Uji Antagonistik Paenibacillus polymyxa Jav78 terhadap jamur Colletotrichum spp. secara in vitro
Abstract
Anthracnose disease in chili plants can cause yield loss. The purpose of this study was to determine the ability of Paenibacillus polymyxa Jav78 to inhibit the growth of Colletotrichum spp. and identify the antifungal compound produced by P. polymyxa Jav78. Antagonistic test of P. polymyxa Jav78 against Colletotrichum fungus was carried out in vitro. Identification of antifungal compounds using Gas Chromatography Mass Spectrometry (GCMS). The results showed that P. polymyxa Jav78 was able to inhibit the growth of the fungus Colletotrichum spp. with the percentage of inhibition ranging from 77.33% to 85.07%. The P. polymyxa Jav78 filtrate contains antifungal compounds 76.01% hexadecanoic acid, methyl ester and 16.48% imidazole-3-oxide. The results of this study provide information that P. polymyxa Jav78 can be used as a biofungicide to control anthracnose disease in chili plants.
References
Abubacker, M.N., Deepalakshmi, T. (2013). In vitro antifungal potentials of bioactive compound methyl ester of hexadecanoic acid isolated from Annona muricata Linn. (Annonaceae) Leaves. Biosci., Biotech. Res. Asia 10(2): 879-884.
Agoramoorthy, G., Chandrasekaran, M., Venkatesalu, V., Hsu, M.J. (2007). Antibacterial and antifungal activities of fatty acid methyl esters of the llind-your-eye mangrove from India. Brazilian Journal of Microbiology 38:739-742.
Banfi, E., Giuditta, S., Daniele, Z., Maria, G.M., Luciano, V., Marco, F., Maurizio, F., Maria, S.P., Sabrina, P. (2006). Antifungal and antimycobacterial activity of new imidazole and triazole derivatives, A combined experimental and computational approach. Journal of Antimicrobial Chemotherapy 58: 76–84
Beatty, P., Jensen, S.E. (2002). Paenibacillus polymyxa produces fusaricidin-type antifungal antibiotics active against Leptosphaeria maculans, the causative agent of blackleg disease of canola. Can. J. Microbiol. 48: 159–169.
Deng, Y., Zhaoxin, L., Fengxia, L., Chong, Z., Yu, W., Haizhen, Z., Xiaomei, B. (2011). Identification of LI-F type antibiotics and di-n-butyl phthalate produced by Paenibacillus polymyxa. Journal of Microbiological Methods 85: 175–182.
Helmick, R.A., Arin, E. F., Anne, M. G., Christopher, R. G., Angela, N.H., Michael, C.G., Paul, R.G. (2005). Imidazole antibiotics inhibit the nitric oxide dioxygenase function of microbial flavohemoglobin. Antimicrobial agents and chemotherapy 49(5): 1837-1843.
Hori, K., Akira, S., Michinari, K., Koichi, I., Yuri, A., Yuso, Y. (2000). Structure-activity relationships of a new antifungal imidazole, AFK-108, and related coumpounds. Chem. Pharm. Bull. 48(1): 60-64.
Human Metabolome Database. (2014). Showing metabocard for Methyl hexadecanoic acid (HMDB0061859). https://hmdb.ca/metabolites/HMDB0061859.
Khalimi, K., Darmadi, A.A.K., Suprapta, D.N. (2019). First Report on the Prevalence of Colletotrichum scovillei Associated with Anthracnose on Chili Pepper in Bali, Indonesia. Intl. J. Agric. Biol. 22: 363‒368.
Li, Y., Chen, S. (2019). Fusaricidin Produced by Paenibacillus polymyxa WLY78 Induces Systemic Resistance against Fusarium Wilt of Cucumber. Int. J. Mol. Sci. 20: 1-19.
Liu, F., Guiting, T., Xiaojuan, Z., Ying, L., Xiaofang, S., Xiaobo, Q., You, Z., Jing, X., Huabao, C., Xiaoli, C., Sirong, Z., Guoshu, G. (2016). Molecular and phenotypic characterization of Colletotrichum species associated with anthracnose disease in peppers from Sichuan Province, China. Scientific reports 6:1-17.
Pinto, M.E.A., Sthefane, G.A., Marcela, M., Nivea, P.S., Caroline, M.L., Carlos, A.R., Ezequeira, P., Susana, J., Luciana, A.R.S. (2017). Antifungal and antioxidant activity of fatty acid methyl esters from vegetable oils. Anais da Academia Brasileira de Ciencias 89(3): 1671-1681.
Prasath, K.G., Tharani, H., Mourya, S.K., Shunmugiah, K.P. (2020). Palmitic acid inhibits the virulence factors of candida tropicalis: biofilms, cell surface hydrophobicity, ergosterol biosynthesis, and enzymatic activity. Frontiers in Microbiology 11(864):1-21.
Siwach, A., Verma, P.K. (2021). Synthesis and therapeutic potential of imidazole containing compounds. BMC Chemistry 15(12): 1-69.
Than, P.P., Haryudian, P., Sitthisack, P., Paul, W.J.T., Kevin, D.H. (2008). Chilli anthracnose disease caused by Colletotrichum species. Journal of Zhejiang University Science B 9(10):764-778.
Zhai, Y., Jiu, X.Z., Tai, M.T., Jian, P.X., Ai-rong, S., Xie, B.Y., Ji, L.L., Liang, B.Z. (2021). Isolation and characterization of antagonistic Paenibacillus polymyxa HX-140 and its biocontrol potential against Fusarium wilt of cucumber seedlings. BMC Microbiology 21(75): 1-12.
Agoramoorthy, G., Chandrasekaran, M., Venkatesalu, V., Hsu, M.J. (2007). Antibacterial and antifungal activities of fatty acid methyl esters of the llind-your-eye mangrove from India. Brazilian Journal of Microbiology 38:739-742.
Banfi, E., Giuditta, S., Daniele, Z., Maria, G.M., Luciano, V., Marco, F., Maurizio, F., Maria, S.P., Sabrina, P. (2006). Antifungal and antimycobacterial activity of new imidazole and triazole derivatives, A combined experimental and computational approach. Journal of Antimicrobial Chemotherapy 58: 76–84
Beatty, P., Jensen, S.E. (2002). Paenibacillus polymyxa produces fusaricidin-type antifungal antibiotics active against Leptosphaeria maculans, the causative agent of blackleg disease of canola. Can. J. Microbiol. 48: 159–169.
Deng, Y., Zhaoxin, L., Fengxia, L., Chong, Z., Yu, W., Haizhen, Z., Xiaomei, B. (2011). Identification of LI-F type antibiotics and di-n-butyl phthalate produced by Paenibacillus polymyxa. Journal of Microbiological Methods 85: 175–182.
Helmick, R.A., Arin, E. F., Anne, M. G., Christopher, R. G., Angela, N.H., Michael, C.G., Paul, R.G. (2005). Imidazole antibiotics inhibit the nitric oxide dioxygenase function of microbial flavohemoglobin. Antimicrobial agents and chemotherapy 49(5): 1837-1843.
Hori, K., Akira, S., Michinari, K., Koichi, I., Yuri, A., Yuso, Y. (2000). Structure-activity relationships of a new antifungal imidazole, AFK-108, and related coumpounds. Chem. Pharm. Bull. 48(1): 60-64.
Human Metabolome Database. (2014). Showing metabocard for Methyl hexadecanoic acid (HMDB0061859). https://hmdb.ca/metabolites/HMDB0061859.
Khalimi, K., Darmadi, A.A.K., Suprapta, D.N. (2019). First Report on the Prevalence of Colletotrichum scovillei Associated with Anthracnose on Chili Pepper in Bali, Indonesia. Intl. J. Agric. Biol. 22: 363‒368.
Li, Y., Chen, S. (2019). Fusaricidin Produced by Paenibacillus polymyxa WLY78 Induces Systemic Resistance against Fusarium Wilt of Cucumber. Int. J. Mol. Sci. 20: 1-19.
Liu, F., Guiting, T., Xiaojuan, Z., Ying, L., Xiaofang, S., Xiaobo, Q., You, Z., Jing, X., Huabao, C., Xiaoli, C., Sirong, Z., Guoshu, G. (2016). Molecular and phenotypic characterization of Colletotrichum species associated with anthracnose disease in peppers from Sichuan Province, China. Scientific reports 6:1-17.
Pinto, M.E.A., Sthefane, G.A., Marcela, M., Nivea, P.S., Caroline, M.L., Carlos, A.R., Ezequeira, P., Susana, J., Luciana, A.R.S. (2017). Antifungal and antioxidant activity of fatty acid methyl esters from vegetable oils. Anais da Academia Brasileira de Ciencias 89(3): 1671-1681.
Prasath, K.G., Tharani, H., Mourya, S.K., Shunmugiah, K.P. (2020). Palmitic acid inhibits the virulence factors of candida tropicalis: biofilms, cell surface hydrophobicity, ergosterol biosynthesis, and enzymatic activity. Frontiers in Microbiology 11(864):1-21.
Siwach, A., Verma, P.K. (2021). Synthesis and therapeutic potential of imidazole containing compounds. BMC Chemistry 15(12): 1-69.
Than, P.P., Haryudian, P., Sitthisack, P., Paul, W.J.T., Kevin, D.H. (2008). Chilli anthracnose disease caused by Colletotrichum species. Journal of Zhejiang University Science B 9(10):764-778.
Zhai, Y., Jiu, X.Z., Tai, M.T., Jian, P.X., Ai-rong, S., Xie, B.Y., Ji, L.L., Liang, B.Z. (2021). Isolation and characterization of antagonistic Paenibacillus polymyxa HX-140 and its biocontrol potential against Fusarium wilt of cucumber seedlings. BMC Microbiology 21(75): 1-12.
Published
2023-07-19
How to Cite
PHABIOLA, Trisna Agung; KHALIMI, Khamdan.
Uji Antagonistik Paenibacillus polymyxa Jav78 terhadap jamur Colletotrichum spp. secara in vitro.
Nandur, [S.l.], v. 3, n. 3, p. 124-130, july 2023.
ISSN 2746-6957.
Available at: <https://ojs.unud.ac.id/index.php/nandur/article/view/104662>. Date accessed: 18 may 2024.
Issue
Section
Articles
