First Molecular Identification of Sunfish in North Bali Water
Abstract
Stranded Sunfish in North Bali with full body we collect to do molecular identification. Samples were amplified at the d-loop locus (control region) using the PCR (Polymerase Chain Reaction) method. Primers used in PCR are H16498 as primary front (forward) and L15812 as reverse primer. Similarity value of 95% after alignment with Mola ramsayi (accession number accession AY940824) on GenBank, and the gaps of the nucleotide just 1%. The stranded sunfish identified using partial sequence mtDNA is the same species as the species Mola ramsayi.
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References
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[17] Thys, Tierney M., Whitney, Jonathan, Hearn, Alex, Weng, Kevin C., Peñaherrera, Caesar, Jawad, L., Alfaro-Shigueto, J., Mangel, J.C., Karl, Stephen A. 2013. First record of the southern ocean sunfish, Mola ramsayi, in the Galapagos Marine Reserve. Marine Biodiversity Records. 6:1-4.
[18] Thys, Tierney, Ryan, John P., Weng, Kevin C., Erdmann, Mark, Tresnati, Joeharnani. 2016. Tracking a Marine Ecotourism Star: Movements of the Short Ocean Sunfish Mola ramsayi in Nusa Penida, Bali, Indonesia. Journal of Marine Biology.
[19] Tyler, J.C. 1970. The progressive reduction in number of elements supporting the caudal fin of fishes of the order Plectognathi. Proc Acad Nat Sci Phila. 122. 1-85.
[20] Walsh, P., D.A. Metzger, and R. Higuchi. 1991. Chelex-100 as a medium for simple extraction of DNA for PCR based typing from forensic material. Biotechniques, 10:506-513.
[21] Yoshita, Yukiko, Yamanoue, Yusuke, Sagara, Kotaro, Nishibori, Masahide, Kuniyoshi, Hisato, Umino, Tetsuya, Yoichi Sakai, Hiroaki, Hashimoto, Gushima, Kenji. 2009. Phylogenetic relationship of two Mola sunfishes (Tetraodontiformes: Molidae) occurring around the coast of Japan, with notes on their geographical distribution and morphological characteristics. Ichthyol Res. 56:232-244.
[2] Dewar, H., Thys, T., Teo, S. L. 2010. Satellite tracking the world’s largest jelly predator, the ocean sunfish, Mola mola, in the Western Pasific. Journal of Experimental Marine Biology and Ecology, 393(1-2): 32-42.
[3] Fraser-Brunner, A. 1951. The ocean sunfishes (family Molidae). Bulletin of the British Museum (Natural History), Zoology. 1: 87–121.
[4] Houghton, Jonathan D.R., Doyle, Thomas K., Davenport, John, and Hays, Graeme C. The ocean sunfish Mola mola: insights into distribution, abundance and behavior in the Irish and Celtic Seas. J. Mar. Biol. 86:1237-1243.
[5] Lee, WJ., Conroy J, Howell WH., Kocher TD. 1995. Structure and evolution of teleost mitochondrial control regions. J Mol Evol. 41:54-66.
[6] Potter, I. F., and Howell, W. H. 2011. Vertical movements and behavior of the ocean sunfish, Mola mola in the Northwest Atlantic. Marine Biology. 158(3):531-540.
[7] Rosel, P.E., A.E. Dizon, and J.E. Heyning. 1994. Genetic analysis of sympatric morphotypes of common dolphins (genus Delphinus). Marine Biology, 119(2):159-167.
[8] Santini, Francesco, and Tyler, James C. 2003. A phylogeny of the families of fossil and extant teraodontiform fishes (Acanthomorpha, Tetraodontiformes), Upper Cretaceus to Recent. Zoological Journal of the Linnean Society. 139:565-617.
[9] Sawai, Etsuro, Yamanoue, Yasuke, Jawad, Laith, Al-Mamry, Juma, and Sakai, Yoichi. 2017a. Molecular and Morphological Identification of Mola Sunfish Specimens (Actinopterygii: Tetraodontiformes: Molidae) from the Indian Ocean. Species Diversity. 22:99-104.
[10] Sawai, Etsuro, Yamanoue, Yasuke, Nyegaard, Marianne, and Sakai, Yoichi. 2017b. Redescription of the bump-head sunfish Mola alexandrine (Ranzani 1839), senior synonym of Mola ramsayi (Giglioli 1883), with designation of a neotype for Mola mola (Linnaeus 1758) (Tetraodontiformes: Molidae).
[11] Smith MM, Heemstra PC.1986. Smith’s Sea Fishes, 6th edn. Springer, Berlin Heidelberg New York.
[12] Sneath P.H.A. and Sokal R.R. 1973. Numerical Taxonomy. Freeman, San Francisco.
[13] Tamura K., Nei M., and Kumar S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA). 101:11030-11035.
[14] Tamura K., Stecher G., Peterson D., Filipski A., and Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution. 30: 2725-2729.
[15] Thys, Tierney M., Hearn, Alex R., Weng, Kevin C., Ryan, John P., and César Peñaherrera-Palma. 2017. Satellite tracking and site fidelity of short ocean sunfish, Mola ramsayi, in the Galapagos Islands. Journal of Marine Biology.
[16] Thys, Tierney M., Ryan, John P., Dewar, Heidi, Perle, Christopher R., Lyons, Kady, O’Sullivan, John, Farwell, Charles, Howard, Michael J., Weng, Kevin C., Lavaniegos, Bertha E., Gaxiola-Castro, Gilberto, Bojorquez, Luis Erasmo Miranda, Hazen, Elliot L., Bograd, Steven J. 2015. Ecology of the Ocean Sunfish, Mola mola, in the southern California Current System. Journal of Experimental Marine Biology and Ecology. 471:64-76.
[17] Thys, Tierney M., Whitney, Jonathan, Hearn, Alex, Weng, Kevin C., Peñaherrera, Caesar, Jawad, L., Alfaro-Shigueto, J., Mangel, J.C., Karl, Stephen A. 2013. First record of the southern ocean sunfish, Mola ramsayi, in the Galapagos Marine Reserve. Marine Biodiversity Records. 6:1-4.
[18] Thys, Tierney, Ryan, John P., Weng, Kevin C., Erdmann, Mark, Tresnati, Joeharnani. 2016. Tracking a Marine Ecotourism Star: Movements of the Short Ocean Sunfish Mola ramsayi in Nusa Penida, Bali, Indonesia. Journal of Marine Biology.
[19] Tyler, J.C. 1970. The progressive reduction in number of elements supporting the caudal fin of fishes of the order Plectognathi. Proc Acad Nat Sci Phila. 122. 1-85.
[20] Walsh, P., D.A. Metzger, and R. Higuchi. 1991. Chelex-100 as a medium for simple extraction of DNA for PCR based typing from forensic material. Biotechniques, 10:506-513.
[21] Yoshita, Yukiko, Yamanoue, Yusuke, Sagara, Kotaro, Nishibori, Masahide, Kuniyoshi, Hisato, Umino, Tetsuya, Yoichi Sakai, Hiroaki, Hashimoto, Gushima, Kenji. 2009. Phylogenetic relationship of two Mola sunfishes (Tetraodontiformes: Molidae) occurring around the coast of Japan, with notes on their geographical distribution and morphological characteristics. Ichthyol Res. 56:232-244.
Published
2019-06-26
How to Cite
RIAWAN, I Made Oka et al.
First Molecular Identification of Sunfish in North Bali Water.
Advances in Tropical Biodiversity and Environmental Sciences, [S.l.], v. 3, n. 1, p. 12-16, june 2019.
ISSN 2622-0628.
Available at: <https://ojs.unud.ac.id/index.php/atbes/article/view/49700>. Date accessed: 19 apr. 2024.
doi: https://doi.org/10.24843/ATBES.2019.v03.i01.p04.
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