EFFECT OF VITAMIN C ON THE BLOOD PROFILE OF MALE RATS (Rattus norvegicus) INDUCED BY SODIUM NITRITE (NaNO2)

  • Galih Anindita Kusumatjahja Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Udayana
  • Inna Narayani Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Udayana
  • I Made Sara Wijana Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Udayana

Abstract

Sodium nitrite (NaNO2) is a chemical compound that is often used in food industry. NaNO2 widely used for food preservatives, mainly in processed meat products. However, NaNO2 could cause anemia by interfering with the process of transporting oxygen in the blood. The effects caused by NaNO2 could be repaired with the use of vitamin C. This study was aimed to determine the effect of vitamin C on the blood profile of rats induced by NaNO2, and the effective dose to improve the blood profile of rats. The study used a completely randomized design (CRD) with 4 groups, namely K- (Aquades); K+ (NaNO2 37,5mg/kgBW); C1 (NaNO2 37,5 mg/kgBW + vitamin C 1,8 mg/animal/day); C2 (NaNO2 37,5 mg/kgBW + vitamin C 3,6 mg/animal/day). The samples used in each treatment group were 6 rats, therefore the total sample used was 24 male white rats (Rattus norvegicus) strain Wistar. NaNO2 and vitamin C solutions were administered orally by the gavage method. NaNO2 solution was given until 35th day, then vitamin C solution was given on 22nd to 35th day. Blood samples were taken three times, the day before the administration of NaNO2, on the 21st day, and on the 36th day, further analysis of blood used a haematology analyzer and blood smears preparations Giemsa staining. Data were statistical analyzed by ANOVA and continued with Duncan's test. The results showed that Vitamin C can improve the blood profile, with the most effective dose of vitamin C is 1,8 mg/animal/day.

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Published
2022-09-02
How to Cite
KUSUMATJAHJA, Galih Anindita; NARAYANI, Inna; WIJANA, I Made Sara. EFFECT OF VITAMIN C ON THE BLOOD PROFILE OF MALE RATS (Rattus norvegicus) INDUCED BY SODIUM NITRITE (NaNO2). SIMBIOSIS, [S.l.], v. 10, n. 2, p. 186-198, sep. 2022. ISSN 2656-7784. Available at: <https://ojs.unud.ac.id/index.php/simbiosis/article/view/82714>. Date accessed: 31 jan. 2023. doi: https://doi.org/10.24843/JSIMBIOSIS.2022.v10.i02.p06.
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Articles