Kebuntingan Hasil Transfer Blastosis Mencit yang Dibekukan dengan Metode Vitrifikasi Kriolup

  • I Wayan Batan
  • I Ketut Suatha
  • Ita Djuwita
  • Nining Handhayani
  • Wahono Esti Prasetyaningtyas
  • Ketut Adnyane Mudite
  • Bibiana W Lay
  • Supar -
  • Arief Boediono

Abstract

The aim of the study was to assess the viability of vitrified embryo using cryoloop as a carrier ofembryo. The blastocyst stage embryos were collected from superovulated mice. Embryos were frozenusing vitrification method and vitrified embryos were loaded on copper filament cryoloop before dipped inliquid nitrogen. The viability of vitrified embryos was assess in vitro by medium cultered and in vivo bytransfered them to recipient mice. The result shows the viability of vitrified embryos was 85,7% after 24hours cultured and the embryos were born from two pregnant recipient mice out of nine (22%) or fouroffspring out of 63 trasfered embryos (6%). In conclusion, vitrified blatocyst stage embryos using cryoloopas a carrier could keep the viability of the embryos and they could be transfered to the recipient mice andwere born normally.

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Author Biographies

I Wayan Batan
Lab Diagnosis Klinik Hewan,Fakultas Kedoktern Hewan, Universitas Udayana,Jl. Raya Sesetan Gang Markisa No. 6 Denpasar
I Ketut Suatha
Lab Anatomi,Fakultas Kedoktern Hewan, Universitas Udayana,Jl. Raya Sesetan Gang Markisa No. 6 Denpasar
Ita Djuwita
FKH Institut Pertanian Bogor, Dramaga, Bogor
Nining Handhayani
FKH Institut Pertanian Bogor, Dramaga, Bogor
Wahono Esti Prasetyaningtyas
FKH Institut Pertanian Bogor, Dramaga, Bogor
Ketut Adnyane Mudite
FKH Institut Pertanian Bogor, Dramaga, Bogor
Bibiana W Lay
FKH Institut Pertanian Bogor, Dramaga, Bogor
Supar -
Lab Mikrobiologi, Balai Besar Penelitian Veteriner, Cimanggu, Bogor
Arief Boediono
FKH Institut Pertanian Bogor, Dramaga, Bogor
How to Cite
BATAN, I Wayan et al. Kebuntingan Hasil Transfer Blastosis Mencit yang Dibekukan dengan Metode Vitrifikasi Kriolup. Jurnal Veteriner, [S.l.], p. 185-191, nov. 2012. ISSN 2477-5665. Available at: <http://ojs.unud.ac.id/index.php/jvet/article/view/3508>. Date accessed: 12 sep. 2025.
Citation Formats
Issue
Vol 12, No 3 (2011)
Section
Articles

Keywords

embryo trasfer, blastocyst, vitrification, cryoloop
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License
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