ADDITION OF VITAMIN E IN SEMENLIFE® DILUENT ON QUALITY AND SHELF LIFE OF SPERMATOZOA OF LANDRACE PIGS
Abstract
Artificial insemination in pigs in Indonesia still uses liquid semen. Therefore, specific media are needed to maintain the quality of pig spermatozoa. This study aims to determine the effect of adding vitamin E (a-tocopherol) to Semenlife® (INTTEC) diluent on the quality and shelf life of spermatozoa of landrace pigs. The sample used was fresh semen which was collected using a mass technique taken from the UPTD. Regional Artificial Insemination Center, Livestock Breeding and Forage/Livestock Institute of Bali Province. A total of 24 samples consisting of 12 samples in the SemenLife® (INTTEC) diluent and 12 samples in the SemenLife® (INTTEC) diluent + vitamin E 400 µg/ml were stored at 15-18oC. Observation of spermatozoa quality (viability and motility) and storage time was carried out for 120 hours with observations of spermatozoa quality under a microscope every 24 hours, then DNA fragmentation was tested using the Pocrine 8-Hydroxy-desoxyguanosine ELISA Kit. The graph results of Duncan's test showed that the addition of 400 µg/ml vitamin E had a significant effect on the quality of spermatozoa, especially in terms of motility which was maintained at 48 hours and the lowest DNA fragmentation occurred at 72 hours. Furthermore, the addition of vitamin E was not able to extend the shelf life of landrace pig spermatozoa in diluent. Where, there was a decrease in motility and an increase in DNA fragmentation, respectively at 72 and 96 hours, in viability the decrease occurred periodically from the 0 hour to 120 hours. From the results of the quality and shelf-life tests obtained, spermatozoa can then be tested by conducting a fertility test to find out whether the results obtained can increase litter size.
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References
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Aritonang. 1993. Beternak babi. Penerbit Mutiara, Jakarta.
Awda, Basim J, Mackenzie-Bel Ml, Marry M, Buhr. 2009. Reactive Oxygen Species and Boar Sperm Function. Biol. Reprod. 8: 553-561.
Bebas W, Agustina KK. 2022. Short Communication: The use of lactose-astaxanthin to maintain the quality of Green junglefowl frozen semen. Biodiversitas. 23(11): 5759-5764.
Bebas W, Gorda IW. 2020. The best level of glycerol and dimethylsulfoxide cryoprotectant in the astaxanthin phosphate duck egg yolk extender on the quality of boar frozen semen. J. Vet. 21(1): 115-123.
Bebas W, Geovany LB, Made KB. 2016. Penambahan Vitamin E Pada Pengencer BTS® Terhadap Daya Hidup Dan Motilitas Spermatozoa Babi Landrace Pada Penyimpanan 15° C. Bul. Vet. Udayana. 8(1): 1-7.
Breininger E, Beorlegui NB, OFlaherty CM. 2004. Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen. Theriogenol. 63: 2126- 2135.
Brotosudarmo THP, Limantara L, Setiyono E, Heriyanto. 2020. Structures of astaxanthin and their consequences for therapeutic application. Int. J. Food Sci. 2020: 2156582.
Budaarsa K, Puger AW, Suasta IM. 2016. Ekplorasi komposisi pakan tradisional babi bali. Maj. Ilmiah Pet. 19(1): 6-11.
Chatterjee S, De Lamirande, Gagnon E. 2001. Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: protection by oxidized glutathione. Mol. Reprod. Dev. 60: 498-506.
Dethan AA, Kustono, Hartadi H. 2010. Kualitas dan Kuantitas Sperma Kambing Bligon Jantan yang Diberi Pakan Rumput Gajah dengan Suplementasi Tepung Darah. Bul. Pet. 34(3): 145-153.
Dose J, Matsugo S, Yokokawa H, Koshida Y, Okazaki S, Seidel U, Eggersdorfer M, Rimbach G, Esatbeyoglu T. 2016. Free radical scavenging and cellular antioxidant properties of astaxanthin. Int. J. Mol. Sci. 17(1): 103.
Feka WV, Dethan AA, Beyleto VY. 2016. Pengaruh Lama Penyimpanan terhadap Viabilitas dan pH Semen Babi Landrace yang Diencerkan Menggunakan Bahan Pengencer Sitrat Kuning Telur. JAS. 1(3): 34-35.
Gadea J. 2003. Semen extenders used in the artificial insemination of swine. Spanish J of Agri Research. 1(2): 17-27.
Garner DL, Hafez ESE. 2000. Spermatozoa and seminal plasma. In: Hafez B. Hafez ESE, editor. Reproduction in Farm Animals. 7th Ed. Philadelphia (US): Lippincott Williams and Wilkins. Pp. 96-109.
Guo HT, Wang JR, Sun LZ, Jin XH, Shi XY, Lin JY, Yue SL, Zhou JB. 2021. Effects of astaxanthin on plasma membrane function and fertility of boar sperm during cryopreservation. Theriogenol. 164: 58- 64.
Hartono M. 2008. Optimalisasi penambahan vitamin E dalam pengencer sitrat kuning telur untuk mempertahankan kualitas semen kambing boer. J. Indon. Trop. Anim. Agric. 33: 1-9.
Hofer T. 2001. Method Development of Analysis of 8-oxodG as a Biomarker for Oxidative Stress. Departemen Biosains: Institut Karolinska.
Hsieh YY, Chang CC, Lin CS. 2006. Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility. Int. J. Biol. Sci. 2: 23-29.
Johnson LA, Weitze KF, Fiser P, Maxwell WMC. 2000. Storage of boar semen. J. Anim. Sci. 62: 143-172.
Juan CA, Pérez de la Lastra JM, Plou FJ and Pérez-Lebeña E, 2021. The chemistry of reactive oxygen species (ROS) Revisited: outlining their role in biological macromolecules (DNA, Lipids and Proteins) and induced pathologies. Int. J. Mol. Sci. 22(9): 4642.
Kaka A. 2020. Karakteristik dan Daya Fertilitas Spermatozoa Babi Peranakan Landrace. J. Pet. Indon. 22(3): 277-283.
Lee E, Kim D. 2018. Effects of astaxanthin on miniature pig sperm cryopreservation. Bio. Med. Res. Int. 2018: 6784591.
Lewis SE, Aitken RJ. 2005. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Res. 322(1): 33-41.
Mariana Y dan Alimuddin. 2020. Penambahan Level Ekstrak Wortel (Daucus carota) pada Pengencer Andromed Dalam Mempertahankan Kualitas Spermatozoa Sapi Bali pada Suhu 5OC. JSTL. 6(2): 241-248.
Martemucci G, Costagliola C, Mariano M, D’andrea L, Napolitano P and D’Alessandro AG, 2022. Free radical properties, source and targets, antioxidant consumption and health. Oxygen. 2(2): 48-78.
Meneses PJS, Alejandro JC, Vidal M, Ana JS, José JG, María RFS. 2019. Ovine sperm DNA oxidation quantification using an 8‐OHdG immunodetection assay. Repord. Dom. Anim. 54 (4):59-64.
Nahak PL, Agustinus AD, Kristofus WK. Kualitas Semen Babi Landrace Dalam Pengencer Semen Sitrat-Kuning Telur yang Ditambah Glukosa Dengan Konsentrasi Berbeda. J. Anim. Sci. 7(1): 12-15.
Nakajima H, Unoda K, Ito T, Kitaoka H, Kimura F, Hanafusa T. 2012. The relation of urinary 8-OHdG, a marker of oxidative stress to dna, and clinical outcomes for ischemic stroke. Open Neurol. J. 6: 51−57.
Nakao T, Sugihashi A, Kawata K, Saga N, Tsunoda N. 1983. Milk progesterone levels in cows with normal or prolonged estrous cycles, referenced to an early pregnancy diagnosis. Japanese J. Vet. Sci. 45(4): 495-499.
Pereira CPM, Souza ACR, Vasconcelos AR, Prado PS, Name JJ. 2021. Antioxidant and anti-inflammatory mechanisms of action of astaxanthin in cardiovascular diseases (Review). Int. J. Mol. Med. 47: 37-48.
Pérez-Gálvez A, Viera I, Roca M. 2020. Carotenoids and chlorophylls as antioxidants. Antioxidants 9(6): 505.
Prihantoko KD, Yuliastuti F, Haniarti H, Kusumawati A, Widayati DT, Budiyanto A. 2020. The effect of genistein on the plasma membrane integrity of frozen ongole grade bull semen based on skim milk-soy lecithin extender. IOP Conf. Series: Earth Environ. Sci. 465: 012054.
Rizal M. 2020. Diseminasi teknologi inseminasi buatan menggunakan semen kambing peranakan etawa (PE) dengan pengencer air kelapa muda dan kuning telur di Kecamatan Bati Bati Kabupaten Tanah Laut Kalimantan. Panrita Abadi. 1(2): 56-61.
Robertis ED, Robertis EM. 1979. Cell and Moleculer Biology. Philadelphia: Saydesr College.
Shipley CF. 1999. Breeding sounders examination of the boar. Swine Health Prod. 7(3): 117-120.
Sikka SC. 1996. Oxidative stress and role of antioxidant in normal and abnormal sperm function. Front. Biosci. 1: e78-86.
Solihati NR, Rasad SD, Rizal MZ, Fitriati M. 2008. Kualitas spermatozoa cauda epididymis sapi peternakan ongole (PO) dalam pengencer sus, tris dan sitrat kuning telur pada penyimpanan 4-5oC. Anim. Prod. 10(1): 22-29.
Sumardani NLG, Komang B, Tjok IP, Antonius WP. 2019. Umur Memengaruhi Volume Semen dan Motilitas Spermatozoa Babi Landrace di Balai Inseminasi Buatan Baturiti, Tabanan, Bali. J. Vet. 20(3): 324-329.
Vassilev N, Yotov S, Dimitrov F. 2005. Incidence of early embryonic death indairy cows. Trakia J. Sci. 3(5): 62-64.
Yulnawati, Maheshwari H, Rizal M, Herdis. 2010. Maltosa Mempertahankan Viabilitas Spermatozoa Epididimis Kerbau Belang yang Disimpan dalam Bentuk Cair. J. Vet. 11(1): 126-130.
Aritonang. 1993. Beternak babi. Penerbit Mutiara, Jakarta.
Awda, Basim J, Mackenzie-Bel Ml, Marry M, Buhr. 2009. Reactive Oxygen Species and Boar Sperm Function. Biol. Reprod. 8: 553-561.
Bebas W, Agustina KK. 2022. Short Communication: The use of lactose-astaxanthin to maintain the quality of Green junglefowl frozen semen. Biodiversitas. 23(11): 5759-5764.
Bebas W, Gorda IW. 2020. The best level of glycerol and dimethylsulfoxide cryoprotectant in the astaxanthin phosphate duck egg yolk extender on the quality of boar frozen semen. J. Vet. 21(1): 115-123.
Bebas W, Geovany LB, Made KB. 2016. Penambahan Vitamin E Pada Pengencer BTS® Terhadap Daya Hidup Dan Motilitas Spermatozoa Babi Landrace Pada Penyimpanan 15° C. Bul. Vet. Udayana. 8(1): 1-7.
Breininger E, Beorlegui NB, OFlaherty CM. 2004. Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen. Theriogenol. 63: 2126- 2135.
Brotosudarmo THP, Limantara L, Setiyono E, Heriyanto. 2020. Structures of astaxanthin and their consequences for therapeutic application. Int. J. Food Sci. 2020: 2156582.
Budaarsa K, Puger AW, Suasta IM. 2016. Ekplorasi komposisi pakan tradisional babi bali. Maj. Ilmiah Pet. 19(1): 6-11.
Chatterjee S, De Lamirande, Gagnon E. 2001. Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: protection by oxidized glutathione. Mol. Reprod. Dev. 60: 498-506.
Dethan AA, Kustono, Hartadi H. 2010. Kualitas dan Kuantitas Sperma Kambing Bligon Jantan yang Diberi Pakan Rumput Gajah dengan Suplementasi Tepung Darah. Bul. Pet. 34(3): 145-153.
Dose J, Matsugo S, Yokokawa H, Koshida Y, Okazaki S, Seidel U, Eggersdorfer M, Rimbach G, Esatbeyoglu T. 2016. Free radical scavenging and cellular antioxidant properties of astaxanthin. Int. J. Mol. Sci. 17(1): 103.
Feka WV, Dethan AA, Beyleto VY. 2016. Pengaruh Lama Penyimpanan terhadap Viabilitas dan pH Semen Babi Landrace yang Diencerkan Menggunakan Bahan Pengencer Sitrat Kuning Telur. JAS. 1(3): 34-35.
Gadea J. 2003. Semen extenders used in the artificial insemination of swine. Spanish J of Agri Research. 1(2): 17-27.
Garner DL, Hafez ESE. 2000. Spermatozoa and seminal plasma. In: Hafez B. Hafez ESE, editor. Reproduction in Farm Animals. 7th Ed. Philadelphia (US): Lippincott Williams and Wilkins. Pp. 96-109.
Guo HT, Wang JR, Sun LZ, Jin XH, Shi XY, Lin JY, Yue SL, Zhou JB. 2021. Effects of astaxanthin on plasma membrane function and fertility of boar sperm during cryopreservation. Theriogenol. 164: 58- 64.
Hartono M. 2008. Optimalisasi penambahan vitamin E dalam pengencer sitrat kuning telur untuk mempertahankan kualitas semen kambing boer. J. Indon. Trop. Anim. Agric. 33: 1-9.
Hofer T. 2001. Method Development of Analysis of 8-oxodG as a Biomarker for Oxidative Stress. Departemen Biosains: Institut Karolinska.
Hsieh YY, Chang CC, Lin CS. 2006. Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility. Int. J. Biol. Sci. 2: 23-29.
Johnson LA, Weitze KF, Fiser P, Maxwell WMC. 2000. Storage of boar semen. J. Anim. Sci. 62: 143-172.
Juan CA, Pérez de la Lastra JM, Plou FJ and Pérez-Lebeña E, 2021. The chemistry of reactive oxygen species (ROS) Revisited: outlining their role in biological macromolecules (DNA, Lipids and Proteins) and induced pathologies. Int. J. Mol. Sci. 22(9): 4642.
Kaka A. 2020. Karakteristik dan Daya Fertilitas Spermatozoa Babi Peranakan Landrace. J. Pet. Indon. 22(3): 277-283.
Lee E, Kim D. 2018. Effects of astaxanthin on miniature pig sperm cryopreservation. Bio. Med. Res. Int. 2018: 6784591.
Lewis SE, Aitken RJ. 2005. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Res. 322(1): 33-41.
Mariana Y dan Alimuddin. 2020. Penambahan Level Ekstrak Wortel (Daucus carota) pada Pengencer Andromed Dalam Mempertahankan Kualitas Spermatozoa Sapi Bali pada Suhu 5OC. JSTL. 6(2): 241-248.
Martemucci G, Costagliola C, Mariano M, D’andrea L, Napolitano P and D’Alessandro AG, 2022. Free radical properties, source and targets, antioxidant consumption and health. Oxygen. 2(2): 48-78.
Meneses PJS, Alejandro JC, Vidal M, Ana JS, José JG, María RFS. 2019. Ovine sperm DNA oxidation quantification using an 8‐OHdG immunodetection assay. Repord. Dom. Anim. 54 (4):59-64.
Nahak PL, Agustinus AD, Kristofus WK. Kualitas Semen Babi Landrace Dalam Pengencer Semen Sitrat-Kuning Telur yang Ditambah Glukosa Dengan Konsentrasi Berbeda. J. Anim. Sci. 7(1): 12-15.
Nakajima H, Unoda K, Ito T, Kitaoka H, Kimura F, Hanafusa T. 2012. The relation of urinary 8-OHdG, a marker of oxidative stress to dna, and clinical outcomes for ischemic stroke. Open Neurol. J. 6: 51−57.
Nakao T, Sugihashi A, Kawata K, Saga N, Tsunoda N. 1983. Milk progesterone levels in cows with normal or prolonged estrous cycles, referenced to an early pregnancy diagnosis. Japanese J. Vet. Sci. 45(4): 495-499.
Pereira CPM, Souza ACR, Vasconcelos AR, Prado PS, Name JJ. 2021. Antioxidant and anti-inflammatory mechanisms of action of astaxanthin in cardiovascular diseases (Review). Int. J. Mol. Med. 47: 37-48.
Pérez-Gálvez A, Viera I, Roca M. 2020. Carotenoids and chlorophylls as antioxidants. Antioxidants 9(6): 505.
Prihantoko KD, Yuliastuti F, Haniarti H, Kusumawati A, Widayati DT, Budiyanto A. 2020. The effect of genistein on the plasma membrane integrity of frozen ongole grade bull semen based on skim milk-soy lecithin extender. IOP Conf. Series: Earth Environ. Sci. 465: 012054.
Rizal M. 2020. Diseminasi teknologi inseminasi buatan menggunakan semen kambing peranakan etawa (PE) dengan pengencer air kelapa muda dan kuning telur di Kecamatan Bati Bati Kabupaten Tanah Laut Kalimantan. Panrita Abadi. 1(2): 56-61.
Robertis ED, Robertis EM. 1979. Cell and Moleculer Biology. Philadelphia: Saydesr College.
Shipley CF. 1999. Breeding sounders examination of the boar. Swine Health Prod. 7(3): 117-120.
Sikka SC. 1996. Oxidative stress and role of antioxidant in normal and abnormal sperm function. Front. Biosci. 1: e78-86.
Solihati NR, Rasad SD, Rizal MZ, Fitriati M. 2008. Kualitas spermatozoa cauda epididymis sapi peternakan ongole (PO) dalam pengencer sus, tris dan sitrat kuning telur pada penyimpanan 4-5oC. Anim. Prod. 10(1): 22-29.
Sumardani NLG, Komang B, Tjok IP, Antonius WP. 2019. Umur Memengaruhi Volume Semen dan Motilitas Spermatozoa Babi Landrace di Balai Inseminasi Buatan Baturiti, Tabanan, Bali. J. Vet. 20(3): 324-329.
Vassilev N, Yotov S, Dimitrov F. 2005. Incidence of early embryonic death indairy cows. Trakia J. Sci. 3(5): 62-64.
Yulnawati, Maheshwari H, Rizal M, Herdis. 2010. Maltosa Mempertahankan Viabilitas Spermatozoa Epididimis Kerbau Belang yang Disimpan dalam Bentuk Cair. J. Vet. 11(1): 126-130.
Published
2023-07-15
How to Cite
SANJAYA, Gede Putra et al.
ADDITION OF VITAMIN E IN SEMENLIFE® DILUENT ON QUALITY AND SHELF LIFE OF SPERMATOZOA OF LANDRACE PIGS.
Buletin Veteriner Udayana, [S.l.], p. 1170-1180, july 2023.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/103216>. Date accessed: 27 apr. 2024.
doi: https://doi.org/10.24843/bulvet.2023.v15.i06.p16.
Section
Articles
