18A isolated from  colon’s bali cattle. The study initiated by Gram staining and catalase test, followed by isolation and purification  of bacteriocin.       The result of the research showed  that bacteriocins of    Enterococcus durans    isolate 18A as a protein with it’s concentrationis  0,272?g/ml  and  it  does  not  contain  carbohydrate.  On  the  other  hand,  the bacteriocins was not showed a band while tested on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The result showed, the bacteriocin producing            Enterococcus durans isolate 18A has antimicrobial activity to Bacillus cereus  as 23,88%.

Author Biographies

I Wayan Suardana

Laboratory of Veterinary Public Health Faculty of Veterinary Medicine Udayana University

Hana Kristal Alamanda Septiara

Veterinarian practitioners in Denpasar

I Nyoman Suarsana

Laboratory of Veterinary Biochemistry, Udayana University 

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Published
2017-08-30
How to Cite
SUARDANA, I Wayan; SEPTIARA, Hana Kristal Alamanda; SUARSANA, I Nyoman. PHYSICHOCHEMICAL CHACTERIZATION OF BACTERIOCIN PRODUCING ENTEROCOCCUS DURANS ISOLATED FROM COLON’S BALI CATTLE. Buletin Veteriner Udayana, [S.l.], p. 209-215, aug. 2017. ISSN 2477-2712. Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/31407>. Date accessed: 11 nov. 2019.
Section
Articles