Optimization Of Isolation Method Of Carrageenan From Kappaphycuss Alvarezii Doty Using Factorial Experimental Design
Abstract
Kappaphycus alvarezii Doty is a species of red algae ( Rhodophyceae) producing kappa carrageenan. The value of carrageenan
will increase if it is able to form thermoreversible gels. The quality of carrageenan is determined by the isolation method which
includes several stages, namely soaking, extraction, purification, and drying. This study aims to obtain an optimum isolation
method of carrageenan from seaweed Kappaphycus alvarezii Doty using the factorial experimental design software, Design
Expert Version.7.0.0. with the characteristics that meet the standards set by FAO.
The seaweed was collected from the sea in Nusa Lembongan, Bali Province. The carrageenan isolation method was optimized by
varying three factors, namely the concentration of NaOH (4% and 8%), extraction time (0.5 hour and 3 hours), and precipitation
time (0.25 hour and 0.5 hour) in order to obtain 8 formulae which then went through an evaluation of the physical and chemical
characteristics of the carrageenan. The physical and chemical characteristics include the yield, viscosity, sulfate content, and ash
content, analyzed using Design Expert Version 7.0.0 program to determine the optimum method of isolation.
The analysis showed that the optimum method of carrageenan isolation was using NaOH with a concentration of 4.40%, the
extraction time of 2.16 hours and the precipitation time of 0.29 hour. The physical and chemical characteristics of carrageenan
with the optimum isolation method include the yield of 25.73%, the viscosity of 11.92 cPs, sulfate content of 31.95% and the ash
content of 26.16% which have met the standards set by FAO.
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References
Methods. London: Cambridge University Press.
[2] Rowe, R.C., P. J. Sheskey, and J. W. Paul. 2009. Handbook of
Pharmaceutical Exipients. Sixth Edition. London: Pharmaceutical
Press.
[3] Desiana, E., and T. Y. Hendrawati. 2015. Seminar Nasional Sains
dan Teknologi: Pembuatan Karagenan dari Eucheuma cottonii
dengn Ekstraksi KOH Mengggunakan Variabel Waktu Ekstraksi.
ISSN: 2407-1846.
[4] Anggadiredja, J., Z. Achmad., P. Heri, and S. Istani. 2006. Rumput
Laut: Pembudidayaan, Pengolahan dan Pemasaran Komoditas
Perikanan Potensial. Jakarta: Penebar Swadaya.
[5] Distantina, S., Fadilah, Y. C. Danarto, Wiratni, and M. Fahrurrozi.
2009. Efek Bahan Kimia pada Tahap Presipitasi terhadap Rendemen
dan Sifat Karagenan dari Rumput laut Eucheuma cottoni.
Ekuilibrium. Vol. 8, No. 2:47-53.
[6] Mtolera, M.S., and Buriyo, A.S., 2004, Studies on Tanzanian
Hypneaceae: Seasonal Variation in Content and Quality of Kappa-
Carrageenan from Hynea musciformis, Western Indian Ocean J.
Mar. Sci., 3, pp. 43-49
[7] Pelegrin, Y.F., Robledo, D., and Azamar, J.A.,2006, Carrageenan of
Eucheuma isiforme (Solieriaceae, Rhodophyta) from Yucatanm
Mexico. I. Effect of Extraction Conditions, Botanica Marina, 49, pp.
65-71.
[8] AOAC. 1995. Official Methods of Analysis of the Association of
Official Analytical Chemist. Virginia: AOAC Inc. Arlington.
[9] Andriani D. 2006. Pengolahan Rumput Laut (Eucheuma cottonii)
Menjadi Tepung ATC (Alkali Treated Cottonii) dengan Jenis dan
Konsentrasi Larutan Alkali yang Berbeda. (undergraduate thesis).
Makassar: Faculty of Agriculture and Forestry, Universitas
Hasanudin.
[10] Distantina, S., Fadhilah, Rochmadi, Wiratni, M. Fahrurozi. 2010.
Proses Ekstraksi Karagenan dari Eucheuma cottonii. Seminar
Rekayasa Kimia dan Proses. Department E. H. Miller, “A note on
reflector arrays (Periodical style—Accepted for publication),” IEEE
Trans. Antennas Propagat., to be published.
[11] Syamsuar. 2006. Karakteristik Karaginan Rumput Laut Eucheuma
Cottonii pada Berbagai Umur Panen, Konsentrasi KOH dan Lama
Ekstraksi. (Undergraduate thesis). Bogor: Graduate School of IPB.
[12] Wang, W. C. 1995. Ohmic Heating of Foods: Physical Properties
and Applications. (Dissertation). Colombus: The Ohio State
University.
[13] Ary, A. P. 2015. Studi Pengaruh Metode Presipitasi terhadap
Rendemen yang dihasilkan dari Proses Ekstraksi Rumput Laut
Eucheuma cottonii Melalui Pemanasan OHMIC. (Skripsi).
Makassar: Universitas Hassanudin.
[14] Moirano, A. L. 1977. Sulfated Seaweed Polysacharides in Food
Colloids. Westport, Connecticut: The AVI Publishing Company.
[15] Ningsih, F. L. 2014. Jenis dan Konsentrasi Alkali dengan Presipitasi
KCl yang Berbeda terhadap Mutu Karaginan dari Rumput Laut
Kappaphycus alvarezii Asal Pulo Panjang Serang Banten.
(Undergraduate thesis). Banten: Universitas Sultan Ageng Tirtayasa.
[16] Romenda, A. P., R. Pramesti, A. B. Susanto. 2013. Pengaruh
Perbedaan Jenis dan Konsentrasi Larutan Alkali terhadap Kekuatan
Gel dan Viskositas Karaginan Kappaphycus alvarezii Doty. Journal
of Marine Research. Vol. 2, No. 1:127-133
[17] Food and Agriculture Organization (FAO). 2007. Carrageenan. FAO
JECFA Monogaphs 4
[18] Winarno. F. G. 1990. Kimia Pangan dan Gizi. Jakarta: Gramedia
Pustaka Utama.
[19] Winarno, F. G., S. Fardiaz, and D. Fardiaz. 1996. Teknologi
Pengolahan Rumput Laut. Jakarta: Putaka Sinar Harapan.
