KONSERVASI ANGGREK ALAM INDONESIA Vanda tricolor Lindl. varietas suavis MELALUI KULTUR EMBRIO SECARA IN-VITRO
Abstract
Vanda tricolor Lindl. var. suavis is an Indonesian wild orchid which is now extremely rare in nature due to its habitat destruction. Development of an appropriate method for propagation of this species through in vitro culture could be nessecary for conservation purposes. High phenolic content of plant tissue is a serious problem for V. tricolor research in the laboratory, which inhibits germination and growth of the embryo. To overcome this problem, seeds were sown in a medium with the addition of tomato extract as an antioxidant. The aim of this research is to find the most suitable concentration of tomato extract for germination and growth of the embryo of V. tricolor form Bali and Merapi in order to obtained healthy seedlings for conservation purposes. Orchid pods (7 months after polination) of V. tricolor Bali and Merapi were used as plant material. The treatment consisted of 5 concentrations of tomato extract which is 0, 50 100, 150, 200, 250 g L-1. Observation was done by counting the number of protocorms for each stage of growth at 4 weeks after seed sowing. The study concluded that V. tricolor Bali is more responsive to the tomato extract compared with Merapi. Concentration of 150 gL-1 tomato extract gave the highest percentage of protocorms for Bali form, whereas Merapi form did not give significant differences either with or without tomato extract added in the culture medium.Downloads
Download data is not yet available.
Published
2012-02-01
How to Cite
DWIYANI, Rindang et al.
KONSERVASI ANGGREK ALAM INDONESIA Vanda tricolor Lindl. varietas suavis MELALUI KULTUR EMBRIO SECARA IN-VITRO.
Bumi Lestari, [S.l.], v. 12, n. 1, p. 93-98, feb. 2012.
ISSN 2527-6158.
Available at: <https://ojs.unud.ac.id/index.php/blje/article/view/1539>. Date accessed: 13 nov. 2024.
Issue
Section
Original Research Articles
Keywords
germination; tomato extract; protocorm; V. tricolor; forma Bali; forma Merapi
Authors who publish with this journal agree to the following terms:
- All articles published by Bumi Lestari Journal of Environment and Environmental Reseach Center Udayana University are made available under an open access license worldwide immediately. This means everyone has free and unlimited access to the full-text of all articles published in Bumi Lestari Journal of Environment, and everyone is free to re-use the published material given proper accreditation/citation of the original publication. Open access publication is supported by authors' institutes or research funding agency by payment of a comparatively article processing charge for accepted articles (See Author Fees). Bumi Lestari Journal of Environment and Environmental Reseach Center Udayana University publish articles under the Creative Commons Attribution License.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).