Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton

  • Asmarani Kusumawati Fakultas Kedokteran Hewan, Universitas Udayana, Bali
  • Atik Ratnawati
  • Ida Arlita Wulandari
  • Sri Hartati
  • Tri Untari

Abstract

Jembrana disease is an infectious disease in Bali cattle cause by a member of lentivirus calledjembrana disease virus (JDV). It causes an acute and severe disease syndrome with short incubationperiod. As the disease has spread to several areas in Indonesia, a simple and rapid detection method isrequired. The objective of this study to apply rapid diagnostic method for JDVTabanan 1995 strain basedon Nucleic Acid Sequence Based Amplification (NASBA) methods targeting env-tm gene. The steps of thisresearch consisted of viral RNA isolation from organ and blood of cattle experimentaly infected withJDVTabanan 1995 strain . RNA amplification was conducted by NASBA using waterbath. The NASBAproducts were then separated on 2 % agarose gel. Using this technique JDV positive result was obtainedfrom organ samples such as spleen, liver, lung, prefemoralis lymph node, prescapularis lymph node andblood generating a RNA fragment of 207 bp. In this study, diagnosis method for env tm of JDV Tabanan1995 strain can be conducted by isothermal amplification NASBA.

Downloads

Download data is not yet available.
Published
2016-01-05
How to Cite
KUSUMAWATI, Asmarani et al. Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton. Jurnal Veteriner, [S.l.], v. 16, n. 4, p. 569-575, jan. 2016. ISSN 2477-5665. Available at: <http://ojs.unud.ac.id/index.php/jvet/article/view/17340>. Date accessed: 04 sep. 2025.
Citation Formats
Issue
Vol 16 No 4 (2015)
Section
Articles

Keywords

Bali cattle; NASBA; env-TM gene; JDV; Tabanan 1995 strain
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License
Warning: array_merge(): Argument #2 is not an array in /var/www/ojs.unud.ac.id_backup/lib/pkp/classes/core/PKPApplication.inc.php on line 578 Warning: Invalid argument supplied for foreach() in /var/www/ojs.unud.ac.id_backup/plugins/generic/recommendByAuthor/RecommendByAuthorPlugin.inc.php on line 114

Most read articles by the same author(s)