THE LENGTH GONAD AND NUMBER OF PRIMORDIAL GERM CELL OF WHITE LEGHORN CHICKEN

Abstrak

Primordial germ cells (PGCs) in poultry has significant potential for use in cell-based studies and conservation of poultry germplasm. The purpose of this study was to determine whether the length and position of the gonad affect the number of PGCs. The fertilized egg of White Leghorn chicken was incubated for 6 days and for observing variables of length and number of PGC-gonad on the right and left side used 27 embryo samples. Tweezer is used to clean the stomach and its content until visible mesonephros. The gonad attached to the mesonephros on the right and left, then the length of the gonad was measured. To remove the gonad from the mesonephros, a tweezer is used and is carried out carefully until it is released and the gonad are not broken. Next, the PGC-gonad isolation was performed to determine the number of cell using a hemocytometer, then seen and counted under a microscope. The observation showed that the length of the right and left gonad length showed no difference (191.56 vs 201.96 ?m) (P>0.05). Meanwhile, the number of PGCs is influenced by the position of the gonad (82.20 vs 122.40 cells) (P<0.05). It can be concluded, the left side gonad has a longer size and the number of PGCs is more than the right side gonad.