INVESTIGASI BAHAN AKTIF PEGAGAN BERDASARKAN JEJARING DENGAN PROTEIN TARGET : STUDI PENCARIAN OBAT ALZHEIMER SECARA IN SILICO

Abstrak

ABSTRACT

Alzheimer’s disease is a top-10 deadly neurodegenerative disease based on WHO in 2019. Its characterized by a reduction in Choline Acetyltransferase (ChAt) of a substance that acts in the production of asetikolin. One of the ingredients of nature known to play a part in the increased memory of pegagan. To learn about the line of active compound mechanisms used in the treatment of alzheimer's, the study uses an in silico approach by analyzing target proteins and the compound's active compound on a network of compounds. This study used 12 test compounds and the ChAt receptor molecules. Networking design the interactions of these compounds using the sea target and STRING databases. The results of that interactions are visualized on the cytoscape device. Furthermore, the results also suggested thateight of compounds’stest that have interactions with ChAt receptors whereas the target proteins directly linked to ChAt receptors namely Tryptophan 5-hydroxylase 1 (PDB ID: 5tpg)  and Zinc finger protein GLI (PDB ID: 4kmd). Thus, the pegagan active compound that acts most closely on protein target is Asiatic Acid, Brahmic Acid, -Humulene, -Caryophyllene, Bicyclogermacrene, Germacrene B, -Pinene, Caryophyllene.

Keywords: Alzheimer's disease, Choline Acetyltransferase (ChAt), pegagan, in silico, network interaction