Neuroprotective Effect Of Papaya (Carica papaya L.) Leaf Extract In Pyramidal Cells Of Hippocampus And Cerebral Cortex In Trimethyltin-Induced Mice (Mus musculus L.)

Abstract

This study aimed to determine the histological features of the hippocampus and cerebral cortex of mice (Mus musculus L.) that induced by TMT which is given papaya leaf extract (Carica papaya L.) and to determine the optimum dose of neuroprotective papaya leaf extract.The study used a Completely Randomized Design (CRD) with five treatments consisting of K+ (only TMT-induced at a dose of 0,6 mg/kgBW), K- (only given Na-CMC 0.5%), P1 (TMT-induced and given papaya leaf extract dose of 500 mg / kgBW) , P2 (TMT-induced and given papaya leaf extract at a dose of 1000 mg/kgBW), P3 (TMT-induced and given papaya leaf extract at a dose of 2000 mg/kgBW). The parameters observed were pyramidal cell necrosis and the percentage of pyramidal cells damage in the hippocampus and cerebral cortex. The results showed a decrease in the percentage of pyramidal cell damage in the hippocampus at P1 by 26,46%, P2 by 28,91%, and P3 by 26,67%. The percentage of pyramidal cell damage in the cerebral cortex decreased in P1 by 9,62%, P2 by 11,14%, and P3 by 14,88%. The optimum dose of papaya leaf extract which is neuroprotective is 2000 mg/kgBW (P3).

 Keywords: papaya leaf, TMT, neuroprotective, necrosis, cerebrum, hippocampus