IN VITRO: ANTHELMINTIC ACTIVITY OF AQUEOUS LEAF EXTRACT OF Indigofera tintoria L AGAINTS Haemonchus contortus
Abstract
Gastrointestinal nematodes are one of the causes of reduced production efficiency in sheep and goats in Indone- sia, especially the Haemonchus contortus abomasum parasite. This parasite causes Haemonchosis disease which causes anemia and is fatal for productive sheep and goats. This study evaluated the inhibition of egg hatchability and mortality of adult H. contortus worms from in vitro infusaion of Indigofera tintoria L leaves. Indigofera leaf infusaion testing at five concentrations of 20%, 40%, 60% and 80%(w/v) was used both treatments. Albendazole at a dose 0f 2 mg/ml was used as apositive control, while negative control used 0.9% NaCl solution. At the highest concentration of 80% Indigofera leaf infusaion was able to inhibit egg hatching by 95.60% and had a mortality rate against adult H. contortus worms by 89.67%. The 80% concentration has the same effect when compared to the positive control using Albendazole. Phytochemical analysis of Indigofera tintoria L leaves contains condensed tannins and is useful as a bioanthelmintic.
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References
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Sani, R. A., G. D. Gray, and R. L. Baker. 2004. Worm Control for Small Ruminants in Tropical Asia. ACIAR Monograph Series 113. Australian Centre for International Agriculture Research.
Setyono, W. 2014. Kontrol Parasit Menggunakan Hijauan sebagai Agen Anthelmintika pada Kambing Bligon Betina. Tesis. Fakultas Peternakan. Universitas Gadjah Mada. Yogyakarta.
Setyono, W., Kustantinah, E. Indarto, N. D. Dono, Zup- rizal, and I. H. Zulfa. 2019. Calliandra calothyrsus and Artocarpus heterophyllus as anti parasite for Bligon Goat. J. Indonesian Trop. Anim. Agric. 44(4): 400-407.
Alonzo-Diaz, M. A., J. F. J. Torres-Acosta, C. A. Sando- val-Castro, and H. Hoste. 2011. Tannins in tropical tree fodders fed to small ruminants: A friendly foe. Small Rumin. Res. 89: 164-173.
Barrau, E., N. Fabre, I. Fouraste, and H. Hoste. 2005. Effect of bioactive compounds from sainfoin (Onob- rychis viciifolia Scop.) on the in vitro larval migra- tion of Haemonchus contortus: Role of tannins and flavonol glycosides. Parasitology 131: 531–538.
Coles, G. C., C. Bauer, F. H. M. Borgsteede, S. Geerts, T. R. Klei, and M. A. Taylor. 2000. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of ant- helmintic resistance in nematodes of veterinary importance. Vet. Parasitol. 44: 35– 44
Eguale, T., D. Tadesse, and M. Giday. 2011. In vitro ant- helmintic activity of crude extracts of five medicinal plants against egg-hatching and larval development of Haemonchus contortus. J. Ethnopharmacol. 98: 319-324.
Ferreia, L. E., P. M. N. Castro, A. C. S. Changas, S. C. Franca, and R. O. Beleboni. 2013. In vitro ant- helmintic activity of aqueous leaf extract of Annona muricata L (Annonaceae) against Haemonchus contortus from sheep. Experimental Parasitology. 134: 327-332
Gilleard, J. S. 2006. Understanding anthelmintic resis- tance: The need for genomics and genetics. Int. J. Parasitol. 36: 1227-1239
Githiori, J. B. 2004. Evaluation of anthelmintic proper- ties of ethnoveterinary plants preparation used as
livestock dewormers by pastoralist and small holder farmers in Kenya. Doctoral Disertation. Departemen Biomedical Sciences and Veterinary Public Health, SLU. Acta Universitatis Agriculture Science. 76.
Haryuningtyas, D. and W. T. Artama. 2008. Analisis se- kuen Gen Tubulin-β Isotipe 1 Cacing Haemonchus contortus isolat resisten terhadap benzimidazole pada domba di Indonesia. J. AgroBiogen. 4: 45–50
Hoste, H., F. Jackson, S. Athanasiadou, S. M. Thamsborg, and S. O. Hoskin. 2006. The effects of tannin-rich plants on parasitic nematodes in ruminants. Trends Parasitol. 22: 253–261
Jackson, F., M. Varady, and D. J. Bartley. 2012. Mana- ging anthelmintic resistance in goats-can we learn lessons from sheep? Small Rumin. Res. 103: 3–9.
Kaplan, R. M. 2004. Drug resistance in nematodes of veterinary importance: A status report. TRENDS Parasitol. 20: 478–481.
Kustantinah, A., J. Daryatmo, E. R. Orskov, R.W. Mayes, and H. Hartadi. 2010. Utilization of Cassava leaf and Carrica papaya leaf as feed and anthelmintic for goat. Proceedings of the British Society of Animal Science and the Agricultural Research Forum. 1: 114.
Martins, A. C., P. L. F. Bergamasco, G. Felippelli, J. H. Tebaldi, M. F. D. Moraes, A. J. P. Testi, I. M. Lapera, and E. G. L. Hoppe. 2017. Haemonchus contortus resistence to monepantel in sheep: fecal egg count reduction and metabolism of methionine, cystine, and inorganic sulfur in sheep. Br. J. Nutr. 70: 647- 661
Mengist, Z., N. Abeb, G. Gugsa, and N. Kumar. 2014. Assessment of small ruminant Haemonchus and its associated risk factors in and around Finoteselam, Ethiopia. Journal of Agriculture and Veterinary Science. 7(12): 36-41.
Miller, J. E. and D. W. Haorohov. 2006. Immunilogical aspects of nematode parasite control in sheep. Jo- urnal of Animal Science. 84.E124-E132.
Nawaz, M., S. M. Sajid, J. Zubair, Z. Hussain, Abbasi, A. Mohi-Ud-Din, and M. Waqas. 2014. In vitro and in vivo anthelmintic activity of leaves Azadirachta indica, Dalbergia sisso and Morus alba againts Haemonchus contortus. Global Veterinaria. 13: 996-1001.
Ngeh, J. T., W. Jacob, N. Mopoi, and D. Sali. 2007. Ethno veterinary medicine. A practical approach to the treatment of cattle diseases in Sub-Saharan Afri- can. 2nd Edition. Agromisa Foundation and CTA, Wageningen. 88.
Oliveira, L. M. B., C. M. L. Bevilaqua, I. T. F. Macedo, S. M. Morais, M. V. B. Monteiro, C. C. Campello, W. L. C. Ribeiro, and E. F. K. Batista. 2011. Effect of six tropical tanniferous plant extracts on larval exsheathment of Haemonchus contortus. Rev. Bras. Parasitol. Vet. 20: 155–160
Sakti, A. A., R. W. Kustantinah, R. W. Nurcahyo, L.
Perdani, and M. Ekaningrum. 2018. Extraction of condensed tannins from tropical plants as affected by leaves maturity, maceration time, and centrifugal force. Materials Science Forum. 78 – 84
Sani, R. A., G. D. Gray, and R. L. Baker. 2004. Worm Control for Small Ruminants in Tropical Asia. ACIAR Monograph Series 113. Australian Centre for International Agriculture Research.
Setyono, W. 2014. Kontrol Parasit Menggunakan Hijauan sebagai Agen Anthelmintika pada Kambing Bligon Betina. Tesis. Fakultas Peternakan. Universitas Gadjah Mada. Yogyakarta.
Setyono, W., Kustantinah, E. Indarto, N. D. Dono, Zup- rizal, and I. H. Zulfa. 2019. Calliandra calothyrsus and Artocarpus heterophyllus as anti parasite for Bligon Goat. J. Indonesian Trop. Anim. Agric. 44(4): 400-407.
Published
2024-04-10
How to Cite
W., SETYONO et al.
IN VITRO: ANTHELMINTIC ACTIVITY OF AQUEOUS LEAF EXTRACT OF Indigofera tintoria L AGAINTS Haemonchus contortus.
Majalah Ilmiah Peternakan, [S.l.], v. 27, n. 2, p. 105-109, apr. 2024.
ISSN 2656-8373.
Available at: <https://ojs.unud.ac.id/index.php/mip/article/view/120931>. Date accessed: 04 feb. 2025.
doi: https://doi.org/10.24843/MIP.2024.V27.i02.p08.
Section
Articles
