GLYCEROL TOXICITY AND QUALITY OF FROZEN-THAWED BALI CATTLE SPERMATOZOAS
Abstract
This study aimed to determine the effect of glycerol concentration on motility, viability and abnormalities of frozen thawed spermatozoa of Bali cattle. The study was conducted using a completely randomized design with 5 treatments and 6 replications. The treatments consisted of T0 (0% glycerol), T1 (6% glycerol), T2 (12% glycerol), T3 (18% glycerol), P4 (24% glycerol). The results showed that the administration of glycerol had a very significant effect (p<0.01) on the motility, viability, and abnormality of spermatozoa. Six percent glycerol in diluent able to reduce the decrease in motility, viability. and prevent the increase of spermatozoa abnormality. Toxic effects of glycerol were higher in accordance to higher concentration started in 12%.
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References
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Afriantini, R.I., S. Irnan, dan S. San. 2007. Penentuan waktu equilibrasi pada pembekuan semen kuda menggunakan bahan pengencer susu skim. Animal Production 9(3): 145- 152
Bailey, J.L., J.F. Bilodeau, and N. Cormier. 2000. Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon. J Androl. 21: 1–7
Baust, J.G., D. Gao, and J.M. Baust. 2009. Cryopreservation: an emerging paradigm change. Organogenesis. 5: 90–96.
Bearden, H.J. and J.W. Fuguay. 1980. Applied Animal Reproduction. Reston Publishing Company. Inc. Prentice Hall Company, Reston Virginia
Bogle, O.A., K. Kumar, C. Attardo-Parrinello, S.E.M. Lewis, J.M. Estanyol, and J.L. Ballescà. 2017. Identification of protein changes in human spermatozoa throughout the cryopreservation process. J. Andrology. 5(1): 10–22
Esseltine, J.L. and J.D. Scott. 2013. AKAP signaling complexes: pointing towards the next generation of therapeutic targets? Trends Pharmacol. Sci. 34: 648-655.
Evans, G. dan W.M.C. Maxwel. 1987. Salamon’s Artificial Insemination of Sheep and Goat. Sydney: Butter- worths.
Fahy, G.M. 1986. The relevance of cryoprotectant “toxicity” to cryobiology. Cryobiology. 23:1-13.
Fitriati. 2008. Kualitas spermatozoa cauda epididymis sapai peramakan ongol (PO) dalam penegencer susu, tris dan sitrat kuning telur pada penyimpanan 4-5°C. J. Anim. Prod. 10(1): 22-29.
Garcia, B.M., C. O. Ferrusola, I.M. Aparicio, A. Miro- Moran, A.M. Rodriguez, J.M.G. Bolanos, L.G. Fernandez, C.M.B.d. Silva, H. RodriguezMartinez, J.A. Tapia, and F.J. Pena. 2012. Toxicity of glycerol for the stallion spermatozoa: Effects on membrane integrity and cytoskeleton, lipid peroxidation, and mitochondrial membrane potential. Theriogeno- logy. 77(7): 1280-1289.
Glazar, A.I., S.F. Mullen, J. Liu, J.D. Benson, J.K. Critser, and E.L. Squires. 2009. Osmotic tolerance limits and membrane permeability characteristics of stallion spermatozoa treated with cholesterol. Cryobiology. 59: 201-206.
Hammerstedt, R.H., J.K. Graham, and J.P. Nolan. 1990. Cryopreservation of mammalian sperm: what we ask them to survive. J. Androl. 11: 73–88
Hugana, T., Y.T. Grohn, E. Kommisrud, E. Rosptad, and O. Raksen. 2007. Effects of sperm concentration at semen collection and storage period of frozen semen on dairy cow conception. Anim. Reprod. Sci. 97: 1-11.
Holt, W.V. and R.D. North. 1991. Cryopreservation, actin localization and thermotropic phase transitions in ram spermatozoa. J. Reprod. Fert. 91: 451-461.
Johnston, S.D., N. Satake, Y. Zee, C. LópezFernández, W.V. Holt, and J. Gosálvez. 2012. Osmotic stress and cryoinjury of koala sperm: an integrative study of the plasma membrane, chromatin stability and mitochondrial function. Reprod 143(6): 787–797.
Kopeika, J., A. Thornhill, and Y. Khalaf. 2015. The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence. Hum Reprod Update. 21(2): 209–227.
Koppers, A.J., M.L. Garg, and R.J. Aitken. 2010. Sti- mulation of mitochondrial reactive oxygen species production by unesterified, unsaturated fatty acids in defective human spermatozoa. Free Radic Biol Med. 48: 112-119.
McCarthy, M.J., J. Baumber, P.H. Kass, and S.A. Meyers. 2009. Osmotic stress induces oxidative cell dama- ge to rhesus macaque spermatozoa1. Biol Reprod. 82(3): 644– 651.
Kumar, S., K.L. Sahni, and G. Moohan. 1992. Effect of different levels of glycerols and yolk on freezing and storage of buffalo semen in milk, tris, and sodium citrate buffers. Buffalo J. 2: 151-156.
Miller, J.S. and D.G. Cornwell. 1975. The role of cryoprotective agents as hydroxyl radical scavengers. Cryobiology. 5: 585-588.
Ortega, F.C, L.G. Fernandez, J.M. Morrell, C.S. Sandoval, B.M. Garcia, and H. Rodriguez-Martinez. 2009. Li- pid peroxidation, assessed with BODIPY-C11, increases after cryopreservation of stallion spermatozoa, is stallion-dependent and is related to apoptotic-like changes. Reproduction. 138: 55-63.
Parera, F., Z. Prihatini, D.F. Souhoka, and M. Rizal. 2009. Pemanfaatan sari wortel sebagai pengenceralterna- tif spermatozoa epididimis sapi Bali. J. Indon. Trop. Anim. Agric. 34(1): 50-56.
Parrish, J. 2003. Techniques in Domestic Animal Reproduction Evaluation and Freezing of Semen. http:// www.wisc.edu/anscirepro/
Pena, F.J., H. Rodriguez-Martinez, J.A. Tapia, C. Or-tega-Ferrusola, L. Gonzalez-Fernandez, and B. Macias-Garcia. 2009. Mitochondria in mammalian sperm physiology and pathology: a review. Reprod Domest Anim. 44: 345-349.
Robbins, R.K., R.G. Saacke, and P.T. Chandler. 1976. Influence of freeze rate, thaw rate, and glycerol level on acrosomal retention and survival of bovine spermatozoa frozen in French straws. J. Anim. Sci. 42: 145-154.
Rosadi, B., T. Sumarsono, dan Darmawan. 2015. Motili- tas permatozoa kerbau umpur pada penyimpanan semen beku dalam es. JIIP. 18(2): 98-101.
Rizal, M. dan Herdis. 2010. Peranan Antioksidan Dalam Meningkatkan Kualitas Semen Beku. Wartazoa. 20(3): 139-145.
Scherle, C.C., M.S. Maia, S. Bicudo, L. Rodello, and H. Azevedo. 2011. Lipid peroxidation and generation of hydrogen peroxide in frozen-thawed ram semen supplemented with catalase or Trolox. Small Rum Res. 95(2–3): 144–149.
Setiono, N., S. Sri, dan E.S. Purnama. 2015. Kualitas Se- men Beku Sapi Brahman dengan Dosis Krioprotek- tan Gliserol yang Berbeda dalam Bahan Pengencer Tris Sitrat Kuning Telur. Jurnal Ilmiah Peternakan Terpadu. 3(2): 61-69.
Sinurat, L.H., S. Erina, dan B. Rosadi. 2020. Efek penyimpanan epididimis sapi Bali pada suhu 5°C terhadap kualitas spermatozoa. Jurnal Peternakan Sriwijaya. 9(2): 27-34.
Siswanto. 2006. Kualitas Semen di dalam Pengencer Tris dan Natrium Sitrat dengan Berbagai Sumber Karbohidrat dan Level Gliserol Pada Proses Kriop- reservasi Semen Rusa Timor (Cervus timorensis). Tesis. Sekolah Pascasarjana Institut Pertanian Bogor. Bogor.
Sinha, S., B.C. Deka, M.K. Tamulu, and B.N. Borgohain. 1992. Effect of equilibration period and glycerol level in tris extender of quality of frozen goat semen. Indian Vet. J. 69: 1107-1110.
Supriatna, I. dan F.H. Pasaribu. 1992. In Vitro Fertilisasi, Transfer Embrio dan Pembekuan Embrio. Pusat Antar Universitas, Institut Pertanian Bogor, Bogor.
Susilawati, T. 2000. Teknologi Preservasi dan Krioprevasi Spermatozoa dan Ova. Tesis. Program Pasca Sarjana Fakultas Peternakan Universitas Brawijaya, Malang.
Ugur, M.R., A.S. Abdelrahman, H.C. Evans, A.A. Gilmore, M. Hitit, R.I. Arifiantini, B. Purwantara, A. Kaya, and E. Memili. 2019. Advances in cryopreservation of bull sperm. Front. Vet. Sci. 6: 1-15.
Watson, P.F. 2000. The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci. 60-61: 481-92.
Afriantini, R.I., S. Irnan, dan S. San. 2007. Penentuan waktu equilibrasi pada pembekuan semen kuda menggunakan bahan pengencer susu skim. Animal Production 9(3): 145- 152
Bailey, J.L., J.F. Bilodeau, and N. Cormier. 2000. Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon. J Androl. 21: 1–7
Baust, J.G., D. Gao, and J.M. Baust. 2009. Cryopreservation: an emerging paradigm change. Organogenesis. 5: 90–96.
Bearden, H.J. and J.W. Fuguay. 1980. Applied Animal Reproduction. Reston Publishing Company. Inc. Prentice Hall Company, Reston Virginia
Bogle, O.A., K. Kumar, C. Attardo-Parrinello, S.E.M. Lewis, J.M. Estanyol, and J.L. Ballescà. 2017. Identification of protein changes in human spermatozoa throughout the cryopreservation process. J. Andrology. 5(1): 10–22
Esseltine, J.L. and J.D. Scott. 2013. AKAP signaling complexes: pointing towards the next generation of therapeutic targets? Trends Pharmacol. Sci. 34: 648-655.
Evans, G. dan W.M.C. Maxwel. 1987. Salamon’s Artificial Insemination of Sheep and Goat. Sydney: Butter- worths.
Fahy, G.M. 1986. The relevance of cryoprotectant “toxicity” to cryobiology. Cryobiology. 23:1-13.
Fitriati. 2008. Kualitas spermatozoa cauda epididymis sapai peramakan ongol (PO) dalam penegencer susu, tris dan sitrat kuning telur pada penyimpanan 4-5°C. J. Anim. Prod. 10(1): 22-29.
Garcia, B.M., C. O. Ferrusola, I.M. Aparicio, A. Miro- Moran, A.M. Rodriguez, J.M.G. Bolanos, L.G. Fernandez, C.M.B.d. Silva, H. RodriguezMartinez, J.A. Tapia, and F.J. Pena. 2012. Toxicity of glycerol for the stallion spermatozoa: Effects on membrane integrity and cytoskeleton, lipid peroxidation, and mitochondrial membrane potential. Theriogeno- logy. 77(7): 1280-1289.
Glazar, A.I., S.F. Mullen, J. Liu, J.D. Benson, J.K. Critser, and E.L. Squires. 2009. Osmotic tolerance limits and membrane permeability characteristics of stallion spermatozoa treated with cholesterol. Cryobiology. 59: 201-206.
Hammerstedt, R.H., J.K. Graham, and J.P. Nolan. 1990. Cryopreservation of mammalian sperm: what we ask them to survive. J. Androl. 11: 73–88
Hugana, T., Y.T. Grohn, E. Kommisrud, E. Rosptad, and O. Raksen. 2007. Effects of sperm concentration at semen collection and storage period of frozen semen on dairy cow conception. Anim. Reprod. Sci. 97: 1-11.
Holt, W.V. and R.D. North. 1991. Cryopreservation, actin localization and thermotropic phase transitions in ram spermatozoa. J. Reprod. Fert. 91: 451-461.
Johnston, S.D., N. Satake, Y. Zee, C. LópezFernández, W.V. Holt, and J. Gosálvez. 2012. Osmotic stress and cryoinjury of koala sperm: an integrative study of the plasma membrane, chromatin stability and mitochondrial function. Reprod 143(6): 787–797.
Kopeika, J., A. Thornhill, and Y. Khalaf. 2015. The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence. Hum Reprod Update. 21(2): 209–227.
Koppers, A.J., M.L. Garg, and R.J. Aitken. 2010. Sti- mulation of mitochondrial reactive oxygen species production by unesterified, unsaturated fatty acids in defective human spermatozoa. Free Radic Biol Med. 48: 112-119.
McCarthy, M.J., J. Baumber, P.H. Kass, and S.A. Meyers. 2009. Osmotic stress induces oxidative cell dama- ge to rhesus macaque spermatozoa1. Biol Reprod. 82(3): 644– 651.
Kumar, S., K.L. Sahni, and G. Moohan. 1992. Effect of different levels of glycerols and yolk on freezing and storage of buffalo semen in milk, tris, and sodium citrate buffers. Buffalo J. 2: 151-156.
Miller, J.S. and D.G. Cornwell. 1975. The role of cryoprotective agents as hydroxyl radical scavengers. Cryobiology. 5: 585-588.
Ortega, F.C, L.G. Fernandez, J.M. Morrell, C.S. Sandoval, B.M. Garcia, and H. Rodriguez-Martinez. 2009. Li- pid peroxidation, assessed with BODIPY-C11, increases after cryopreservation of stallion spermatozoa, is stallion-dependent and is related to apoptotic-like changes. Reproduction. 138: 55-63.
Parera, F., Z. Prihatini, D.F. Souhoka, and M. Rizal. 2009. Pemanfaatan sari wortel sebagai pengenceralterna- tif spermatozoa epididimis sapi Bali. J. Indon. Trop. Anim. Agric. 34(1): 50-56.
Parrish, J. 2003. Techniques in Domestic Animal Reproduction Evaluation and Freezing of Semen. http:// www.wisc.edu/anscirepro/
Pena, F.J., H. Rodriguez-Martinez, J.A. Tapia, C. Or-tega-Ferrusola, L. Gonzalez-Fernandez, and B. Macias-Garcia. 2009. Mitochondria in mammalian sperm physiology and pathology: a review. Reprod Domest Anim. 44: 345-349.
Robbins, R.K., R.G. Saacke, and P.T. Chandler. 1976. Influence of freeze rate, thaw rate, and glycerol level on acrosomal retention and survival of bovine spermatozoa frozen in French straws. J. Anim. Sci. 42: 145-154.
Rosadi, B., T. Sumarsono, dan Darmawan. 2015. Motili- tas permatozoa kerbau umpur pada penyimpanan semen beku dalam es. JIIP. 18(2): 98-101.
Rizal, M. dan Herdis. 2010. Peranan Antioksidan Dalam Meningkatkan Kualitas Semen Beku. Wartazoa. 20(3): 139-145.
Scherle, C.C., M.S. Maia, S. Bicudo, L. Rodello, and H. Azevedo. 2011. Lipid peroxidation and generation of hydrogen peroxide in frozen-thawed ram semen supplemented with catalase or Trolox. Small Rum Res. 95(2–3): 144–149.
Setiono, N., S. Sri, dan E.S. Purnama. 2015. Kualitas Se- men Beku Sapi Brahman dengan Dosis Krioprotek- tan Gliserol yang Berbeda dalam Bahan Pengencer Tris Sitrat Kuning Telur. Jurnal Ilmiah Peternakan Terpadu. 3(2): 61-69.
Sinurat, L.H., S. Erina, dan B. Rosadi. 2020. Efek penyimpanan epididimis sapi Bali pada suhu 5°C terhadap kualitas spermatozoa. Jurnal Peternakan Sriwijaya. 9(2): 27-34.
Siswanto. 2006. Kualitas Semen di dalam Pengencer Tris dan Natrium Sitrat dengan Berbagai Sumber Karbohidrat dan Level Gliserol Pada Proses Kriop- reservasi Semen Rusa Timor (Cervus timorensis). Tesis. Sekolah Pascasarjana Institut Pertanian Bogor. Bogor.
Sinha, S., B.C. Deka, M.K. Tamulu, and B.N. Borgohain. 1992. Effect of equilibration period and glycerol level in tris extender of quality of frozen goat semen. Indian Vet. J. 69: 1107-1110.
Supriatna, I. dan F.H. Pasaribu. 1992. In Vitro Fertilisasi, Transfer Embrio dan Pembekuan Embrio. Pusat Antar Universitas, Institut Pertanian Bogor, Bogor.
Susilawati, T. 2000. Teknologi Preservasi dan Krioprevasi Spermatozoa dan Ova. Tesis. Program Pasca Sarjana Fakultas Peternakan Universitas Brawijaya, Malang.
Ugur, M.R., A.S. Abdelrahman, H.C. Evans, A.A. Gilmore, M. Hitit, R.I. Arifiantini, B. Purwantara, A. Kaya, and E. Memili. 2019. Advances in cryopreservation of bull sperm. Front. Vet. Sci. 6: 1-15.
Watson, P.F. 2000. The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci. 60-61: 481-92.
Published
2022-11-24
How to Cite
S. A., LESTARI; ., DARMAWAN; ROSADI, B..
GLYCEROL TOXICITY AND QUALITY OF FROZEN-THAWED BALI CATTLE SPERMATOZOAS.
Majalah Ilmiah Peternakan, [S.l.], v. 25, n. 2, p. 79-84, nov. 2022.
ISSN 2656-8373.
Available at: <https://ojs.unud.ac.id/index.php/mip/article/view/97177>. Date accessed: 27 apr. 2024.
doi: https://doi.org/10.24843/MIP.2022.V25.i02.p04.
Section
Articles
