Isolasi dan Identifikasi Bakteri Selulolitik pada Buah Kopi Arabika (Coffea arabica L.)
Abstract
Cellulolytic bacteria are microorganisms that can produce cellulase enzymes. Cellulolytic bacteria contain cellulase enzymes that play a role in the cellulose degradation processes. The purpose of this study was to isolate cellulolytic bacteria that capable to degrading cellulose from arabica coffee cherries collected from Catur Village, Kintamani. The results of bacterial isolation at 10-6 and 10-8 dilutions obtained 10 bacterial colonies. From the results of bacterial purification, 4 different bacterial isolates had different morphological characters. The results of the isolation of the colonies showed differences in a round and irregular shape, had raised and convex surfaces had red, cream, white and yellow colors, had curved and smooth edges. Tests were carried out on four bacterial isolates to determine the clear zone index. Only the L isolate in the congo red test had a diameter of clear zone 2.5 mm and with score 3 based on cellulolytic index value L isolate categorized as strong degrading cellulose bacteria.
References
Buselli, R.A.F., W.C. Otoni, and C. P. Joshi. (2007). Structure, organization, and functions of cellulose synthase complexes in higher plants. Brazilian Journal Plant Physiology 19(1): 1-13
Cappuccino, J.G., and N. Sherman. (1998). Microbiology: A Laboratory Manual. 5 th. Edition. California: Benjamin/Cummings Science Publishing. 1(5): 45-70
Choi, Y. W., I. J. Hodgkiss, and K. D. Hyde. (2005). Enzyme Production by Endophytes of Brucea Javanica. J. Agri Tech, 1(5): 55-66
Faesal, F., D.J. Nurasiah, dan Soenartiningsih. (2017). Seleksi Efektivitas Bakteri Dekomposer terhadap Limbah Tanaman Jagung. Balai Penelitian Tanaman Serealia. 1(2):106-113
Hadioetomo, R. S. (1993). Mikrobiologi Dasar Dalam Praktek : Teknik dan Prosedur Dasar Laboratorium. PT. Gramedia Pustaka Utama. Jakarta.
International Coffee Organization (ICO). (2019). Trade Statistics Data Exports. International Coffee Organization: https://www.ico.org/new_historical.asp accessed on 20 Desember 2020.
Kurniawan, A., D. Febrianti, S. P. Sari, A. A. Prihanto, E. Asriani, K. Andi, dan A. B. Sambah. (2018). Isolasi dan Identifikasi Bakteri Pedegradasi Selulosa asal Ekosistem Mangrove Tukad Sadai, Bangka Selatan. Jurnal Perikanan Pantura. 1(2): 9-16.
Lay, B. W. (1994). Analisis Mikroba di Laboratorium. PT Raja Grafindo Persada. Jakarta.
Meryandini, A., W. Widosari, B. Maranatha, T.C. Sunarti, N. Rachmania, dan H. Satria. 2009. Isolasi Bakteri Selulolitik dan Karakterisasi Enzimnya. Jurnal Sains. Institut Pertanian Bogor. 13(1):33-38.
Perez, D., dan A. Jose. (2016). Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production. Bioresource Technology.
Rooks, D. J., J.E. McDonald, J.N.I Houghton et al (2012) The microbial ecology of anaerobic cellulose degradation in municipal waste landfill. Enviroment Microbiol. 10:1310-1319.
Simanungkalit, R.D.M., D.A. Suriadikarta, R. Saraswati, D. Setyorini, dan W. Hartatik. (2006). Pupuk Organik dan Pupuk Hayati. Balai Besar Penelitian Dan Pengembangan Sumberdaya Lahan Pertanian. Bogor. Hal. 256-272.
Sinatryani, D. (2014). Kelimpahan Bakteri Selulolitik Di Muara Sungai Gunung Anyar Surabaya Dan Bancaran Bangkalan. Skripsi thesis. Universitas Airlangga.
Soeka, Y. S., dan E. Triana. (2016). Pemanfaatan limbah kulit udang untuk menghasilkan enzim kitinase dari Streptomyces macrosporeus InaCC A454. Jurnal Kimia Terapan Indonesia, 18(1):91-101.
Suardana, I.W., I.N. Suarsana, dan K.G. Wirayawan. (2007). Isolasi dan Identifikasi Bakteri Asam Laktat dari Cairan Rumen Sapi Bali sebagai Kandidat Biopreservatif. Jurnal Veteriner. 8(4) : 155-159.
Utama, C. S., Zuprizal, C. Hanim, dan Wihandoyo. (2018). Isolasi dan Identifikasi Bakteri Asam Laktat Selulolitik yang Berasal dari Jus Kubis Terfermentasi. Jurnal Aplikasi Teknologi Pangan. 7(1):1-6
