Produksi Antibodi Anti-Dirofilaria immitis Untuk Pengembangan Diagnosis Dirofilariasis Pada Anjing
Abstrak
Dirofilaria immitis (D. immitis) sebagai agen penyebab penyakit cacing jantung tidak hanya menimbulkan masalah pada hewan tetapi juga bersifat zoonosis. Untuk mendiagnosis dirofilariasis (penyakit yang disebabkan oleh D. immitis) secara serologis dibutuhkan antibodi anti-D. immitis. Penelitian ini bertujuan untuk memproduksi antibodi terhadap antigen ekskretori-sekretori cacing jantan dan cacing betina untuk pengembangan diagnosis berbasis deteksi antigen. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa antigen ekskretori-sekretori jantan (male excretory-secretory antigens/MES), antigen ekskretori-sekretori betina (female excretory-secretory antigens FES) dan antigen ekskretori-sekretori jantan dicampur dengan betina (MES+FES) D. immitis dapat merangsang pembentukan antibodi poliklonal pada mencit BALB/c dengan pola produksi yang sama. Antibodi telah terbentuk pada hari ke-21 dan titernya mencapai puncak pada hari ke-35 setelah imunisasi.
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