Identifikasi Spesies Stafilokokus Pada Ikan Kerapu Di Kabupaten Karangasem Dengan Analisis Sekuen 16S rRNA (SPECIES IDENTIFICATION OF STAPHYLOCOCCUS ON GROUPER IN KARANGASEM REGENCY BASED ON 16S RIBOSOMAL RNA SEQUENCE ANALYSIS)

  • I Nengah Kerta Besung
  • Ketut Wella Mellisandy
  • I Gusti Ngurah Kade Mahardika

Abstract

This study aims to identify the species Staphylococcus grouper in Karangasem Regency.Faeces samples were collected and grown on media Blood order and identified by Gram staining.Suspected staphylococcal bacterial culture, included in the 10% Chelex media. The 16S rRNAgene fragments amplified from DNA (deoxyribonucleic acid) by Polymerase Chain Reaction(PCR) with specific primers that is Tsta G422 and Tstag 765. PCR (Polymerase Chain Reaction)products were sequenced in Barkley Sequencing Facility in the USA The result of sequencing wasedit and sorted using MEGA 5.0. Final sequence from each isolate was aligned using database inĀ GenBank by fitur of BLAST. Several of standard sequences were downloaded to construct thefilogenetik tree. Species which can be confimed was Staphylococcus warneriwith homology valueof 100% with the data in GenBank.

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Author Biographies

I Nengah Kerta Besung
Laboratorium Mikrobiologi Veteriner Universitas Udayana
Ketut Wella Mellisandy
Mahasiswa Fakultas Kedokteran Hewan Universitas Udayana
I Gusti Ngurah Kade Mahardika
Laboratorium Biomedik Veteriner Universitas Udayana
Published
2015-02-13
How to Cite
BESUNG, I Nengah Kerta; MELLISANDY, Ketut Wella; MAHARDIKA, I Gusti Ngurah Kade. Identifikasi Spesies Stafilokokus Pada Ikan Kerapu Di Kabupaten Karangasem Dengan Analisis Sekuen 16S rRNA (SPECIES IDENTIFICATION OF STAPHYLOCOCCUS ON GROUPER IN KARANGASEM REGENCY BASED ON 16S RIBOSOMAL RNA SEQUENCE ANALYSIS). Buletin Veteriner Udayana, [S.l.], feb. 2015. ISSN 2477-2712. Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/19639>. Date accessed: 28 feb. 2021.
Section
Articles

Keywords

grauper; Staphyloccocus; PCR; 16S rRNA

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