HISTOLOGY OF THE ABDOMEN SKIN AND LEUKOCYTES PROFILE OF DOGS WITH DERMATITIS
Abstract
Skin is the markers of dog health and damage/lesions on the skin cause the dog appearance to be unattractive. One of the diseases that affect the appearance of dogs is dermatitis. Dermatitis is an infection that attacks the skin organs and tends to be difficult to cure, especially infections that occur in the abdominal area. Therefore, in its treatment, it is necessary to know the level of changes in skin lesions, as well as the patient's blood profile. This study aims to determine the histology of the skin and the total leukocyte profile of dogs with dermatitis and compared with non-dermatitis. 24 samples were taken from dogs with dermatitis and non-dermatitis. The samples form abdominal skin tissue and whole blood were taken by purposive sampling method. Histological images were examined using a microscope (400x) with the Haris haematoxilin eosin staining method, while the total leukocyte profile was measured using a hematology analyzer. The results showed that the clinical symptoms of dogs suffering from dermatitis were characterized by: itching, redness/rubor, hair loss/alopecia. Epidermal skin histology found: hyperkeratosis of the stratum corneum, necrosis, inflammatory cell, hydrophic and spogiotic degeneration of keratocyte cells, hyperplasia of the stratum granulosum. There is a segment s. scabiei in hair follicles, infiltration of lymphocytes and neutrophils, folliculitis and furunculosis in the dermis and hypodermis layers. While the leukocyte profile was found to increase, namely in dogs with dermatitis as much as 59% and non-dermatitis 50%. It can be concluded that dogs with dermatitis experience changes in histology and total leukocytes. However, in the future studies it is advisable to classify the types of dermatitis and differential leukocytes.
Downloads
References
Ali MH, Begum N, Azam MG, Roy BC. 2011. Prevalence and Pathology Of Mite Infestation In Street Dogs At Dinajpur Municipality Area. J. Bangladesh Agril. Univ. 9(1): 111–119.
Ambily VR, Usha NP, Ajithkumar S, Deepa C, Vinu David P. 2022. A prospective study on haemato-biochemical aspects of atopic dermatitis in dogs. Department of Clinical Veterinary Medicine. Kerala Veterinary and Animal Sciences University, Kerala. India.
Berata IK, Winaya IBO, ADI AAAM, Adnyana IBW. 2019. Buku Ajar Patologi Veteriner Umum. Cetakan Ke-5. Denpasar: Swasta Nulus.
Besung INK, Suwiti NK, Mahardika IGNK, Suardana IW. 2023. Jamur dan Penyakit Yang Timbul pada Hewan. Cetakan Ke-I. Media Nusa Creative.
Bourguignon JP, Giudiece LC. 2013. Endocrine-disrupting chemicals. An Endocrine Society Scientific Statement. Endocrine Rev. 30: 293-342
Desmawati. 2013. Sistem Hematologi dan Imunologi. Edited by D. Juliastuti. Penerbit In Media. Jakarta.
Dharmawan NS. 2002. Buku Ajar Pengantar Patologi Klinik Veteriner, Hematologi Klinik 2nd ed. Denpasar.
Elisa B, Guimar LD, Ferreira TS, Favarato ES. 2013. Chapter 1: Dermatology in Dogs and Cats. Licensee InTech.
Fan YK, Hsu JC, Peh HC, Tsang CL, Cheng SP, Chiu SC, Ju JC. 2002. The Effects of Endurance Training on the Hemogram of the Horse. Asian-Australasian J. Anim. Sci. 15(9):1348-1353.
Ferrer L, Raverta I, Silbermayr K. 2014. Immunology and pathogenesis of canine demodicosis. J. Vet. Sci. 3(71): 1324-1331.
Ferreira TC, Rodrigeus FR, Lopes CEB, de Matos MG. 2017. Can Pagoda Red staining be used for histopathological differentiation of canine allergic dermatitis. Revist. Brasileira de Higiene a Sanidade Anim. 11(3): 235-262.
Founda MA, Saeed HA, Abdelgayed SS, Abdou OM. 2021. Clinical Haemato-biochemical and Histopathological Studies on some Dermopathies in Dogs. Faculty of Veterinary Medicine. Egypt.
Harlim. 2016. Buku Ajar Ilmu Kesehatan Kulit dan Kelamin. Fakultas Kedokteran Universitas Kristen Indonesia. Jakarta
Hartono M, Elisa E, Siswanto S, Suharyati S, Santosa PE, Sirat MMP. 2019. Profil Darah pada Sapi Simmental-Peranakan Ongole Akibat Infestasi Cacing Trematoda di Desa Labuhan Ratu, Kecamatan Labuhan Ratu, Kabupaten Lampung Timur, Provinsi Lampung. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. Jember.
Hoskova Z, Svoboda M, Satinska D, Matiasovic J, Leva L, Toman M. 2015. Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis. Vet. Med. 60, 2015 (11): 644–653.
James WD, Berger TG, Elston DM. 2016. Bacterial infections. In: Andrews’ Diseases of the skin. Clinical Dermatology. 12th Ed. Philadelphia: Elsevier. 254–5.
Kalangi SJR. 2013. Histofisiologi Kulit. J. Biom. 5(3): 12-20.
Kurniawati NMA, Setiasih NLE, Suastika P. 2020. Struktur Histologi dan Histomorfometri Kulit Anjing Ras Kintamani Asal Bali. J. Vet. 21(4): 646-653.
Kim HJ, Choi EJ, Lee HR, Park GJ, Yun ES, Kim JH, Do SH. 2015. Spindle Cell Limpoma in The Gingva of A Dog: A Case Report. Vet. Med. 60(6): 336-340.
Medleau L, Hnilica KA. 2006. Small Animal Dermatologi: A Colour Atlas and Therapheutic Guide. 2nd Edition. St. Louis Missouri. Elsevier. USA.
McWilliam AS, Napoli S, Marsh AM, Pemper FL, Nelson DJ, Pimm CL, Stumbles PA, Wells TN. 1996. Dendritic cells are recruited into the airway epithelium during the inflammatory response to a broad spectrum of stimuli. J. Exp. Med. 184:2429.
Nemeth NMM, Ruder G, Gerhold RW, Brown JD, Munk BA, Oestrele PT, Kubiski SV, Keel MK. 2014. Demodectic Mange, Dermatophilosis and Other Parasitic and Bacterial Dermatologic Disease In Free Ranging White-Tailed Deer (Odocoileus Virginianus) In The United State. Vet. Pathol. 51(3): 633-640.
Putra PDP, Suwiti NK, Susari NNW. 2022. Struktur Histologi Kulit Bagian Ekstremitas Caudal, Dorsum, dan Abdomen Anjing Penderita Dermatitis. Buletin Veteriner Udayana.
Radityastuti dan Anggraeni P. 2017. Karakteristik Penyakit Kulit Akibat Infeksi Di Poliklinik Kulit dan Kelamin RSUP Dr Kariadi Semarang. Medical Faculty of Diponegoro University.
Sharma S, Pokharel S. 2019. Diagnosis and Therapeutic Management of Mixed Demodex and Sarcoptes Mite Infestation in Dog. Acta Scient. Agric. 3(6): 163-166.
Scott DW, Miller W. 2011. Diagnostic Methods. Equine Dermatology (Second Edition). Elsevier.
Sudira IW, Purba DJ, Dharmawan NS. 2018. Gambaran Leukosit Anak Anjing Kintamani yang Diberikan Kapsul Temulawak dan Divaksin Rabies. Indon. Med. Vet. 7(4): 367-376.
Suwiti NK, Suastika IP, Swacita IBN, Besung INK. 2015. Studi Histologi dan Hitomorfometri Daging Sapi Bali dan Wagyu. J. Vet. 3(16): 432-438.
Suwiti NK, Windhu M, Watiniasih NL, Besung INK, Suartha IN. 2018 The Expression Of Cd4+ Lymphocytes Of Bali Cattle After Consuming Mixed Minerals. J. Adv.Trop. Biodiv. Environ. Sci. 1(2): 2549-6980.
Tanei R, Hasegawa Y. 2022. Immunological Pathomechanisms of Spongiotic Dermatitis in Skin Lesions of Atopic Dermatitis. Int. J. Mol. Sci. 23 (6682): 2-29.
Weiss DJ, Wardrop KJ. 2010. Weiss dan Wardrop, 2010’s Veterinary Hematology. 6th Edition. Iowa: Wiley-Blackwell Publishing.
Widyanti AI, Suartha IN, Erawan IGMK, Anggreni LD, Sudimartini LM. 2018. Hemogram Anjing Penderita Dermatitis Kompleks. Indon. Med. Vet. 7(5): 576-587.
Widyastuti SK, Dewi NMS, Utama IH. 2012. Kelainan Kulit Anjing Jalanan pada Beberapa Lokasi di Bali. Bul. Vet. Udayana. 4(2): 81-86.