EVALUATION AND SELECTION OF ALFALFA MUTANT RESULTS OF GAMMA RAY IRRADIATION ON ABIOTIC STRESS
Abstract
Alfalfa is a leguminous protein source with high nutritional value and economic value. Abiotic stress inhibits growth and reduces alfalfa production in many aquaculture systems. New varieties were developed by gamma-ray mutation induction. The selection was carried out to obtain superior abiotic stress-tolerant plants. This study aims to evaluate and select alfalfa mutants under abiotic stress conditions. The design used in this study was a comple- tely randomized design (CRD) with a factorial pattern. The first factor is the type of acid-tolerant mutants, namely A44, A54, A35, and A45. The second factor is the PEG level which is 10%, 15%, and 20%. The variables observed included viability, plant height, number of leaves, leaf wilting, leaf color, media shrinkage, and wet weight. Alfalfa mutants resulting from acid-tolerant gamma irradiation were evaluated under dry stress conditions. The results showed that mutation induction resulted in various responses to growth. Gamma-ray irradiation produces plants that are tolerant to abiotic stress conditions. From the selection results obtained superior mutant candidates that are tolerant of acid stress and dry conditions. The A44 mutant had better tolerance to dry stress than others and became an acid- and dry-tolerant alfalfa mutant.
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Due, M.S., A. Yunus, dan A. SusilowatI. 2019. Keragam- an pisang (Musa spp.) hasil iradiasi sinar gamma secara in vitro berdasarkan penanda morfologi. PROS SEM NAS MASY BIODIV INDON Volume 5, Nomor 2. ISSN: 2407-8050 Halaman: 347-352. DOI: 10.13057/psnmbi/m050236.
Dwiyani, R. 2015. Kultur Jaringan Tanaman. Pelawa Sari Denpasar Barat.
Havidzati, N., P.D.M.H. Karti, dan I. Prihantoro. 2017. Morphology Response of Alfalfa (Medicago sativa L.) based on Level Gamma Ray Irradiation with Tissue Culture Methods. Proceedings Book, 52.
Hemon, F. 2009. Induksi Mutasi Dengan Iradiasi Sinar Gamma dan Seleksi In Vitro Untuk Mendapatkan Embrio Somatik Kacang Tanah Yang Toleran Po- lietilena Glikol. Jurnal Agrotropika 14(2): 67 – 72.
Khaerana, K., M. Ghulamahdi, dan E.D. Purwakusumah. 2008. Pengaruh cekaman kekeringan dan umur panen terhadap pertumbuhan dan kandungan xant- horrhizol temulawak (Curcuma xanthorrhiza roxb.). Indonesian Journal of Agronomy, 36(3), 8146.
Kadir, A., S.H. Sutjahjo, G.A. Wattimena, dan I. Mariska. 2007. Pengaruh iradiasi sinar Gamma pada pertum- buhan kalus dan keragaman planlet tanaman nilam.
Kumar, S. 2011. Biotechnological advancements in alfalfa improvement. Journal of applied genetics, 52(2), 111-124.
Latefi, A.A.H.A, and P. Ahmad. 2015. Legumes and bre- eding under abiotic stress: An overview. In: Azooz MM, Ahmad P (eds).Legumes under Environmental Stress: Yield, Improvement and Adaptations. First Edition. John Wiley & Sons, Ltd.
Liu, H., X. Wang, D. Wang, Z. Zou, and Z. Liang. 2011. Effect of drought stress on growth and accumulation of active constituents in Salvia miltiorrhiza Bunge.Industrial Crops and Products, 33(1), 84-88.
Liu, X., Y. Fan, J. Long, R. Wei, R. Kjelgren, C. Gong, and J. Zhao. 2013. Effects of soil water and nitrogen availability on photosynthesis and water use effici- ency of Robinia pseudoacacia seedlings. Journal of
Environmental Sciences, 25(3), 585-595. Manpaki, S.J., P.D.M. Karti, dan I. Prihatoro. 2017. Respon pertumbuhan eksplan tanaman Lamtoro (Leucaena leucocephala cv. Tarramba) terhadap cekaman kemasaman media dengan level pemberi- an aluminium melalui kultur jaringan. Jurnal Sain
Peternakan Indonesia, 12(1), 71-82.
Moore, J.P., M. Vicré‐Gibouin, J.M. Farrant, and A. Dri-
ouich. 2008. Adaptations of higher plant cell walls to water loss: drought vs desiccation. Physiologia plantarum, 134(2), 237-245.
Mulyani, A., dan M. Sarwani. 2013. Karakteristik dan potensi lahan sub optimal untuk pengembangan pertanian di Indonesia.
Muthukumar, T., P. Priyadharsini, E. Uma, S. Jaison, and R.R. Pandey. 2014. Role of arbuscular mycorrhizal fungi in alleviation of acidity stress on plant growth. In: Miransari M (ed.), Use of Microbes for the Alle- viation of Soil Stresses. Springer Science+Business Media, New York, pp. 43–71.
Nur, A., K. Syaruhddin, dan Herawati. 2015. Pengaruh Radiosensivisitas Iradiasi Sinar Gamma Terhadap Perkembangan Kecambah dan Pertumbuhan Vege- tatif Tanaman M1 Sorgum Manis (Sorghum Bicolor L.). Prosiding Seminar Nasional Serealia.
Qosim, W.A., R. Purwanto, dan G.A. Wattimena. 2007. Pengaruh Iradiasi Sinar Gamma terhadap Kapasitas Regenerasi Kalus Nodular Tanaman Manggis. Ha- yati Journal of Biosciences, 14(4), 140-144.
Radović, J., D. Sokolović, and J.J.B.A.H. Marković. 2009. Alfalfa-most important perennial forage legume in animal husbandry. Biotechnology in Animal Hus- bandry, 25(5-6-1), 465-475.
Sajimin. 2011. Medicago sativa L (alfalfa) sebagai tanam- an pakan ternak harapan di Indonesia. J. Wartazoa 2(21): 91-98.
Savitri, E.S. 2010. Pengujian in vitro beberapa varietas kedelai (Glycine max L. merr) toleran kekeringan menggunakan polyethylene glikol (PEG) 6000 pada media padat dan cair. El-Hayah: Jurnal Biologi, 1(2).
Sikuku, P.A., G.W. Netondo, J.C. Onyango, and D.M. Musyimi. 2010. Effects of water deficit on physi- ology and morphology of three varieties of nerica rainfed rice (Oryza sativa L.).
Soeranto, H. 2003. Peran iptek nuklir dalam pemuliaan tanaman untuk mendukung industri pertanian. Puslitbang Teknologi Isotop dan Radiasi, Badan Tenaga Nuklir Nasional (BATAN).
Sopandie, D. 2013. Fisiologi adaptasi tanaman terhadao cekaman abiotik pada agroekosistem tropika. PT Penerbit IPB Press.
Subantoro, R. 2009. Mengenal Karakter Mutan Alfalfa (Medicago sativa L.). MEDIAGRO. Vol 5 (2): HAL 50 – 62.
Sutjahjo, S.H., K. Abdul dan M. Ika. 2007. Effectiveness Of Polyethilene Glycol As A Selective Agent On Gamma Irradiated Patchouli Calii For Tolerance To Drought Stress. JIPI, 9(1), 48-57.
Toker, C., H. Canci, and T. Yildirim. 2007. Evaluation of perennial wild Cicer species for drought resistance. Genetic resources and crop evolution, 54(8), 1781- 1786.
Yusnita, Y. 2015. Kultur Jaringan Tanaman Sebagai Teknik Penting Bioteknologi Untuk Menunjang Pembangunan Pertanian. Aura Publishing Bandar Lampung.
Zulkarnain, Z. 2009. Kultur Jaringan Tanaman: Solusi perbanyakan tanaman budi daya. Bumi Aksara.
Published
2022-02-28
How to Cite
D.N., HARIANJA; KARTI, P.D.M.H.; PRIHANTORO, I..
EVALUATION AND SELECTION OF ALFALFA MUTANT RESULTS OF GAMMA RAY IRRADIATION ON ABIOTIC STRESS.
Majalah Ilmiah Peternakan, [S.l.], v. 25, n. 1, p. 46-51, feb. 2022.
ISSN 2656-8373.
Available at: <https://ojs.unud.ac.id/index.php/mip/article/view/87221>. Date accessed: 25 apr. 2024.
doi: https://doi.org/10.24843/MIP.2022.v25.i01.p09.
Section
Articles
