• I Gusti Made Krisna Erawan
  • Ida Tjahajati
  • Wisnu Nurcahyo
  • Widya Asmara


Dirofilaria immitis (D. immitis) as a causative agent of heartworm disease is not only caused problems in animals but also zoonoses.  For diagnosis of dirofilariasis (disease caused by D. immitis) serologically is needed anti-D. immitis antibodies.  The objective of this study was to produce antibodies against excretory-secretory antigens produced by male and female worms for developing the diagnosis of dirofilariasis based on the antigens detection.  Base on this study can be concluded that male excretory-secretory antigens (MES), female excretory-secretory antigens (FES), and MES+FES can stimulate BALB/c mouse to produce polyclonal antibodies in the same pattern. Antibodies have been produced at day 21 and the peak titter was at day 35 after first immunization.


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Author Biographies

I Gusti Made Krisna Erawan

Laboratory of Internal Veterinary Medicine, Faculty of Veterinary Medicine

Ida Tjahajati

Department of Internal Veterinary Medicine, Faculty of Veterinary Medicine Gadjah Mada University

Wisnu Nurcahyo

Department of Veterinary Parasitology, Gadjah Mada University

Widya Asmara

Deparment of Veterinary Bacteriology, Gadjah Mada University


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How to Cite
ERAWAN, I Gusti Made Krisna et al. THE PRODUCTION OF ANTI-Dirofilaria immitis ANTIBODIES FOR THE DIAGNOSIS DEVELOPMENT OF DIROFILARIASIS IN DOGS. Buletin Veteriner Udayana, [S.l.], aug. 2016. ISSN 2477-2712. Available at: <>. Date accessed: 01 june 2023.


Dirofilaria immitis; antibodi; antigen ekskretori-sekretori; antibodies; excretory-secretory antigens